Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species
Inward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the tempera...
Published in: | Journal of Comparative Physiology B |
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Language: | English |
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Springer Verlag
2019
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Online Access: | http://elar.urfu.ru/handle/10995/90489 https://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85074697998 https://doi.org/10.1007/s00360-019-01241-9 |
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fturalfuniv:oai:elar.urfu.ru:10995/90489 2024-01-21T10:02:37+01:00 Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species Hassinen, M. Korajoki, H. Abramochkin, D. Krivosheya, P. Vornanen, M. 2019 application/pdf http://elar.urfu.ru/handle/10995/90489 https://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85074697998 https://doi.org/10.1007/s00360-019-01241-9 en eng Springer Verlag Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species / M. Hassinen, H. Korajoki, D. Abramochkin, P. Krivosheya, et al. . — DOI 10.1007/s00360-019-01241-9 // Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology. — 2019. — Vol. 6. — Iss. 189. — P. 735-749. 0174-1578 https://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf 1 efb96a94-12f2-4951-a473-0f182ceff855 http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85074697998 http://elar.urfu.ru/handle/10995/90489 doi:10.1007/s00360-019-01241-9 85074697998 000493668100001 info:eu-repo/semantics/openAccess cc-by Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology FISH HEART INWARD RECTIFIER POTASSIUM CHANNELS KIR2 PARALOGUES RT-QPCR THERMAL ACCLIMATION FRESH WATER INWARDLY RECTIFYING POTASSIUM CHANNEL ANIMAL AQUATIC SPECIES CLASSIFICATION ECOSYSTEM FISH GENE EXPRESSION REGULATION GENETICS HEART ATRIUM HEART VENTRICLE METABOLISM MOLECULAR CLONING PHYSIOLOGY SPECIES DIFFERENCE TEMPERATURE ANIMALS AQUATIC ORGANISMS CLONING MOLECULAR FISHES HEART ATRIA HEART VENTRICLES POTASSIUM CHANNELS INWARDLY RECTIFYING SPECIES SPECIFICITY Article info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 fturalfuniv https://doi.org/10.1007/s00360-019-01241-9 2023-12-26T01:59:15Z Inward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout (Salmo trutta fario), arctic char (Salvelinus alpinus), roach (Rutilus rutilus), perch (Perca fluviatilis) and pike (Esox lucius), and zebrafish (Danio rerio), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish. © 2019, The Author(s). Academy of Finland: 14955 Open access funding provided by University of Eastern Finland (UEF) including Kuopio University Hospital. The authors thank Anita Kervinen for her assistance with the fish. This work was supported by the Academy of Finland (grant number 14955). Article in Journal/Newspaper Arctic Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota Ural Federal University (URFU): ELAR Arctic Arctic Ocean Navaga ENVELOPE(162.106,162.106,59.229,59.229) Journal of Comparative Physiology B 189 6 735 749 |
institution |
Open Polar |
collection |
Ural Federal University (URFU): ELAR |
op_collection_id |
fturalfuniv |
language |
English |
topic |
FISH HEART INWARD RECTIFIER POTASSIUM CHANNELS KIR2 PARALOGUES RT-QPCR THERMAL ACCLIMATION FRESH WATER INWARDLY RECTIFYING POTASSIUM CHANNEL ANIMAL AQUATIC SPECIES CLASSIFICATION ECOSYSTEM FISH GENE EXPRESSION REGULATION GENETICS HEART ATRIUM HEART VENTRICLE METABOLISM MOLECULAR CLONING PHYSIOLOGY SPECIES DIFFERENCE TEMPERATURE ANIMALS AQUATIC ORGANISMS CLONING MOLECULAR FISHES HEART ATRIA HEART VENTRICLES POTASSIUM CHANNELS INWARDLY RECTIFYING SPECIES SPECIFICITY |
spellingShingle |
FISH HEART INWARD RECTIFIER POTASSIUM CHANNELS KIR2 PARALOGUES RT-QPCR THERMAL ACCLIMATION FRESH WATER INWARDLY RECTIFYING POTASSIUM CHANNEL ANIMAL AQUATIC SPECIES CLASSIFICATION ECOSYSTEM FISH GENE EXPRESSION REGULATION GENETICS HEART ATRIUM HEART VENTRICLE METABOLISM MOLECULAR CLONING PHYSIOLOGY SPECIES DIFFERENCE TEMPERATURE ANIMALS AQUATIC ORGANISMS CLONING MOLECULAR FISHES HEART ATRIA HEART VENTRICLES POTASSIUM CHANNELS INWARDLY RECTIFYING SPECIES SPECIFICITY Hassinen, M. Korajoki, H. Abramochkin, D. Krivosheya, P. Vornanen, M. Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
topic_facet |
FISH HEART INWARD RECTIFIER POTASSIUM CHANNELS KIR2 PARALOGUES RT-QPCR THERMAL ACCLIMATION FRESH WATER INWARDLY RECTIFYING POTASSIUM CHANNEL ANIMAL AQUATIC SPECIES CLASSIFICATION ECOSYSTEM FISH GENE EXPRESSION REGULATION GENETICS HEART ATRIUM HEART VENTRICLE METABOLISM MOLECULAR CLONING PHYSIOLOGY SPECIES DIFFERENCE TEMPERATURE ANIMALS AQUATIC ORGANISMS CLONING MOLECULAR FISHES HEART ATRIA HEART VENTRICLES POTASSIUM CHANNELS INWARDLY RECTIFYING SPECIES SPECIFICITY |
description |
Inward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout (Salmo trutta fario), arctic char (Salvelinus alpinus), roach (Rutilus rutilus), perch (Perca fluviatilis) and pike (Esox lucius), and zebrafish (Danio rerio), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish. © 2019, The Author(s). Academy of Finland: 14955 Open access funding provided by University of Eastern Finland (UEF) including Kuopio University Hospital. The authors thank Anita Kervinen for her assistance with the fish. This work was supported by the Academy of Finland (grant number 14955). |
format |
Article in Journal/Newspaper |
author |
Hassinen, M. Korajoki, H. Abramochkin, D. Krivosheya, P. Vornanen, M. |
author_facet |
Hassinen, M. Korajoki, H. Abramochkin, D. Krivosheya, P. Vornanen, M. |
author_sort |
Hassinen, M. |
title |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_short |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_full |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_fullStr |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_full_unstemmed |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_sort |
transcript expression of inward rectifier potassium channels of kir2 subfamily in arctic marine and freshwater fish species |
publisher |
Springer Verlag |
publishDate |
2019 |
url |
http://elar.urfu.ru/handle/10995/90489 https://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85074697998 https://doi.org/10.1007/s00360-019-01241-9 |
long_lat |
ENVELOPE(162.106,162.106,59.229,59.229) |
geographic |
Arctic Arctic Ocean Navaga |
geographic_facet |
Arctic Arctic Ocean Navaga |
genre |
Arctic Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota |
genre_facet |
Arctic Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota |
op_source |
Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology |
op_relation |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species / M. Hassinen, H. Korajoki, D. Abramochkin, P. Krivosheya, et al. . — DOI 10.1007/s00360-019-01241-9 // Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology. — 2019. — Vol. 6. — Iss. 189. — P. 735-749. 0174-1578 https://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf 1 efb96a94-12f2-4951-a473-0f182ceff855 http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85074697998 http://elar.urfu.ru/handle/10995/90489 doi:10.1007/s00360-019-01241-9 85074697998 000493668100001 |
op_rights |
info:eu-repo/semantics/openAccess cc-by |
op_doi |
https://doi.org/10.1007/s00360-019-01241-9 |
container_title |
Journal of Comparative Physiology B |
container_volume |
189 |
container_issue |
6 |
container_start_page |
735 |
op_container_end_page |
749 |
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1788692898080882688 |