Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems
Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions...
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ftustrathclyde:oai:strathprints.strath.ac.uk:89658 2024-09-09T20:10:05+00:00 Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems Parra, Jonathan Jarmusch, Scott A. Duncan, Katherine R. 2024-04-25 text https://strathprints.strath.ac.uk/89658/ https://strathprints.strath.ac.uk/89658/1/Parra-etal-EM-2024-Multi-omics-analysis-of-antagonistic-interactions-among-free-living-Pseudonocardia.docx https://doi.org/10.1111/1462-2920.16635 en eng https://strathprints.strath.ac.uk/89658/1/Parra-etal-EM-2024-Multi-omics-analysis-of-antagonistic-interactions-among-free-living-Pseudonocardia.docx Parra, Jonathan <https://strathprints.strath.ac.uk/view/author/1163757.html> and Jarmusch, Scott A. and Duncan, Katherine R. <https://strathprints.strath.ac.uk/view/author/1090208.html> (2024 <https://strathprints.strath.ac.uk/view/year/2024.html>) Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. Environmental Microbiology <https://strathprints.strath.ac.uk/view/publications/Environmental_Microbiology.html>. ISSN 1462-2920 (In Press) Article PeerReviewed 2024 ftustrathclyde https://doi.org/10.1111/1462-2920.16635 2024-06-19T00:10:57Z Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions with other microorganisms and their mutualistic interactions with insects. However, the chemical ecology of free-living Pseudonocardia remains understudied. This study applies a multi-omics approach to investigate the chemical ecology of free-living actinomycetes from the genus Pseudonocardia. In a comparative genomics analysis, it was observed that the biosynthetic gene cluster family (GCF) distribution was influenced mainly by phylogenetic distance rather than the geographic or ecological origin of strains. This finding was also observed in the mass spectrometry-based metabolomic profiles of nine Pseudonocardia species isolated from marine sediments and two terrestrial species. Antagonist interactions between these eleven species were examined, and matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) was used to examine in situ chemical interactions between the Southern Ocean strains and their phylogenetically close relatives. Overall, it was demonstrated that phylogeny was the main predictor of antagonistic interactions among free-living Pseudonocardia. Moreover, two features at m/z 441.15 and m/z 332.20 were identified as metabolites related to these inter-species interactions. Article in Journal/Newspaper Southern Ocean University of Strathclyde Glasgow: Strathprints Southern Ocean Environmental Microbiology 26 6 |
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University of Strathclyde Glasgow: Strathprints |
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ftustrathclyde |
language |
English |
description |
Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions with other microorganisms and their mutualistic interactions with insects. However, the chemical ecology of free-living Pseudonocardia remains understudied. This study applies a multi-omics approach to investigate the chemical ecology of free-living actinomycetes from the genus Pseudonocardia. In a comparative genomics analysis, it was observed that the biosynthetic gene cluster family (GCF) distribution was influenced mainly by phylogenetic distance rather than the geographic or ecological origin of strains. This finding was also observed in the mass spectrometry-based metabolomic profiles of nine Pseudonocardia species isolated from marine sediments and two terrestrial species. Antagonist interactions between these eleven species were examined, and matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) was used to examine in situ chemical interactions between the Southern Ocean strains and their phylogenetically close relatives. Overall, it was demonstrated that phylogeny was the main predictor of antagonistic interactions among free-living Pseudonocardia. Moreover, two features at m/z 441.15 and m/z 332.20 were identified as metabolites related to these inter-species interactions. |
format |
Article in Journal/Newspaper |
author |
Parra, Jonathan Jarmusch, Scott A. Duncan, Katherine R. |
spellingShingle |
Parra, Jonathan Jarmusch, Scott A. Duncan, Katherine R. Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems |
author_facet |
Parra, Jonathan Jarmusch, Scott A. Duncan, Katherine R. |
author_sort |
Parra, Jonathan |
title |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems |
title_short |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems |
title_full |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems |
title_fullStr |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems |
title_full_unstemmed |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems |
title_sort |
multi-omics analysis of antagonistic interactions among free-living pseudonocardia from diverse ecosystems |
publishDate |
2024 |
url |
https://strathprints.strath.ac.uk/89658/ https://strathprints.strath.ac.uk/89658/1/Parra-etal-EM-2024-Multi-omics-analysis-of-antagonistic-interactions-among-free-living-Pseudonocardia.docx https://doi.org/10.1111/1462-2920.16635 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_relation |
https://strathprints.strath.ac.uk/89658/1/Parra-etal-EM-2024-Multi-omics-analysis-of-antagonistic-interactions-among-free-living-Pseudonocardia.docx Parra, Jonathan <https://strathprints.strath.ac.uk/view/author/1163757.html> and Jarmusch, Scott A. and Duncan, Katherine R. <https://strathprints.strath.ac.uk/view/author/1090208.html> (2024 <https://strathprints.strath.ac.uk/view/year/2024.html>) Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. Environmental Microbiology <https://strathprints.strath.ac.uk/view/publications/Environmental_Microbiology.html>. ISSN 1462-2920 (In Press) |
op_doi |
https://doi.org/10.1111/1462-2920.16635 |
container_title |
Environmental Microbiology |
container_volume |
26 |
container_issue |
6 |
_version_ |
1809944437772517376 |