Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply
Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood–oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (Pcrit), the PO2 below which oxygen supply cannot match...
Published in: | Journal of Experimental Biology |
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Digital Commons @ University of South Florida
2018
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Online Access: | https://digitalcommons.usf.edu/msc_facpub/1270 https://doi.org/10.1242/jeb.187443 |
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ftusouthflorida:oai:digitalcommons.usf.edu:msc_facpub-2293 2023-07-30T04:06:02+02:00 Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply Birk, Matthew A. McLean, Erin L. Seibel, Brad A. 2018-10-01T07:00:00Z https://digitalcommons.usf.edu/msc_facpub/1270 https://doi.org/10.1242/jeb.187443 unknown Digital Commons @ University of South Florida https://digitalcommons.usf.edu/msc_facpub/1270 doi:10.1242/jeb.187443 https://doi.org/10.1242/jeb.187443 Marine Science Faculty Publications Acid–base balance Blood–O2 binding Hypercapnia Cephalopod Hypoxia tolerance Dosidicus Life Sciences article 2018 ftusouthflorida https://doi.org/10.1242/jeb.187443 2023-07-13T21:02:38Z Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood–oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (Pcrit), the PO2 below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO2-induced reduction in oxygen supply should be apparent as an increase in Pcrit. In this study, we assessed the effects of CO2 (46–143 Pa; 455–1410 μatm) on the metabolic rate and Pcrit of two squid species – Dosidicus gigas and Doryteuthis pealeii – through manipulative experiments. We also developed a model, with inputs for hemocyanin pH sensitivity, blood PCO2 and buffering capacity, that simulates blood oxygen supply under varying seawater CO2 partial pressures. We compare model outputs with measured Pcrit in squid. Using blood–O2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid–base regulation, an increase in seawater PCO2 to 100 Pa (≈1000 μatm) would result in a maximum drop in arterial hemocyanin–O2 saturation by 1.6% at normoxia and a Pcrit increase of ≈0.5 kPa. Our live-animal experiments support this supposition, as CO2 had no effect on measured metabolic rate or Pcrit in either squid species. Article in Journal/Newspaper Ocean acidification University of South Florida St. Petersburg: Digital USFSP Journal of Experimental Biology |
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Open Polar |
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University of South Florida St. Petersburg: Digital USFSP |
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ftusouthflorida |
language |
unknown |
topic |
Acid–base balance Blood–O2 binding Hypercapnia Cephalopod Hypoxia tolerance Dosidicus Life Sciences |
spellingShingle |
Acid–base balance Blood–O2 binding Hypercapnia Cephalopod Hypoxia tolerance Dosidicus Life Sciences Birk, Matthew A. McLean, Erin L. Seibel, Brad A. Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply |
topic_facet |
Acid–base balance Blood–O2 binding Hypercapnia Cephalopod Hypoxia tolerance Dosidicus Life Sciences |
description |
Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood–oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (Pcrit), the PO2 below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO2-induced reduction in oxygen supply should be apparent as an increase in Pcrit. In this study, we assessed the effects of CO2 (46–143 Pa; 455–1410 μatm) on the metabolic rate and Pcrit of two squid species – Dosidicus gigas and Doryteuthis pealeii – through manipulative experiments. We also developed a model, with inputs for hemocyanin pH sensitivity, blood PCO2 and buffering capacity, that simulates blood oxygen supply under varying seawater CO2 partial pressures. We compare model outputs with measured Pcrit in squid. Using blood–O2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid–base regulation, an increase in seawater PCO2 to 100 Pa (≈1000 μatm) would result in a maximum drop in arterial hemocyanin–O2 saturation by 1.6% at normoxia and a Pcrit increase of ≈0.5 kPa. Our live-animal experiments support this supposition, as CO2 had no effect on measured metabolic rate or Pcrit in either squid species. |
format |
Article in Journal/Newspaper |
author |
Birk, Matthew A. McLean, Erin L. Seibel, Brad A. |
author_facet |
Birk, Matthew A. McLean, Erin L. Seibel, Brad A. |
author_sort |
Birk, Matthew A. |
title |
Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply |
title_short |
Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply |
title_full |
Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply |
title_fullStr |
Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply |
title_full_unstemmed |
Ocean Acidification does Not Limit Squid Metabolism via Blood Oxygen Supply |
title_sort |
ocean acidification does not limit squid metabolism via blood oxygen supply |
publisher |
Digital Commons @ University of South Florida |
publishDate |
2018 |
url |
https://digitalcommons.usf.edu/msc_facpub/1270 https://doi.org/10.1242/jeb.187443 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Marine Science Faculty Publications |
op_relation |
https://digitalcommons.usf.edu/msc_facpub/1270 doi:10.1242/jeb.187443 https://doi.org/10.1242/jeb.187443 |
op_doi |
https://doi.org/10.1242/jeb.187443 |
container_title |
Journal of Experimental Biology |
_version_ |
1772818404576591872 |