Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis.
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to me...
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ftusurrey:oai:epubs.surrey.ac.uk:853394 2023-05-15T15:56:35+02:00 Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. Hoettges, Kai Henslee, Erin Torcal Serrano, Ruth M. Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Christopher Van Der Veen, Daan Labeed, Fatima Hughes, Michael 2019-12-16 text http://epubs.surrey.ac.uk/853394/1/2019%20Sci%20Rep.pdf https://doi.org/10.1038/s41598-019-55579-9 en eng Nature Research http://epubs.surrey.ac.uk/853394/ https://www.nature.com/articles/s41598-019-55579-9#Abs1 https://doi.org/10.1038/s41598-019-55579-9 http://epubs.surrey.ac.uk/853394/1/2019%20Sci%20Rep.pdf Hoettges, Kai, Henslee, Erin, Torcal Serrano, Ruth M., Jabr, Rita, Abdallat, Rula, Beale, Andrew, Waheed, Abdul, Camelliti, Patrizia, Fry, Christopher, Van Der Veen, Daan, Labeed, Fatima and Hughes, Michael (2019) Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. Scientific Reports, 9, 19153. Article PeerReviewed 2019 ftusurrey https://doi.org/10.1038/s41598-019-55579-9 2020-02-20T23:10:28Z Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2 > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. Article in Journal/Newspaper Common vole Microtus arvalis University of Surrey, Guildford: Surrey Scholarship Online. Scientific Reports 9 1 |
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Open Polar |
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University of Surrey, Guildford: Surrey Scholarship Online. |
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ftusurrey |
language |
English |
description |
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2 > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. |
format |
Article in Journal/Newspaper |
author |
Hoettges, Kai Henslee, Erin Torcal Serrano, Ruth M. Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Christopher Van Der Veen, Daan Labeed, Fatima Hughes, Michael |
spellingShingle |
Hoettges, Kai Henslee, Erin Torcal Serrano, Ruth M. Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Christopher Van Der Veen, Daan Labeed, Fatima Hughes, Michael Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. |
author_facet |
Hoettges, Kai Henslee, Erin Torcal Serrano, Ruth M. Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Christopher Van Der Veen, Daan Labeed, Fatima Hughes, Michael |
author_sort |
Hoettges, Kai |
title |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. |
title_short |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. |
title_full |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. |
title_fullStr |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. |
title_full_unstemmed |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. |
title_sort |
ten–second electrophysiology: evaluation of the 3dep platform for high-speed, high-accuracy cell analysis. |
publisher |
Nature Research |
publishDate |
2019 |
url |
http://epubs.surrey.ac.uk/853394/1/2019%20Sci%20Rep.pdf https://doi.org/10.1038/s41598-019-55579-9 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_relation |
http://epubs.surrey.ac.uk/853394/ https://www.nature.com/articles/s41598-019-55579-9#Abs1 https://doi.org/10.1038/s41598-019-55579-9 http://epubs.surrey.ac.uk/853394/1/2019%20Sci%20Rep.pdf Hoettges, Kai, Henslee, Erin, Torcal Serrano, Ruth M., Jabr, Rita, Abdallat, Rula, Beale, Andrew, Waheed, Abdul, Camelliti, Patrizia, Fry, Christopher, Van Der Veen, Daan, Labeed, Fatima and Hughes, Michael (2019) Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. Scientific Reports, 9, 19153. |
op_doi |
https://doi.org/10.1038/s41598-019-55579-9 |
container_title |
Scientific Reports |
container_volume |
9 |
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1 |
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1766391982206222336 |