Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures
Mining activities are increasingly recognized for contributing to nitrogen (N) pollution and possibly also to emissions of the greenhouse gas nitrous oxide (N2O) due to undetonated, N-based explosives. A woodchip denitrifying bioreactor, installed to treat nitrate-rich leachate from waste rock dum...
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Uppsala universitet, Luft-, vatten- och landskapslära
2024
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ftuppsalauniv:oai:DiVA.org:uu-526711 2024-05-12T08:08:57+00:00 Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures Hellman, Maria Juhanson, Jaanis Wallnäs, Felicia Herbert, Roger B. Hallin, Sara 2024 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-526711 https://doi.org/10.1016/j.jenvman.2024.120607 eng eng Uppsala universitet, Luft-, vatten- och landskapslära Sveriges lantbruksuniversitet (SLU) Journal of Environmental Management, 0301-4797, 2024, 356, orcid:0000-0002-0616-1126 orcid:0000-0003-3799-2819 orcid:0000-0002-7561-757x orcid:0000-0002-9069-9024 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-526711 doi:10.1016/j.jenvman.2024.120607 info:eu-repo/semantics/openAccess Denitrifying bioreactor Woodchips Denitrification DNRA N2O Microbial community Water Engineering Vattenteknik Microbiology Mikrobiologi Article in journal info:eu-repo/semantics/article text 2024 ftuppsalauniv https://doi.org/10.1016/j.jenvman.2024.120607 2024-04-17T14:07:23Z Mining activities are increasingly recognized for contributing to nitrogen (N) pollution and possibly also to emissions of the greenhouse gas nitrous oxide (N2O) due to undetonated, N-based explosives. A woodchip denitrifying bioreactor, installed to treat nitrate-rich leachate from waste rock dumps in northern Sweden, was monitored for two years to determine the spatial and temporal distribution of microbial communities, including the genetic potential for different N transformation processes, in pore water and woodchips and how this related to reactor N removal capacity. About 80 and 65 % of the nitrate was removed during the first and second operational year, respectively. There was a succession in the microbial community over time and in space along the reactor length in both pore water and woodchips, which was reflected in reactor performance. Nitrate ammonification likely had minimal impact on N removal efficiency due to the low production of ammonium and low abundance of the key gene nrfA in ammonifiers. Nitrite and N2O were formed in the bioreactor and released in the effluent water, although direct N2O emissions from the surface was low. That these unwanted reactive N species were produced at different times and locations in the reactor indicate that the denitrification pathway was temporally as well as spatially separated along the reactor length. We conclude that the succession of microbial communities in woodchip denitrifying bioreactors treating mining water develops slowly at low tem-perature, which impacts reactor performance.   Article in Journal/Newspaper Northern Sweden Uppsala University: Publications (DiVA) Journal of Environmental Management 356 120607 |
institution |
Open Polar |
collection |
Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Denitrifying bioreactor Woodchips Denitrification DNRA N2O Microbial community Water Engineering Vattenteknik Microbiology Mikrobiologi |
spellingShingle |
Denitrifying bioreactor Woodchips Denitrification DNRA N2O Microbial community Water Engineering Vattenteknik Microbiology Mikrobiologi Hellman, Maria Juhanson, Jaanis Wallnäs, Felicia Herbert, Roger B. Hallin, Sara Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
topic_facet |
Denitrifying bioreactor Woodchips Denitrification DNRA N2O Microbial community Water Engineering Vattenteknik Microbiology Mikrobiologi |
description |
Mining activities are increasingly recognized for contributing to nitrogen (N) pollution and possibly also to emissions of the greenhouse gas nitrous oxide (N2O) due to undetonated, N-based explosives. A woodchip denitrifying bioreactor, installed to treat nitrate-rich leachate from waste rock dumps in northern Sweden, was monitored for two years to determine the spatial and temporal distribution of microbial communities, including the genetic potential for different N transformation processes, in pore water and woodchips and how this related to reactor N removal capacity. About 80 and 65 % of the nitrate was removed during the first and second operational year, respectively. There was a succession in the microbial community over time and in space along the reactor length in both pore water and woodchips, which was reflected in reactor performance. Nitrate ammonification likely had minimal impact on N removal efficiency due to the low production of ammonium and low abundance of the key gene nrfA in ammonifiers. Nitrite and N2O were formed in the bioreactor and released in the effluent water, although direct N2O emissions from the surface was low. That these unwanted reactive N species were produced at different times and locations in the reactor indicate that the denitrification pathway was temporally as well as spatially separated along the reactor length. We conclude that the succession of microbial communities in woodchip denitrifying bioreactors treating mining water develops slowly at low tem-perature, which impacts reactor performance.   |
format |
Article in Journal/Newspaper |
author |
Hellman, Maria Juhanson, Jaanis Wallnäs, Felicia Herbert, Roger B. Hallin, Sara |
author_facet |
Hellman, Maria Juhanson, Jaanis Wallnäs, Felicia Herbert, Roger B. Hallin, Sara |
author_sort |
Hellman, Maria |
title |
Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
title_short |
Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
title_full |
Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
title_fullStr |
Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
title_full_unstemmed |
Microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
title_sort |
microbial succession and denitrifying woodchip bioreactor performance at low water temperatures |
publisher |
Uppsala universitet, Luft-, vatten- och landskapslära |
publishDate |
2024 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-526711 https://doi.org/10.1016/j.jenvman.2024.120607 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
Journal of Environmental Management, 0301-4797, 2024, 356, orcid:0000-0002-0616-1126 orcid:0000-0003-3799-2819 orcid:0000-0002-7561-757x orcid:0000-0002-9069-9024 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-526711 doi:10.1016/j.jenvman.2024.120607 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.jenvman.2024.120607 |
container_title |
Journal of Environmental Management |
container_volume |
356 |
container_start_page |
120607 |
_version_ |
1798852091904524288 |