Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography
Complexation by dissolved organic ligands affects the bioavailability and distribution of copper and other bioactive trace metals in seawater. However, relatively little is known about the origin and identity of marine copper-complexing ligands, particularly in Arctic waters. We used immobilized cop...
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Marine Chemistry
2019
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Online Access: | http://hdl.handle.net/1828/11469 https://doi.org/10.1016/j.marchem.2019.103673 |
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ftuvicpubl:oai:dspace.library.uvic.ca:1828/11469 2023-05-15T14:25:04+02:00 Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography Nixon, Richard L. Sarah, Jackson Cullen, Jay T. Ross, Andrew 2019 application/pdf http://hdl.handle.net/1828/11469 https://doi.org/10.1016/j.marchem.2019.103673 en eng Marine Chemistry Nixon, R.L., Jackson, S.L., Cullen, J.T. & Ross, A.R.S. (2019). Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography. Marine Chemistry, 215, 103673. https://doi.org/10.1016/j.marchem.2019.103673 https://doi.org/10.1016/j.marchem.2019.103673 http://hdl.handle.net/1828/11469 Marine organic ligands Copper Immobilized metal-ion affinity chromatography Canadian Arctic GEOTRACES Article 2019 ftuvicpubl https://doi.org/10.1016/j.marchem.2019.103673 2022-05-19T06:14:18Z Complexation by dissolved organic ligands affects the bioavailability and distribution of copper and other bioactive trace metals in seawater. However, relatively little is known about the origin and identity of marine copper-complexing ligands, particularly in Arctic waters. We used immobilized copper(II)-ion affinity chromatography (IMAC) to isolate dissolved (< 0.2 μm) copper ligands from seawater samples collected during the 2015 Canadian Arctic GEOTRACES expedition. UV detection at 254 nm was employed to monitor elution of the compounds retained by IMAC. The areas of the resulting peaks were used to generate depth profiles that show, for the first time, how copper ligands are distributed across the Canadian Arctic. Copper ligand concentrations ranged from 0.9 to 4.8 nM, the depth of highest ligand concentration often coinciding with the chlorophyll maximum depth. Correlations between ligand concentration and in situ chlorophyll-a fluorescence suggest that marine phytoplankton or cyanobacteria could be an important source of copper ligands. A correlation was also observed between copper ligand and dissolved copper concentrations in Baffin Bay. Comparison of these results with published data for humic substances and other dissolved organic matter suggests that terrestrial input contributes to the pool of ligands captured by IMAC, particularly in the Canada Basin and Canadian Arctic Archipelago. This work was supported by the NSERC Climate Change and Atmospheric Research (CCAR) program (Grant Number RGPCC 433848-2012) and Fisheries and Oceans Canada (AR) and by university and departmental graduate scholarships from the University of Victoria (RN). Faculty Reviewed Article in Journal/Newspaper Arctic Arctic Archipelago Arctic Baffin Bay Baffin Bay Baffin canada basin Canadian Arctic Archipelago Climate change Phytoplankton University of Victoria (Canada): UVicDSpace Arctic Baffin Bay Canada Canadian Arctic Archipelago Marine Chemistry 215 103673 |
institution |
Open Polar |
collection |
University of Victoria (Canada): UVicDSpace |
op_collection_id |
ftuvicpubl |
language |
English |
topic |
Marine organic ligands Copper Immobilized metal-ion affinity chromatography Canadian Arctic GEOTRACES |
spellingShingle |
Marine organic ligands Copper Immobilized metal-ion affinity chromatography Canadian Arctic GEOTRACES Nixon, Richard L. Sarah, Jackson Cullen, Jay T. Ross, Andrew Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography |
topic_facet |
Marine organic ligands Copper Immobilized metal-ion affinity chromatography Canadian Arctic GEOTRACES |
description |
Complexation by dissolved organic ligands affects the bioavailability and distribution of copper and other bioactive trace metals in seawater. However, relatively little is known about the origin and identity of marine copper-complexing ligands, particularly in Arctic waters. We used immobilized copper(II)-ion affinity chromatography (IMAC) to isolate dissolved (< 0.2 μm) copper ligands from seawater samples collected during the 2015 Canadian Arctic GEOTRACES expedition. UV detection at 254 nm was employed to monitor elution of the compounds retained by IMAC. The areas of the resulting peaks were used to generate depth profiles that show, for the first time, how copper ligands are distributed across the Canadian Arctic. Copper ligand concentrations ranged from 0.9 to 4.8 nM, the depth of highest ligand concentration often coinciding with the chlorophyll maximum depth. Correlations between ligand concentration and in situ chlorophyll-a fluorescence suggest that marine phytoplankton or cyanobacteria could be an important source of copper ligands. A correlation was also observed between copper ligand and dissolved copper concentrations in Baffin Bay. Comparison of these results with published data for humic substances and other dissolved organic matter suggests that terrestrial input contributes to the pool of ligands captured by IMAC, particularly in the Canada Basin and Canadian Arctic Archipelago. This work was supported by the NSERC Climate Change and Atmospheric Research (CCAR) program (Grant Number RGPCC 433848-2012) and Fisheries and Oceans Canada (AR) and by university and departmental graduate scholarships from the University of Victoria (RN). Faculty Reviewed |
format |
Article in Journal/Newspaper |
author |
Nixon, Richard L. Sarah, Jackson Cullen, Jay T. Ross, Andrew |
author_facet |
Nixon, Richard L. Sarah, Jackson Cullen, Jay T. Ross, Andrew |
author_sort |
Nixon, Richard L. |
title |
Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography |
title_short |
Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography |
title_full |
Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography |
title_fullStr |
Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography |
title_full_unstemmed |
Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography |
title_sort |
distribution of copper-complexing ligands in canadian arctic waters as determined by immobilized copper(ii)-ion affinity chromatography |
publisher |
Marine Chemistry |
publishDate |
2019 |
url |
http://hdl.handle.net/1828/11469 https://doi.org/10.1016/j.marchem.2019.103673 |
geographic |
Arctic Baffin Bay Canada Canadian Arctic Archipelago |
geographic_facet |
Arctic Baffin Bay Canada Canadian Arctic Archipelago |
genre |
Arctic Arctic Archipelago Arctic Baffin Bay Baffin Bay Baffin canada basin Canadian Arctic Archipelago Climate change Phytoplankton |
genre_facet |
Arctic Arctic Archipelago Arctic Baffin Bay Baffin Bay Baffin canada basin Canadian Arctic Archipelago Climate change Phytoplankton |
op_relation |
Nixon, R.L., Jackson, S.L., Cullen, J.T. & Ross, A.R.S. (2019). Distribution of copper-complexing ligands in Canadian Arctic waters as determined by immobilized copper(II)-ion affinity chromatography. Marine Chemistry, 215, 103673. https://doi.org/10.1016/j.marchem.2019.103673 https://doi.org/10.1016/j.marchem.2019.103673 http://hdl.handle.net/1828/11469 |
op_doi |
https://doi.org/10.1016/j.marchem.2019.103673 |
container_title |
Marine Chemistry |
container_volume |
215 |
container_start_page |
103673 |
_version_ |
1766297505769717760 |