Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica
Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adeli...
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Uppsala universitet, Infektionssjukdomar
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ftuppsalauniv:oai:DiVA.org:uu-230531 2023-05-15T14:03:20+02:00 Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica Hurt, Aeron C. Vijaykrishna, Dhanasekaran Butler, Jeffrey Baas, Chantal Maurer-Stroh, Sebastian Carolina Silva-de-la-Fuente, M. Medina-Vogel, Gonzalo Olsen, Björn Kelso, Anne Barr, Ian G. Gonzalez-Acuna, Daniel 2014 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230531 https://doi.org/10.1128/mBio.01098-14 eng eng Uppsala universitet, Infektionssjukdomar mBio, 2161-2129, 2014, 5:3, s. e01098-14- http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230531 doi:10.1128/mBio.01098-14 ISI:000338875900032 info:eu-repo/semantics/openAccess Microbiology in the medical area Mikrobiologi inom det medicinska området Article in journal info:eu-repo/semantics/article text 2014 ftuppsalauniv https://doi.org/10.1128/mBio.01098-14 2023-02-23T21:46:14Z Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adelie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra-and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment. IMPORTANCE Avian influenza viruses (AIVs) are typically maintained and spread by migratory birds, resulting in the existence of distinctly different viruses around the world. However, AIVs have not previously been detected in Antarctica. In this study, we characterized H11N2 viruses sampled from Adelie penguins from ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Uppsala University: Publications (DiVA) Antarctic The Antarctic mBio 5 3 |
institution |
Open Polar |
collection |
Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Microbiology in the medical area Mikrobiologi inom det medicinska området |
spellingShingle |
Microbiology in the medical area Mikrobiologi inom det medicinska området Hurt, Aeron C. Vijaykrishna, Dhanasekaran Butler, Jeffrey Baas, Chantal Maurer-Stroh, Sebastian Carolina Silva-de-la-Fuente, M. Medina-Vogel, Gonzalo Olsen, Björn Kelso, Anne Barr, Ian G. Gonzalez-Acuna, Daniel Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica |
topic_facet |
Microbiology in the medical area Mikrobiologi inom det medicinska området |
description |
Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adelie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra-and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment. IMPORTANCE Avian influenza viruses (AIVs) are typically maintained and spread by migratory birds, resulting in the existence of distinctly different viruses around the world. However, AIVs have not previously been detected in Antarctica. In this study, we characterized H11N2 viruses sampled from Adelie penguins from ... |
format |
Article in Journal/Newspaper |
author |
Hurt, Aeron C. Vijaykrishna, Dhanasekaran Butler, Jeffrey Baas, Chantal Maurer-Stroh, Sebastian Carolina Silva-de-la-Fuente, M. Medina-Vogel, Gonzalo Olsen, Björn Kelso, Anne Barr, Ian G. Gonzalez-Acuna, Daniel |
author_facet |
Hurt, Aeron C. Vijaykrishna, Dhanasekaran Butler, Jeffrey Baas, Chantal Maurer-Stroh, Sebastian Carolina Silva-de-la-Fuente, M. Medina-Vogel, Gonzalo Olsen, Björn Kelso, Anne Barr, Ian G. Gonzalez-Acuna, Daniel |
author_sort |
Hurt, Aeron C. |
title |
Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica |
title_short |
Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica |
title_full |
Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica |
title_fullStr |
Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica |
title_full_unstemmed |
Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica |
title_sort |
detection of evolutionarily distinct avian influenza a viruses in antarctica |
publisher |
Uppsala universitet, Infektionssjukdomar |
publishDate |
2014 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230531 https://doi.org/10.1128/mBio.01098-14 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
mBio, 2161-2129, 2014, 5:3, s. e01098-14- http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-230531 doi:10.1128/mBio.01098-14 ISI:000338875900032 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/mBio.01098-14 |
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mBio |
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5 |
container_issue |
3 |
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1766273965805797376 |