Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile
International audience Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw a...
Published in: | Diversity |
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Main Authors: | , , , , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2021
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Online Access: | https://cnrs.hal.science/hal-03974645 https://cnrs.hal.science/hal-03974645/document https://cnrs.hal.science/hal-03974645/file/diversity-13-00328-v3.pdf https://doi.org/10.3390/d13070328 |
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ftutoulouse3hal:oai:HAL:hal-03974645v1 |
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openpolar |
institution |
Open Polar |
collection |
Université Toulouse III - Paul Sabatier: HAL-UPS |
op_collection_id |
ftutoulouse3hal |
language |
English |
topic |
permafrost peat pore water bacteria number metabolism active layer [SDE]Environmental Sciences |
spellingShingle |
permafrost peat pore water bacteria number metabolism active layer [SDE]Environmental Sciences Aksenov, Andrey Shirokova, Liudmila Kisil, Oksana Ya. Kolesova, Sofiia Lim, Artem Kuzmina, Darya Pouillé, Sophie Alexis, Marie Castrec-Rouelle, Maryse Loiko, Sergey Pokrovsky, Oleg Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
topic_facet |
permafrost peat pore water bacteria number metabolism active layer [SDE]Environmental Sciences |
description |
International audience Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation. Peat microorganisms are largely responsible for the processing of this OC, yet coupled studies of chemical and bacterial parameters in permafrost peatlands are rather limited and geographically biased. Towards testing the possible impact of peat and peat pore water chemical composition on microbial population and diversity, here we present results of a preliminary study of the western Siberia permafrost peatland discontinuous permafrost zone. The quantitative evaluation of microorganisms and determination of microbial diversity along a 100 cm thick peat soil column, which included thawed and frozen peat and bottom mineral horizon, was performed by RT-PCR and 16S rRNA gene-based metagenomic analysis, respectively. Bacteria (mainly Proteobacteria, Acidobacteria, Actinobacteria) strongly dominated the microbial diversity (99% sequences), with a negligible proportion of archaea (0.3–0.5%). There was a systematic evolution of main taxa according to depth, with a maximum of 65% (Acidobacteria) encountered in the active layer, or permafrost boundary (50–60 cm). We also measured C, N, nutrients and ~50 major and trace elements in peat (19 samples) as well as its pore water and dispersed ice (10 samples), sampled over the same core, and we analyzed organic matter quality in six organic and one mineral horizon of this core. Using multiparametric statistics (PCA), we tested the links between the total microbial number and 16S rRNA diversity and chemical composition of both the solid and fluid phase harboring the microorganisms. Under climate warming and permafrost thaw, one can expect a downward movement of the layer of maximal genetic diversity following the active layer thickening. Given a one to two ... |
author2 |
Northern Arctic Federal University, Northern Dvina Emb 17, 163002, Arkhangelsk, Russia Géosciences Environnement Toulouse (GET) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Centre National de la Recherche Scientifique (CNRS) Tomsk State University Tomsk Sorbonne Université (SU) |
format |
Article in Journal/Newspaper |
author |
Aksenov, Andrey Shirokova, Liudmila Kisil, Oksana Ya. Kolesova, Sofiia Lim, Artem Kuzmina, Darya Pouillé, Sophie Alexis, Marie Castrec-Rouelle, Maryse Loiko, Sergey Pokrovsky, Oleg |
author_facet |
Aksenov, Andrey Shirokova, Liudmila Kisil, Oksana Ya. Kolesova, Sofiia Lim, Artem Kuzmina, Darya Pouillé, Sophie Alexis, Marie Castrec-Rouelle, Maryse Loiko, Sergey Pokrovsky, Oleg |
author_sort |
Aksenov, Andrey |
title |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_short |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_full |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_fullStr |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_full_unstemmed |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_sort |
bacterial number and genetic diversity in a permafrost peatland (western siberia): testing a link with organic matter quality and elementary composition of a peat soil profile |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://cnrs.hal.science/hal-03974645 https://cnrs.hal.science/hal-03974645/document https://cnrs.hal.science/hal-03974645/file/diversity-13-00328-v3.pdf https://doi.org/10.3390/d13070328 |
genre |
Ice permafrost Siberia |
genre_facet |
Ice permafrost Siberia |
op_source |
ISSN: 1424-2818 Diversity https://cnrs.hal.science/hal-03974645 Diversity, 2021, 13 (7), pp.328. ⟨10.3390/d13070328⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/d13070328 hal-03974645 https://cnrs.hal.science/hal-03974645 https://cnrs.hal.science/hal-03974645/document https://cnrs.hal.science/hal-03974645/file/diversity-13-00328-v3.pdf doi:10.3390/d13070328 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/d13070328 |
container_title |
Diversity |
container_volume |
13 |
container_issue |
7 |
container_start_page |
328 |
_version_ |
1810449115263270912 |
spelling |
ftutoulouse3hal:oai:HAL:hal-03974645v1 2024-09-15T18:11:31+00:00 Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile Aksenov, Andrey Shirokova, Liudmila Kisil, Oksana Ya. Kolesova, Sofiia Lim, Artem Kuzmina, Darya Pouillé, Sophie Alexis, Marie Castrec-Rouelle, Maryse Loiko, Sergey Pokrovsky, Oleg Northern Arctic Federal University, Northern Dvina Emb 17, 163002, Arkhangelsk, Russia Géosciences Environnement Toulouse (GET) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Centre National de la Recherche Scientifique (CNRS) Tomsk State University Tomsk Sorbonne Université (SU) 2021-07 https://cnrs.hal.science/hal-03974645 https://cnrs.hal.science/hal-03974645/document https://cnrs.hal.science/hal-03974645/file/diversity-13-00328-v3.pdf https://doi.org/10.3390/d13070328 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/d13070328 hal-03974645 https://cnrs.hal.science/hal-03974645 https://cnrs.hal.science/hal-03974645/document https://cnrs.hal.science/hal-03974645/file/diversity-13-00328-v3.pdf doi:10.3390/d13070328 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1424-2818 Diversity https://cnrs.hal.science/hal-03974645 Diversity, 2021, 13 (7), pp.328. ⟨10.3390/d13070328⟩ permafrost peat pore water bacteria number metabolism active layer [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2021 ftutoulouse3hal https://doi.org/10.3390/d13070328 2024-06-25T00:02:29Z International audience Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation. Peat microorganisms are largely responsible for the processing of this OC, yet coupled studies of chemical and bacterial parameters in permafrost peatlands are rather limited and geographically biased. Towards testing the possible impact of peat and peat pore water chemical composition on microbial population and diversity, here we present results of a preliminary study of the western Siberia permafrost peatland discontinuous permafrost zone. The quantitative evaluation of microorganisms and determination of microbial diversity along a 100 cm thick peat soil column, which included thawed and frozen peat and bottom mineral horizon, was performed by RT-PCR and 16S rRNA gene-based metagenomic analysis, respectively. Bacteria (mainly Proteobacteria, Acidobacteria, Actinobacteria) strongly dominated the microbial diversity (99% sequences), with a negligible proportion of archaea (0.3–0.5%). There was a systematic evolution of main taxa according to depth, with a maximum of 65% (Acidobacteria) encountered in the active layer, or permafrost boundary (50–60 cm). We also measured C, N, nutrients and ~50 major and trace elements in peat (19 samples) as well as its pore water and dispersed ice (10 samples), sampled over the same core, and we analyzed organic matter quality in six organic and one mineral horizon of this core. Using multiparametric statistics (PCA), we tested the links between the total microbial number and 16S rRNA diversity and chemical composition of both the solid and fluid phase harboring the microorganisms. Under climate warming and permafrost thaw, one can expect a downward movement of the layer of maximal genetic diversity following the active layer thickening. Given a one to two ... Article in Journal/Newspaper Ice permafrost Siberia Université Toulouse III - Paul Sabatier: HAL-UPS Diversity 13 7 328 |