Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule
Fast protein size-exclusion liquid chromatography (SEC-FPLC) was used to study solvent-induced unfolding of six proteins. Two of them, sperm whale myoglobin and hen white lysozyme, denature on the simple N (native) to U (completely unfolded) scheme. The other four proteins - bovine and human α-lacta...
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1993
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ftusouthflorida:oai:digitalcommons.usf.edu:mme_facpub-1779 2023-11-12T04:26:51+01:00 Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule Uversky, Vladimir N. 1993-01-01T08:00:00Z https://digitalcommons.usf.edu/mme_facpub/622 https://doi.org/10.1021/bi00211a042 unknown Digital Commons @ University of South Florida https://digitalcommons.usf.edu/mme_facpub/622 doi:10.1021/bi00211a042 https://doi.org/10.1021/bi00211a042 Molecular Medicine Faculty Publications Medicine and Health Sciences text 1993 ftusouthflorida https://doi.org/10.1021/bi00211a042 2023-10-15T16:27:27Z Fast protein size-exclusion liquid chromatography (SEC-FPLC) was used to study solvent-induced unfolding of six proteins. Two of them, sperm whale myoglobin and hen white lysozyme, denature on the simple N (native) to U (completely unfolded) scheme. The other four proteins - bovine and human α-lactalbumin, bovine carbonic anhydrase B (BCAB), and β-lactamase from Staphylococcus aureus - denature through the molten globule (MG) state, i.e., on the N to MG to U denaturation scheme. We have shown that the permeation properties of the Superóse 12 columns are practically independent of temperature, pH, and denaturants in wide concentration intervals. In the case of myoglobin and lysozyme denaturation at 4°C (when the exchange between the native and unfolded states is slower than the characteristic time of chromatography), a bimodal distribution on molecular dimensions in the transition region was observed. This indicates that, under denaturant action, protein molecules can only be in one of the two states with different compactness. In other words, this shows that FPLC is one of the most direct approaches to establish the “all-or-none” mechanism of the equilibrium solvent-induced denaturation of globular proteins. The curves of guanidinium hydrochloride (GdmHCl) or urea-induced unfolding (N to U or MG to U transitions) of a protein on a column (monitored either by the relative areas of two peaks or—for fast exchange—by the position of the average peak) coincide with those monitored by far-UV CD in solution. The Stokes radius values obtained with the use of FPLC for the molten globule states of BCAB (1.6 M GdmHCl in 0.1 M sodium phosphate, pH 6.8, and acid form at pH 3.6) and for the human α-lactalbumin molten globule (2.0 M GdmHCl in 0.1 M sodium phosphate, pH 6.8) coincide with those known from literature. Text Sperm whale University of South Florida St. Petersburg: Digital USFSP Biochemistry 32 48 13288 13298 |
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University of South Florida St. Petersburg: Digital USFSP |
op_collection_id |
ftusouthflorida |
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unknown |
topic |
Medicine and Health Sciences |
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Medicine and Health Sciences Uversky, Vladimir N. Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule |
topic_facet |
Medicine and Health Sciences |
description |
Fast protein size-exclusion liquid chromatography (SEC-FPLC) was used to study solvent-induced unfolding of six proteins. Two of them, sperm whale myoglobin and hen white lysozyme, denature on the simple N (native) to U (completely unfolded) scheme. The other four proteins - bovine and human α-lactalbumin, bovine carbonic anhydrase B (BCAB), and β-lactamase from Staphylococcus aureus - denature through the molten globule (MG) state, i.e., on the N to MG to U denaturation scheme. We have shown that the permeation properties of the Superóse 12 columns are practically independent of temperature, pH, and denaturants in wide concentration intervals. In the case of myoglobin and lysozyme denaturation at 4°C (when the exchange between the native and unfolded states is slower than the characteristic time of chromatography), a bimodal distribution on molecular dimensions in the transition region was observed. This indicates that, under denaturant action, protein molecules can only be in one of the two states with different compactness. In other words, this shows that FPLC is one of the most direct approaches to establish the “all-or-none” mechanism of the equilibrium solvent-induced denaturation of globular proteins. The curves of guanidinium hydrochloride (GdmHCl) or urea-induced unfolding (N to U or MG to U transitions) of a protein on a column (monitored either by the relative areas of two peaks or—for fast exchange—by the position of the average peak) coincide with those monitored by far-UV CD in solution. The Stokes radius values obtained with the use of FPLC for the molten globule states of BCAB (1.6 M GdmHCl in 0.1 M sodium phosphate, pH 6.8, and acid form at pH 3.6) and for the human α-lactalbumin molten globule (2.0 M GdmHCl in 0.1 M sodium phosphate, pH 6.8) coincide with those known from literature. |
format |
Text |
author |
Uversky, Vladimir N. |
author_facet |
Uversky, Vladimir N. |
author_sort |
Uversky, Vladimir N. |
title |
Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule |
title_short |
Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule |
title_full |
Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule |
title_fullStr |
Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule |
title_full_unstemmed |
Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which Denature Through the Molten Globule |
title_sort |
use of fast protein size-exclusion liquid chromatography to study the unfolding of proteins which denature through the molten globule |
publisher |
Digital Commons @ University of South Florida |
publishDate |
1993 |
url |
https://digitalcommons.usf.edu/mme_facpub/622 https://doi.org/10.1021/bi00211a042 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Molecular Medicine Faculty Publications |
op_relation |
https://digitalcommons.usf.edu/mme_facpub/622 doi:10.1021/bi00211a042 https://doi.org/10.1021/bi00211a042 |
op_doi |
https://doi.org/10.1021/bi00211a042 |
container_title |
Biochemistry |
container_volume |
32 |
container_issue |
48 |
container_start_page |
13288 |
op_container_end_page |
13298 |
_version_ |
1782340676997873664 |