Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni
The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N-2 fixation poses a challenge due to their undefined growth requirements and the...
Published in: | Science Advances |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Uppsala universitet, Mikrobiologi och immunologi
2024
|
Subjects: | |
Online Access: | http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-537019 https://doi.org/10.1126/sciadv.adn1476 |
id |
ftuppsalauniv:oai:DiVA.org:uu-537019 |
---|---|
record_format |
openpolar |
spelling |
ftuppsalauniv:oai:DiVA.org:uu-537019 2024-10-06T13:46:12+00:00 Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-Batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-Marie Kantor, Ella Joy H. Comeau, Andre M. Langille, Morgan G. I. Jerlström-Hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. LaRoche, Julie 2024 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-537019 https://doi.org/10.1126/sciadv.adn1476 eng eng Uppsala universitet, Mikrobiologi och immunologi Dalhousie Univ, Dept Biol, Halifax, NS, Canada. Dalhousie Univ, Dept Biol, Halifax, NS, Canada.;Clark Univ, Dept Biol, Worcester, MA USA. Dalhousie Univ, Dept Biol, Halifax, NS, Canada.;Dalhousie Univ, Dept Oceanog, Halifax, NS, Canada.;DOTCAN Inst, Halifax, NS, Canada. Dalhousie Univ, Dept Biol, Halifax, NS, Canada.;Dalhousie Univ, Integrated Microbiome Resource IMR, Halifax, NS, Canada.;Dalhousie Univ, Dept Pharmacol, Halifax, NS, Canada. Dalhousie Univ, Integrated Microbiome Resource IMR, Halifax, NS, Canada.;Dalhousie Univ, Dept Pharmacol, Halifax, NS, Canada. Fisheries & Oceans Canada, Bedford Inst Oceanog, Halifax, NS, Canada. Univ Brest, CNRS, IRD, Ifremer,LEMAR, F-29280 Plouzane, France. Science Advances, 2024, 10:31, orcid:0000-0002-7992-7970 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-537019 doi:10.1126/sciadv.adn1476 PMID 39083619 ISI:001281585300001 info:eu-repo/semantics/openAccess Microbiology Mikrobiologi Article in journal info:eu-repo/semantics/article text 2024 ftuppsalauniv https://doi.org/10.1126/sciadv.adn1476 2024-09-18T00:02:09Z The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N-2 fixation poses a challenge due to their undefined growth requirements and the complex regulation of the nitrogenase enzyme. We isolated and characterized Candidatus Thalassolituus haligoni, a member of a widely distributed clade of HBD belonging to the Oceanospirillales. Analysis of its nifH gene via amplicon sequencing revealed the extensive distribution of Cand. T. haligoni across the Pacific, Atlantic, and Arctic Oceans. Pangenome analysis indicates that the isolate shares >99% identity with an uncultured metagenome-assembled genome called Arc-Gamma-03, recently recovered from the Arctic Ocean. Through combined genomic, proteomic, and physiological approaches, we confirmed that the isolate fixes N-2 gas. However, the mechanisms governing nitrogenase regulation in Cand. T. haligoni remain unclear. We propose Cand. T. haligoni as a globally distributed, cultured HBD model species within this understudied clade of Oceanospirillales. Article in Journal/Newspaper Arctic Arctic Ocean Uppsala University: Publications (DiVA) Arctic Arctic Ocean Pacific Science Advances 10 31 |
institution |
Open Polar |
collection |
Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Microbiology Mikrobiologi |
spellingShingle |
Microbiology Mikrobiologi Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-Batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-Marie Kantor, Ella Joy H. Comeau, Andre M. Langille, Morgan G. I. Jerlström-Hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. LaRoche, Julie Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
topic_facet |
Microbiology Mikrobiologi |
description |
The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N-2 fixation poses a challenge due to their undefined growth requirements and the complex regulation of the nitrogenase enzyme. We isolated and characterized Candidatus Thalassolituus haligoni, a member of a widely distributed clade of HBD belonging to the Oceanospirillales. Analysis of its nifH gene via amplicon sequencing revealed the extensive distribution of Cand. T. haligoni across the Pacific, Atlantic, and Arctic Oceans. Pangenome analysis indicates that the isolate shares >99% identity with an uncultured metagenome-assembled genome called Arc-Gamma-03, recently recovered from the Arctic Ocean. Through combined genomic, proteomic, and physiological approaches, we confirmed that the isolate fixes N-2 gas. However, the mechanisms governing nitrogenase regulation in Cand. T. haligoni remain unclear. We propose Cand. T. haligoni as a globally distributed, cultured HBD model species within this understudied clade of Oceanospirillales. |
format |
Article in Journal/Newspaper |
author |
Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-Batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-Marie Kantor, Ella Joy H. Comeau, Andre M. Langille, Morgan G. I. Jerlström-Hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. LaRoche, Julie |
author_facet |
Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-Batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-Marie Kantor, Ella Joy H. Comeau, Andre M. Langille, Morgan G. I. Jerlström-Hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. LaRoche, Julie |
author_sort |
Rose, Sonja A. |
title |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_short |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_full |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_fullStr |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_full_unstemmed |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_sort |
nitrogen fixation in the widely distributed marine ãžâ³-proteobacterial diazotroph candidatus thalassolituus haligoni |
publisher |
Uppsala universitet, Mikrobiologi och immunologi |
publishDate |
2024 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-537019 https://doi.org/10.1126/sciadv.adn1476 |
geographic |
Arctic Arctic Ocean Pacific |
geographic_facet |
Arctic Arctic Ocean Pacific |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_relation |
Science Advances, 2024, 10:31, orcid:0000-0002-7992-7970 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-537019 doi:10.1126/sciadv.adn1476 PMID 39083619 ISI:001281585300001 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1126/sciadv.adn1476 |
container_title |
Science Advances |
container_volume |
10 |
container_issue |
31 |
_version_ |
1812174511751036928 |