Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus)
Background Substantial variation in male fertility is regularly observed in farmed Arctic charr. However, detailed investigations of its fluctuation during a reproductive season and across years are lacking. Furthermore, information about the effect of underlying genetic factors influencing sperm qu...
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Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi
2023
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ftuppsalauniv:oai:DiVA.org:uu-518755 2024-02-11T09:59:50+01:00 Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) Kurta, Khrystyna Jeuthe, Henrik Naboulsi, Rakan de Koning, Dirk-Jan Palaiokostas, Christos 2023 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-518755 https://doi.org/10.1186/s12864-023-09614-9 eng eng Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Box 7090, S-75007 Uppsala, Sweden. Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Box 7090, S-75007 Uppsala, Sweden.;Aquaculture Ctr North, Avagen 17, S-84461 Kalarne, Sweden. Karolinska Inst, Dept Womens & Childrens Hlth, Tomtebodavagen 18A, S-17177 Stockholm, Sweden. BMC Genomics, 2023, 24:1, http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-518755 doi:10.1186/s12864-023-09614-9 PMID 37667174 ISI:001116247700002 info:eu-repo/semantics/openAccess Arctic charr Sperm concentration Motility Season Age Whole-genome sequencing Fish and Aquacultural Science Fisk- och akvakulturforskning Article in journal info:eu-repo/semantics/article text 2023 ftuppsalauniv https://doi.org/10.1186/s12864-023-09614-9 2024-01-17T23:33:29Z Background Substantial variation in male fertility is regularly observed in farmed Arctic charr. However, detailed investigations of its fluctuation during a reproductive season and across years are lacking. Furthermore, information about the effect of underlying genetic factors influencing sperm quality is scarce. The current study focused on seasonal and age-related factors that may affect sperm quality characteristics in males reared in natural and delayed photoperiods. Animals were sampled three times for two consecutive years, and sperm quality parameters were recorded using a computer-assisted sperm analysis (CASA) system. Thereafter, high-throughput sequencing technologies were applied, aiming to identify genomic regions related to the variation of sperm quality throughout the reproductive season.Results An across-season variation in the recorded sperm quality parameters was evident. Overall, 29% and 42% of males from the natural and delayed spawning groups had a highly variable total progressive motility. Males at four years of age showed significantly higher sperm motility and velocities during the early October and November recordings compared to the following year when the same animals were five years of age. On the other hand, the opposite was observed regarding sperm concentration during the last sampling. A genome-wide F-ST scan detected SNP differentiation among males with high and low variability in total progressive motility (PM) on eight chromosomes (F-ST > 0.17), Genome wide windows with the highest F-ST contained SNPs in proximity (within 250 kb up- and downstream distance) to 16 genes with sperm quality biological functions in mammalian species.Conclusion Our findings provide a detailed view of seasonal, age-related, and genetic effects on sperm quality and can be used to guide decisions on broodstock selection and hatchery management. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Uppsala University: Publications (DiVA) Arctic BMC Genomics 24 1 |
institution |
Open Polar |
collection |
Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Arctic charr Sperm concentration Motility Season Age Whole-genome sequencing Fish and Aquacultural Science Fisk- och akvakulturforskning |
spellingShingle |
Arctic charr Sperm concentration Motility Season Age Whole-genome sequencing Fish and Aquacultural Science Fisk- och akvakulturforskning Kurta, Khrystyna Jeuthe, Henrik Naboulsi, Rakan de Koning, Dirk-Jan Palaiokostas, Christos Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) |
topic_facet |
Arctic charr Sperm concentration Motility Season Age Whole-genome sequencing Fish and Aquacultural Science Fisk- och akvakulturforskning |
description |
Background Substantial variation in male fertility is regularly observed in farmed Arctic charr. However, detailed investigations of its fluctuation during a reproductive season and across years are lacking. Furthermore, information about the effect of underlying genetic factors influencing sperm quality is scarce. The current study focused on seasonal and age-related factors that may affect sperm quality characteristics in males reared in natural and delayed photoperiods. Animals were sampled three times for two consecutive years, and sperm quality parameters were recorded using a computer-assisted sperm analysis (CASA) system. Thereafter, high-throughput sequencing technologies were applied, aiming to identify genomic regions related to the variation of sperm quality throughout the reproductive season.Results An across-season variation in the recorded sperm quality parameters was evident. Overall, 29% and 42% of males from the natural and delayed spawning groups had a highly variable total progressive motility. Males at four years of age showed significantly higher sperm motility and velocities during the early October and November recordings compared to the following year when the same animals were five years of age. On the other hand, the opposite was observed regarding sperm concentration during the last sampling. A genome-wide F-ST scan detected SNP differentiation among males with high and low variability in total progressive motility (PM) on eight chromosomes (F-ST > 0.17), Genome wide windows with the highest F-ST contained SNPs in proximity (within 250 kb up- and downstream distance) to 16 genes with sperm quality biological functions in mammalian species.Conclusion Our findings provide a detailed view of seasonal, age-related, and genetic effects on sperm quality and can be used to guide decisions on broodstock selection and hatchery management. |
format |
Article in Journal/Newspaper |
author |
Kurta, Khrystyna Jeuthe, Henrik Naboulsi, Rakan de Koning, Dirk-Jan Palaiokostas, Christos |
author_facet |
Kurta, Khrystyna Jeuthe, Henrik Naboulsi, Rakan de Koning, Dirk-Jan Palaiokostas, Christos |
author_sort |
Kurta, Khrystyna |
title |
Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) |
title_short |
Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) |
title_full |
Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) |
title_fullStr |
Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) |
title_full_unstemmed |
Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus) |
title_sort |
seasonal and age-related changes in sperm quality of farmed arctic charr (salvelinus alpinus) |
publisher |
Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi |
publishDate |
2023 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-518755 https://doi.org/10.1186/s12864-023-09614-9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_relation |
BMC Genomics, 2023, 24:1, http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-518755 doi:10.1186/s12864-023-09614-9 PMID 37667174 ISI:001116247700002 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12864-023-09614-9 |
container_title |
BMC Genomics |
container_volume |
24 |
container_issue |
1 |
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1790595578895269888 |