Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly
Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore arc...
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Uppsala universitet, Limnologi
2020
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Online Access: | http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-439530 https://doi.org/10.1111/1462-2920.15058 |
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ftuppsalauniv:oai:DiVA.org:uu-439530 2023-05-15T15:07:14+02:00 Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander 2020 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-439530 https://doi.org/10.1111/1462-2920.15058 eng eng Uppsala universitet, Limnologi Univ Jyväskylä, Dept Biol & Environm Sci, POB 35, Jyväskylä 40014, Finland Univ Oslo, Sect Aquat Biol & Toxicol, Ctr Biogeochem Anthropocene, Dept Biosci, Blindernv 31, N-0371 Oslo, Norway Univ Gothenburg, Dept Biol & Environm Sci, Box 463, S-40530 Gothenburg, Sweden; Tech Univ Munich, Chair Urban Water Syst Engn, Coulombwall 3, D-85748 Garching, Germany Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, SLU, Box 7050, S-75007 Uppsala, Sweden Swedish Univ Agr Sci, Dept Forest Mycol & Plant Pathol, Sci Life Lab, Almas Alle 5, S-75007 Uppsala, Sweden Univ Oslo, Sect Aquat Biol & Toxicol, Ctr Biogeochem Anthropocene, Dept Biosci, Blindernv 31, N-0371 Oslo, Norway; eDNA Solut AB, Björkåsgatan 16, S-43131 Mölndal, Sweden Environmental Microbiology, 1462-2912, 2020, 22:8, s. 3158-3171 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-439530 doi:10.1111/1462-2920.15058 PMID 32372550 ISI:000537306500001 info:eu-repo/semantics/openAccess Microbiology Mikrobiologi Ecology Ekologi Article in journal info:eu-repo/semantics/article text 2020 ftuppsalauniv https://doi.org/10.1111/1462-2920.15058 2023-02-23T21:55:09Z Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large-scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea-specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co-occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake-specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia-oxidizing archaea were frequently detected across the lakes. Archaea-specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes. Article in Journal/Newspaper Arctic Uppsala University: Publications (DiVA) Arctic Environmental Microbiology 22 8 3158 3171 |
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Open Polar |
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Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Microbiology Mikrobiologi Ecology Ekologi |
spellingShingle |
Microbiology Mikrobiologi Ecology Ekologi Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
topic_facet |
Microbiology Mikrobiologi Ecology Ekologi |
description |
Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large-scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea-specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co-occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake-specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia-oxidizing archaea were frequently detected across the lakes. Archaea-specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes. |
format |
Article in Journal/Newspaper |
author |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander |
author_facet |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander |
author_sort |
Juottonen, Heli |
title |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_short |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_full |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_fullStr |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_full_unstemmed |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_sort |
archaea in boreal swedish lakes are diverse, dominated by woesearchaeota and follow deterministic community assembly |
publisher |
Uppsala universitet, Limnologi |
publishDate |
2020 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-439530 https://doi.org/10.1111/1462-2920.15058 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
Environmental Microbiology, 1462-2912, 2020, 22:8, s. 3158-3171 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-439530 doi:10.1111/1462-2920.15058 PMID 32372550 ISI:000537306500001 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/1462-2920.15058 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
8 |
container_start_page |
3158 |
op_container_end_page |
3171 |
_version_ |
1766338782644142080 |