Development of transcriptome genetic markers for the great snipe (Gallinago media)

We sequenced the transcriptomes of 14 great snipe (Gallinago media) males from the Gavalia study population (central Norway) using Roche 454 technology. The assembled transcriptome sequences (RNA-Seq) was used to identify 140 microsatellite repeat sequences with sufficient flanking sequence informat...

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Bibliographic Details
Published in:Conservation Genetics Resources
Main Authors: Ekblom, Robert, Biao, Wang
Format: Article in Journal/Newspaper
Language:English
Published: Uppsala universitet, Evolutionsbiologi 2017
Subjects:
Shore bird
Molecular markers
454 sequencing
Microsatellites
SNPs
Genetics
Genetik
Norway
Gallinago media
great snipe
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-342664
https://doi.org/10.1007/s12686-017-0746-9
Description
Summary:We sequenced the transcriptomes of 14 great snipe (Gallinago media) males from the Gavalia study population (central Norway) using Roche 454 technology. The assembled transcriptome sequences (RNA-Seq) was used to identify 140 microsatellite repeat sequences with sufficient flanking sequence information for primer design. In addition several 1000 single nucleotide polymorphisms in the transcriptome were identified, and a small subset of these were verified by independent genotyping.

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