Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus
The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that ∼26% of the exci...
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Neuropharmacology
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Online Access: | http://hdl.handle.net/1828/10924 https://doi.org/10.1016/j.neuropharm.2019.04.020 |
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ftuvicpubl:oai:dspace.library.uvic.ca:1828/10924 2023-05-15T16:01:46+02:00 Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus Peñasco, Sara Rico-Barrio, Irantzu Puente, Nagore Gómez-Urquijo, Sonia María Fontaine, Christine J. Egaña-Huguet, Jon Achicallende, Svein Ramos, Almudena Reguero, Leire Elezgarai, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes, Pedro 2019 application/pdf http://hdl.handle.net/1828/10924 https://doi.org/10.1016/j.neuropharm.2019.04.020 en eng Neuropharmacology Peñasco , S., Rico-Barrio, I., Puente, N., Gómez-Urquijo, S.M., Fontaine, C.J., Egaña-Huguet, J., … Grandes, P. (2019). Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus. Neuropharmacology, 153, 32-40. https://doi.org/10.1016/j.neuropharm.2019.04.020 https://doi.org/10.1016/j.neuropharm.2019.04.020 http://hdl.handle.net/1828/10924 CB1 receptor 2-AG Electrophysiology Excitatory synapses Long-term depression Hippocampus Article 2019 ftuvicpubl https://doi.org/10.1016/j.neuropharm.2019.04.020 2022-05-19T06:10:56Z The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that ∼26% of the excitatory synaptic terminals in the middle 1/3 of the dentate molecular layer (DML) contained CB1 receptors, and field excitatory postsynaptic potentials evoked by MPP stimulation were inhibited by CB1 receptor activation. In addition, MPP stimulation at 10 Hz for 10 min triggered CB1 receptor-dependent excitatory long-term depression (eCB-eLTD) at MPP synapses of wild-type mice but not on CB1-knockout mice. This eCB-eLTD was group I mGluR-dependent, required intracellular calcium influx and 2-arachydonoyl-glycerol (2-AG) synthesis but did not depend on N-methyl-d-aspartate (NMDA) receptors. Overall, these results point to a functional role for CB1 receptors with eCB-eLTD at DML MPP synapses and further involve these receptors in memory processing within the adult brain. This work was supported by MINECO/FEDER, UE (grant number SAF2015-65034-R to PG); The Basque Government (grant number BCG IT764-13 to PG); Red de Trastornos Adictivos, Instituto de Salud Carlos III (ISC-III) and European Regional Development Funds-European Union (ERDF-EU; grant RD16/0017/0012 to PG); PhD contract from MINECO (BES-2013-065057 to SP); Vanier Canada Graduate Scholarship (NSERC to CJF). Faculty Reviewed Article in Journal/Newspaper DML University of Victoria (Canada): UVicDSpace Canada Neuropharmacology 153 32 40 |
institution |
Open Polar |
collection |
University of Victoria (Canada): UVicDSpace |
op_collection_id |
ftuvicpubl |
language |
English |
topic |
CB1 receptor 2-AG Electrophysiology Excitatory synapses Long-term depression Hippocampus |
spellingShingle |
CB1 receptor 2-AG Electrophysiology Excitatory synapses Long-term depression Hippocampus Peñasco, Sara Rico-Barrio, Irantzu Puente, Nagore Gómez-Urquijo, Sonia María Fontaine, Christine J. Egaña-Huguet, Jon Achicallende, Svein Ramos, Almudena Reguero, Leire Elezgarai, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes, Pedro Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
topic_facet |
CB1 receptor 2-AG Electrophysiology Excitatory synapses Long-term depression Hippocampus |
description |
The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that ∼26% of the excitatory synaptic terminals in the middle 1/3 of the dentate molecular layer (DML) contained CB1 receptors, and field excitatory postsynaptic potentials evoked by MPP stimulation were inhibited by CB1 receptor activation. In addition, MPP stimulation at 10 Hz for 10 min triggered CB1 receptor-dependent excitatory long-term depression (eCB-eLTD) at MPP synapses of wild-type mice but not on CB1-knockout mice. This eCB-eLTD was group I mGluR-dependent, required intracellular calcium influx and 2-arachydonoyl-glycerol (2-AG) synthesis but did not depend on N-methyl-d-aspartate (NMDA) receptors. Overall, these results point to a functional role for CB1 receptors with eCB-eLTD at DML MPP synapses and further involve these receptors in memory processing within the adult brain. This work was supported by MINECO/FEDER, UE (grant number SAF2015-65034-R to PG); The Basque Government (grant number BCG IT764-13 to PG); Red de Trastornos Adictivos, Instituto de Salud Carlos III (ISC-III) and European Regional Development Funds-European Union (ERDF-EU; grant RD16/0017/0012 to PG); PhD contract from MINECO (BES-2013-065057 to SP); Vanier Canada Graduate Scholarship (NSERC to CJF). Faculty Reviewed |
format |
Article in Journal/Newspaper |
author |
Peñasco, Sara Rico-Barrio, Irantzu Puente, Nagore Gómez-Urquijo, Sonia María Fontaine, Christine J. Egaña-Huguet, Jon Achicallende, Svein Ramos, Almudena Reguero, Leire Elezgarai, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes, Pedro |
author_facet |
Peñasco, Sara Rico-Barrio, Irantzu Puente, Nagore Gómez-Urquijo, Sonia María Fontaine, Christine J. Egaña-Huguet, Jon Achicallende, Svein Ramos, Almudena Reguero, Leire Elezgarai, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes, Pedro |
author_sort |
Peñasco, Sara |
title |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_short |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_full |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_fullStr |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_full_unstemmed |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_sort |
endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
publisher |
Neuropharmacology |
publishDate |
2019 |
url |
http://hdl.handle.net/1828/10924 https://doi.org/10.1016/j.neuropharm.2019.04.020 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
DML |
genre_facet |
DML |
op_relation |
Peñasco , S., Rico-Barrio, I., Puente, N., Gómez-Urquijo, S.M., Fontaine, C.J., Egaña-Huguet, J., … Grandes, P. (2019). Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus. Neuropharmacology, 153, 32-40. https://doi.org/10.1016/j.neuropharm.2019.04.020 https://doi.org/10.1016/j.neuropharm.2019.04.020 http://hdl.handle.net/1828/10924 |
op_doi |
https://doi.org/10.1016/j.neuropharm.2019.04.020 |
container_title |
Neuropharmacology |
container_volume |
153 |
container_start_page |
32 |
op_container_end_page |
40 |
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1766397497036505088 |