DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses
Molecular forensics is an important component of wildlife research and management. Using DNA from noninvasive samples collected at predation sites, we can identify predator species and obtain individual genotypes, improving our understanding of predator–prey dynamics and impacts of predators on live...
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ftutahsudc:oai:digitalcommons.usu.edu:wild_facpub-4044 2023-05-15T15:50:38+02:00 DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses Piaggio, Antoinette J. Shriner, Susan A. Young, Julie K. Griffin, Doreen L. Callahan, Peggy Wostenberg, Darren J. Gese, Eric M. Hopken, Matthew W. Oxford University Press 2019-11-07T08:00:00Z application/pdf https://digitalcommons.usu.edu/wild_facpub/3044 https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=4044&context=wild_facpub unknown Hosted by Utah State University Libraries https://digitalcommons.usu.edu/wild_facpub/3044 https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=4044&context=wild_facpub Copyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact the Institutional Repository Librarian at digitalcommons@usu.edu. http://creativecommons.org/publicdomain/mark/1.0/ PDM Wildland Resources Faculty Publications depredation DNA persistence noninvasive DNA predators salivary DNA Ecology and Evolutionary Biology text 2019 ftutahsudc 2022-10-27T17:21:57Z Molecular forensics is an important component of wildlife research and management. Using DNA from noninvasive samples collected at predation sites, we can identify predator species and obtain individual genotypes, improving our understanding of predator–prey dynamics and impacts of predators on livestock and endangered species. To improve sample collection strategies, we tested two sample collection methods and estimated degradation rates of predator DNA on the carcasses of multiple prey species. We fed carcasses of calves (Bos taurus) and lambs (Ovis aires) to three captive predator species: wolves (Canis lupus), coyotes (C. latrans), and mountain lions (Puma concolor). We swabbed the carcass in the field, as well as removed a piece of hide from the carcasses and then swabbed it in the laboratory. We swabbed all tissue samples through time and attempted to identify the predator involved in the depredation using salivary DNA. We found the most successful approach for yielding viable salivary DNA was removing hide from the prey and swabbing it in the laboratory. As expected, genotyping error increased through time and our ability to obtain complete genotypes decreased over time, the latter falling below 50% after 24 h. We provide guidelines for sampling salivary DNA from tissues of depredated carcasses for maximum probability of detection. Text Canis lupus Utah State University: DigitalCommons@USU |
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Utah State University: DigitalCommons@USU |
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ftutahsudc |
language |
unknown |
topic |
depredation DNA persistence noninvasive DNA predators salivary DNA Ecology and Evolutionary Biology |
spellingShingle |
depredation DNA persistence noninvasive DNA predators salivary DNA Ecology and Evolutionary Biology Piaggio, Antoinette J. Shriner, Susan A. Young, Julie K. Griffin, Doreen L. Callahan, Peggy Wostenberg, Darren J. Gese, Eric M. Hopken, Matthew W. DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses |
topic_facet |
depredation DNA persistence noninvasive DNA predators salivary DNA Ecology and Evolutionary Biology |
description |
Molecular forensics is an important component of wildlife research and management. Using DNA from noninvasive samples collected at predation sites, we can identify predator species and obtain individual genotypes, improving our understanding of predator–prey dynamics and impacts of predators on livestock and endangered species. To improve sample collection strategies, we tested two sample collection methods and estimated degradation rates of predator DNA on the carcasses of multiple prey species. We fed carcasses of calves (Bos taurus) and lambs (Ovis aires) to three captive predator species: wolves (Canis lupus), coyotes (C. latrans), and mountain lions (Puma concolor). We swabbed the carcass in the field, as well as removed a piece of hide from the carcasses and then swabbed it in the laboratory. We swabbed all tissue samples through time and attempted to identify the predator involved in the depredation using salivary DNA. We found the most successful approach for yielding viable salivary DNA was removing hide from the prey and swabbing it in the laboratory. As expected, genotyping error increased through time and our ability to obtain complete genotypes decreased over time, the latter falling below 50% after 24 h. We provide guidelines for sampling salivary DNA from tissues of depredated carcasses for maximum probability of detection. |
author2 |
Oxford University Press |
format |
Text |
author |
Piaggio, Antoinette J. Shriner, Susan A. Young, Julie K. Griffin, Doreen L. Callahan, Peggy Wostenberg, Darren J. Gese, Eric M. Hopken, Matthew W. |
author_facet |
Piaggio, Antoinette J. Shriner, Susan A. Young, Julie K. Griffin, Doreen L. Callahan, Peggy Wostenberg, Darren J. Gese, Eric M. Hopken, Matthew W. |
author_sort |
Piaggio, Antoinette J. |
title |
DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses |
title_short |
DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses |
title_full |
DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses |
title_fullStr |
DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses |
title_full_unstemmed |
DNA Persistence in Predator Saliva from Multiple Species and Methods for Optimal Recovery from Depredated Carcasses |
title_sort |
dna persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
publisher |
Hosted by Utah State University Libraries |
publishDate |
2019 |
url |
https://digitalcommons.usu.edu/wild_facpub/3044 https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=4044&context=wild_facpub |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Wildland Resources Faculty Publications |
op_relation |
https://digitalcommons.usu.edu/wild_facpub/3044 https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=4044&context=wild_facpub |
op_rights |
Copyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact the Institutional Repository Librarian at digitalcommons@usu.edu. http://creativecommons.org/publicdomain/mark/1.0/ |
op_rightsnorm |
PDM |
_version_ |
1766385629600415744 |