The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin
Mine water effluent contains high levels of nitrogen due to residues from undetonated ammonium- nitrate based explosives. Excess nitrogen in aquatic ecosystems can cause eutrophication. Within a mining area, tailings and clarification ponds have the potential to reduce nitrogen levels by biological...
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Sveriges Lantbruksuniversitet, Instutionen för mikrobiologi
2014
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ftuppsalauniv:oai:DiVA.org:uu-230567 2023-05-15T17:04:10+02:00 The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin Lindberg, Hanna 2014 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230567 eng eng Sveriges Lantbruksuniversitet, Instutionen för mikrobiologi UPTEC W, 1401-5765 14021 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230567 info:eu-repo/semantics/openAccess Denitrification mine effluent nitrogen external carbon sediment Student thesis info:eu-repo/semantics/bachelorThesis text 2014 ftuppsalauniv 2023-02-23T21:40:11Z Mine water effluent contains high levels of nitrogen due to residues from undetonated ammonium- nitrate based explosives. Excess nitrogen in aquatic ecosystems can cause eutrophication. Within a mining area, tailings and clarification ponds have the potential to reduce nitrogen levels by biological uptake of nitrogen into growing algae and denitrification in pond sediments. A previous study at the LKAB Kiruna mine investigated the potential nitrogen removal within the tailings and clarification ponds. The study showed that about 1-10 tonnes of nitrogen were removed each year, and that the removal by denitrification was limited by carbon. The aim of this master thesis was to investigate if additions of different carbon compounds could improve the denitrification in sediment from the clarification pond at the LKAB Kiruna mine site. It was also of interest to see if the composition of the edogenous microbial community involved in nitrogen reduction changed after the treatments. Samples of sediment and pond water were collected in January 2014 and a laboratory experiment was set up where sediment and water was incubated with carbon additions under anoxic conditions. Three different carbon sources were tested: sodium acetate, hydroxyethyl cellulose and green algae. Pond water without additional carbon was used as a control. The sediment was incubated eight weeks at 20 °C with weekly water exchange and carbon addition. The removed water was analyzed to determine the amount of nitrogen removed. At start and after ending the incubation, potential denitrification in the sediment was determined with an enzymatic assay and the size of the genetic potential of nitrogen reduction was determined. At start, the enzymatic assay showed that the potential denitrification rate in the sediment of the clarification pond at the LKAB Kiruna mine was not immediately enhanced by addition of carbon. However, during the incubation the removal of nitrate was enhanced by external carbon sources. Algae were a good carbon source, since the ... Bachelor Thesis Kiruna Uppsala University: Publications (DiVA) Kiruna |
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Uppsala University: Publications (DiVA) |
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ftuppsalauniv |
language |
English |
topic |
Denitrification mine effluent nitrogen external carbon sediment |
spellingShingle |
Denitrification mine effluent nitrogen external carbon sediment Lindberg, Hanna The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
topic_facet |
Denitrification mine effluent nitrogen external carbon sediment |
description |
Mine water effluent contains high levels of nitrogen due to residues from undetonated ammonium- nitrate based explosives. Excess nitrogen in aquatic ecosystems can cause eutrophication. Within a mining area, tailings and clarification ponds have the potential to reduce nitrogen levels by biological uptake of nitrogen into growing algae and denitrification in pond sediments. A previous study at the LKAB Kiruna mine investigated the potential nitrogen removal within the tailings and clarification ponds. The study showed that about 1-10 tonnes of nitrogen were removed each year, and that the removal by denitrification was limited by carbon. The aim of this master thesis was to investigate if additions of different carbon compounds could improve the denitrification in sediment from the clarification pond at the LKAB Kiruna mine site. It was also of interest to see if the composition of the edogenous microbial community involved in nitrogen reduction changed after the treatments. Samples of sediment and pond water were collected in January 2014 and a laboratory experiment was set up where sediment and water was incubated with carbon additions under anoxic conditions. Three different carbon sources were tested: sodium acetate, hydroxyethyl cellulose and green algae. Pond water without additional carbon was used as a control. The sediment was incubated eight weeks at 20 °C with weekly water exchange and carbon addition. The removed water was analyzed to determine the amount of nitrogen removed. At start and after ending the incubation, potential denitrification in the sediment was determined with an enzymatic assay and the size of the genetic potential of nitrogen reduction was determined. At start, the enzymatic assay showed that the potential denitrification rate in the sediment of the clarification pond at the LKAB Kiruna mine was not immediately enhanced by addition of carbon. However, during the incubation the removal of nitrate was enhanced by external carbon sources. Algae were a good carbon source, since the ... |
format |
Bachelor Thesis |
author |
Lindberg, Hanna |
author_facet |
Lindberg, Hanna |
author_sort |
Lindberg, Hanna |
title |
The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
title_short |
The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
title_full |
The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
title_fullStr |
The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
title_full_unstemmed |
The effect of different carbon sources on reduction of nitrate in effluent from the mining industry : Olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
title_sort |
effect of different carbon sources on reduction of nitrate in effluent from the mining industry : olika kolkällors inverkan på reduktion av nitrat i processvatten från gruvindustrin |
publisher |
Sveriges Lantbruksuniversitet, Instutionen för mikrobiologi |
publishDate |
2014 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230567 |
geographic |
Kiruna |
geographic_facet |
Kiruna |
genre |
Kiruna |
genre_facet |
Kiruna |
op_relation |
UPTEC W, 1401-5765 14021 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230567 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766058212480516096 |