Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans
A novel human coronavirus (HCoV-EMC/2012) was isolated from a man with acute pneumonia and renal failure in June 2012. This report describes the complete genome sequence, genome organization, and expression strategy of HCoV-EMC/2012 and its relation with known coronaviruses. The genome contains 30,1...
Published in: | mBio |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2012
|
Subjects: | |
Online Access: | https://pure.eur.nl/en/publications/79b27f15-cd4e-4e1e-aa13-c1de58095c87 https://doi.org/10.1128/mBio.00473-12 https://pure.eur.nl/ws/files/47480365/Repub_68528_O-A.pdf http://hdl.handle.net/1765/68528 |
id |
fturottercrispub:oai:pure.eur.nl:publications/79b27f15-cd4e-4e1e-aa13-c1de58095c87 |
---|---|
record_format |
openpolar |
spelling |
fturottercrispub:oai:pure.eur.nl:publications/79b27f15-cd4e-4e1e-aa13-c1de58095c87 2023-05-15T17:59:57+02:00 Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans van Boheemen, Sander de Graaf, Miranda Lauber, C Bestebroer, Theo Victor, Stalinraj Zaki, AM Osterhaus, Ab Haagmans, Bart Gorbalenya, AE Snijder, EJ Fouchier, Ron 2012 application/pdf https://pure.eur.nl/en/publications/79b27f15-cd4e-4e1e-aa13-c1de58095c87 https://doi.org/10.1128/mBio.00473-12 https://pure.eur.nl/ws/files/47480365/Repub_68528_O-A.pdf http://hdl.handle.net/1765/68528 und unknown info:eu-repo/semantics/openAccess van Boheemen , S , de Graaf , M , Lauber , C , Bestebroer , T , Victor , S , Zaki , AM , Osterhaus , A , Haagmans , B , Gorbalenya , AE , Snijder , EJ & Fouchier , R 2012 , ' Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans ' , mBio , vol. 3 , no. 6 . https://doi.org/10.1128/mBio.00473-12 /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being SDG 3 - Good Health and Well-being article 2012 fturottercrispub https://doi.org/10.1128/mBio.00473-12 2022-07-21T08:43:33Z A novel human coronavirus (HCoV-EMC/2012) was isolated from a man with acute pneumonia and renal failure in June 2012. This report describes the complete genome sequence, genome organization, and expression strategy of HCoV-EMC/2012 and its relation with known coronaviruses. The genome contains 30,119 nucleotides and contains at least 10 predicted open reading frames, 9 of which are predicted to be expressed from a nested set of seven subgenomic mRNAs. Phylogenetic analysis of the replicase gene of coronaviruses with completely sequenced genomes showed that HCoV-EMC/2012 is most closely related to Tylonycteris bat coronavirus HKU4 (BtCoV-HKU4) and Pipistrellus bat coronavirus HKU5 (BtCoV-HKU5), which prototype two species in lineage C of the genus Betacoronavirus. In accordance with the guidelines of the International Committee on Taxonomy of Viruses, and in view of the 75% and 77% amino acid sequence identity in 7 conserved replicase domains with BtCoV-HKU4 and BtCoV-HKU5, respectively, we propose that HCoV-EMC/2012 prototypes a novel species in the genus Betacoronavirus. HCoV-EMC/2012 may be most closely related to a coronavirus detected in Pipistrellus pipistrellus in The Netherlands, but because only a short sequence from the most conserved part of the RNA-dependent RNA polymerase-encoding region of the genome was reported for this bat virus, its genetic distance from HCoV-EMC remains uncertain. HCoV-EMC/2012 is the sixth coronavirus known to infect humans and the first human virus within betacoronavirus lineage C. IMPORTANCE Coronaviruses are capable of infecting humans and many animal species. Most infections caused by human coronaviruses are relatively mild. However, the outbreak of severe acute respiratory syndrome (SARS) caused by SARS-CoV in 2002 to 2003 and the fatal infection of a human by HCoV-EMC/2012 in 2012 show that coronaviruses are able to cause severe, sometimes fatal disease in humans. We have determined the complete genome of HCoV-EMC/2012 using an unbiased virus discovery approach involv Article in Journal/Newspaper Pipistrellus pipistrellus Erasmus University Rotterdam & Erasmus MC Research Portal mBio 3 6 |
institution |
Open Polar |
collection |
Erasmus University Rotterdam & Erasmus MC Research Portal |
op_collection_id |
fturottercrispub |
language |
unknown |
topic |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being SDG 3 - Good Health and Well-being |
spellingShingle |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being SDG 3 - Good Health and Well-being van Boheemen, Sander de Graaf, Miranda Lauber, C Bestebroer, Theo Victor, Stalinraj Zaki, AM Osterhaus, Ab Haagmans, Bart Gorbalenya, AE Snijder, EJ Fouchier, Ron Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans |
topic_facet |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being SDG 3 - Good Health and Well-being |
description |
A novel human coronavirus (HCoV-EMC/2012) was isolated from a man with acute pneumonia and renal failure in June 2012. This report describes the complete genome sequence, genome organization, and expression strategy of HCoV-EMC/2012 and its relation with known coronaviruses. The genome contains 30,119 nucleotides and contains at least 10 predicted open reading frames, 9 of which are predicted to be expressed from a nested set of seven subgenomic mRNAs. Phylogenetic analysis of the replicase gene of coronaviruses with completely sequenced genomes showed that HCoV-EMC/2012 is most closely related to Tylonycteris bat coronavirus HKU4 (BtCoV-HKU4) and Pipistrellus bat coronavirus HKU5 (BtCoV-HKU5), which prototype two species in lineage C of the genus Betacoronavirus. In accordance with the guidelines of the International Committee on Taxonomy of Viruses, and in view of the 75% and 77% amino acid sequence identity in 7 conserved replicase domains with BtCoV-HKU4 and BtCoV-HKU5, respectively, we propose that HCoV-EMC/2012 prototypes a novel species in the genus Betacoronavirus. HCoV-EMC/2012 may be most closely related to a coronavirus detected in Pipistrellus pipistrellus in The Netherlands, but because only a short sequence from the most conserved part of the RNA-dependent RNA polymerase-encoding region of the genome was reported for this bat virus, its genetic distance from HCoV-EMC remains uncertain. HCoV-EMC/2012 is the sixth coronavirus known to infect humans and the first human virus within betacoronavirus lineage C. IMPORTANCE Coronaviruses are capable of infecting humans and many animal species. Most infections caused by human coronaviruses are relatively mild. However, the outbreak of severe acute respiratory syndrome (SARS) caused by SARS-CoV in 2002 to 2003 and the fatal infection of a human by HCoV-EMC/2012 in 2012 show that coronaviruses are able to cause severe, sometimes fatal disease in humans. We have determined the complete genome of HCoV-EMC/2012 using an unbiased virus discovery approach involv |
format |
Article in Journal/Newspaper |
author |
van Boheemen, Sander de Graaf, Miranda Lauber, C Bestebroer, Theo Victor, Stalinraj Zaki, AM Osterhaus, Ab Haagmans, Bart Gorbalenya, AE Snijder, EJ Fouchier, Ron |
author_facet |
van Boheemen, Sander de Graaf, Miranda Lauber, C Bestebroer, Theo Victor, Stalinraj Zaki, AM Osterhaus, Ab Haagmans, Bart Gorbalenya, AE Snijder, EJ Fouchier, Ron |
author_sort |
van Boheemen, Sander |
title |
Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans |
title_short |
Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans |
title_full |
Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans |
title_fullStr |
Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans |
title_full_unstemmed |
Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans |
title_sort |
genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans |
publishDate |
2012 |
url |
https://pure.eur.nl/en/publications/79b27f15-cd4e-4e1e-aa13-c1de58095c87 https://doi.org/10.1128/mBio.00473-12 https://pure.eur.nl/ws/files/47480365/Repub_68528_O-A.pdf http://hdl.handle.net/1765/68528 |
genre |
Pipistrellus pipistrellus |
genre_facet |
Pipistrellus pipistrellus |
op_source |
van Boheemen , S , de Graaf , M , Lauber , C , Bestebroer , T , Victor , S , Zaki , AM , Osterhaus , A , Haagmans , B , Gorbalenya , AE , Snijder , EJ & Fouchier , R 2012 , ' Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans ' , mBio , vol. 3 , no. 6 . https://doi.org/10.1128/mBio.00473-12 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/mBio.00473-12 |
container_title |
mBio |
container_volume |
3 |
container_issue |
6 |
_version_ |
1766168844850692096 |