Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
Background: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content...
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Uppsala universitet, Geriatrik
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ftuppsalauniv:oai:DiVA.org:uu-378996 2024-02-11T10:07:11+01:00 Urea dilution of serum for reproducible anti-HSV1 IgG avidity index Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lovheim, Hugo Elgh, Fredrik 2019 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-378996 https://doi.org/10.1186/s12879-019-3769-x eng eng Uppsala universitet, Geriatrik Umeå Univ, Dept Clin Microbiol, Virol, Umeå, Sweden Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden Umeå Univ, Dept Clin Microbiol, Umeå, Sweden Tampere Univ Hosp, Tampere, Finland Umeå Univ, Community Med & Rehabil, Geriatr Med, Umeå, Sweden BMC Infectious Diseases, 2019, 19, orcid:0000-0003-3232-6227 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-378996 doi:10.1186/s12879-019-3769-x PMID 30764767 ISI:000459030400003 info:eu-repo/semantics/openAccess Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin Article in journal info:eu-repo/semantics/article text 2019 ftuppsalauniv https://doi.org/10.1186/s12879-019-3769-x 2024-01-17T23:33:33Z Background: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Methods: Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. Results: AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900–0.979) and 0.989 (95% CI 0.969–0.996) in the biobank and clinical samples, respectively. Conclusion: The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts. Article in Journal/Newspaper Northern Sweden Uppsala University: Publications (DiVA) BMC Infectious Diseases 19 1 |
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Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin |
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Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lovheim, Hugo Elgh, Fredrik Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
topic_facet |
Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin |
description |
Background: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Methods: Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. Results: AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900–0.979) and 0.989 (95% CI 0.969–0.996) in the biobank and clinical samples, respectively. Conclusion: The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts. |
format |
Article in Journal/Newspaper |
author |
Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lovheim, Hugo Elgh, Fredrik |
author_facet |
Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lovheim, Hugo Elgh, Fredrik |
author_sort |
Olsson, Jan |
title |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_short |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_full |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_fullStr |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_full_unstemmed |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_sort |
urea dilution of serum for reproducible anti-hsv1 igg avidity index |
publisher |
Uppsala universitet, Geriatrik |
publishDate |
2019 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-378996 https://doi.org/10.1186/s12879-019-3769-x |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
BMC Infectious Diseases, 2019, 19, orcid:0000-0003-3232-6227 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-378996 doi:10.1186/s12879-019-3769-x PMID 30764767 ISI:000459030400003 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12879-019-3769-x |
container_title |
BMC Infectious Diseases |
container_volume |
19 |
container_issue |
1 |
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1790605336964497408 |