Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae)
For this thesis our objectives were to establish an efficient method of ovarian synchronization and superstimulation in bison, and determine the effects of gonadotropin treatments on oocyte collection efficiency and quality in bison. In the first study we conducted two experiments to develop an effi...
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University of Saskatchewan
2011
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ftusaskatchewan:oai:harvest.usask.ca:10388/etd-08022011-164451 2023-05-15T15:44:48+02:00 Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) Palomino, Jesus Manuel Adams, Gregg Barth, Albert Muir, Gillian McCorkell, Robert Mapletoft, Reuben Woodbury, Murray May 2011 http://hdl.handle.net/10388/etd-08022011-164451 en_US eng University of Saskatchewan http://hdl.handle.net/10388/etd-08022011-164451 TC-SSU-08022011164451 wood bison oocyte collection superstimulation follicular wave emergence text Thesis 2011 ftusaskatchewan 2022-07-30T22:10:22Z For this thesis our objectives were to establish an efficient method of ovarian synchronization and superstimulation in bison, and determine the effects of gonadotropin treatments on oocyte collection efficiency and quality in bison. In the first study we conducted two experiments to develop an efficient protocol for synchronization of follicular wave emergence during the anovulatory season. In Experiment 1, we compared the synchronizing effect of follicular ablation (n = 9) and treatment with 2 mg estradiol (E-) 17β in oil (n = 10), while in Experiment 2, we compared follicular ablation (n = 9) and treatment with 2 mg E-17β + 100 mg progesterone (P4; n = 10). Results showed that the degree of synchrony did not differ between ablation and hormone treatment groups in either Experiment, but follicular wave emergence was more synchronous in both treatment groups compared to the untreated control phase. The second study was conducted to develop an efficient method for ovarian superstimulation and oocyte collection during the anovulatory and ovulatory seasons. During the anovulatory season, one experiment was conducted in two replicates to compare the superstimulatory effect of 2500 IU of eCG (n = 10) given intramuscularly vs two doses of 200 mg of pFSH each (n = 10) given subcutaneously. Additionally, the effect of 25 mg of pLH given 24 hours prior oocyte collection on oocyte quality and collection rate was evaluated for each superstimulatory treatment. Results showed that treatment with pFSH induced a higher superstimulatory response and more cumulus oocyte complexes (COC) collected than did eCG during the anovulatory season. Furthermore, treatment with pLH increased the proportion of expanded COC that were collected with ultrasound-guided follicular aspiration. Two experiments were conducted during the ovulatory season, to develop an efficient protocol for superstimulation and oocyte collection. In Experiment 1, we compared the effect of two intramuscular doses of 200 mg of pFSH in saline (n = 11) vs two ... Thesis Bison bison athabascae Wood Bison Bison bison bison University of Saskatchewan: eCommons@USASK |
institution |
Open Polar |
collection |
University of Saskatchewan: eCommons@USASK |
op_collection_id |
ftusaskatchewan |
language |
English |
topic |
wood bison oocyte collection superstimulation follicular wave emergence |
spellingShingle |
wood bison oocyte collection superstimulation follicular wave emergence Palomino, Jesus Manuel Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) |
topic_facet |
wood bison oocyte collection superstimulation follicular wave emergence |
description |
For this thesis our objectives were to establish an efficient method of ovarian synchronization and superstimulation in bison, and determine the effects of gonadotropin treatments on oocyte collection efficiency and quality in bison. In the first study we conducted two experiments to develop an efficient protocol for synchronization of follicular wave emergence during the anovulatory season. In Experiment 1, we compared the synchronizing effect of follicular ablation (n = 9) and treatment with 2 mg estradiol (E-) 17β in oil (n = 10), while in Experiment 2, we compared follicular ablation (n = 9) and treatment with 2 mg E-17β + 100 mg progesterone (P4; n = 10). Results showed that the degree of synchrony did not differ between ablation and hormone treatment groups in either Experiment, but follicular wave emergence was more synchronous in both treatment groups compared to the untreated control phase. The second study was conducted to develop an efficient method for ovarian superstimulation and oocyte collection during the anovulatory and ovulatory seasons. During the anovulatory season, one experiment was conducted in two replicates to compare the superstimulatory effect of 2500 IU of eCG (n = 10) given intramuscularly vs two doses of 200 mg of pFSH each (n = 10) given subcutaneously. Additionally, the effect of 25 mg of pLH given 24 hours prior oocyte collection on oocyte quality and collection rate was evaluated for each superstimulatory treatment. Results showed that treatment with pFSH induced a higher superstimulatory response and more cumulus oocyte complexes (COC) collected than did eCG during the anovulatory season. Furthermore, treatment with pLH increased the proportion of expanded COC that were collected with ultrasound-guided follicular aspiration. Two experiments were conducted during the ovulatory season, to develop an efficient protocol for superstimulation and oocyte collection. In Experiment 1, we compared the effect of two intramuscular doses of 200 mg of pFSH in saline (n = 11) vs two ... |
author2 |
Adams, Gregg Barth, Albert Muir, Gillian McCorkell, Robert Mapletoft, Reuben Woodbury, Murray |
format |
Thesis |
author |
Palomino, Jesus Manuel |
author_facet |
Palomino, Jesus Manuel |
author_sort |
Palomino, Jesus Manuel |
title |
Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) |
title_short |
Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) |
title_full |
Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) |
title_fullStr |
Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) |
title_full_unstemmed |
Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae) |
title_sort |
ovarian synchronization and superstimulation in wood bison (bison bison athabascae) |
publisher |
University of Saskatchewan |
publishDate |
2011 |
url |
http://hdl.handle.net/10388/etd-08022011-164451 |
genre |
Bison bison athabascae Wood Bison Bison bison bison |
genre_facet |
Bison bison athabascae Wood Bison Bison bison bison |
op_relation |
http://hdl.handle.net/10388/etd-08022011-164451 TC-SSU-08022011164451 |
_version_ |
1766379163458994176 |