Protein engineering for development of new hydrolytic biocatalysts
Hydrolytic enzymes play important roles as biocatalysts in chemical synthesis. The chemical versatility and structurally sturdy features of Candida antarctica lipase B has placed this enzyme as a common utensil in the synthetic tool-box. In addition to catalyzing acyl transfer reactions, a number of...
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Uppsala universitet, Institutionen för kemi - BMC
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ftuppsalauniv:oai:DiVA.org:uu-221378 2023-05-15T13:52:43+02:00 Protein engineering for development of new hydrolytic biocatalysts Widersten, Mikael 2014 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-221378 https://doi.org/10.1016/j.cbpa.2014.03.015 eng eng Uppsala universitet, Institutionen för kemi - BMC Current opinion in chemical biology, 1367-5931, 2014, 21, s. 42-47 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-221378 doi:10.1016/j.cbpa.2014.03.015 ISI:000341900100008 info:eu-repo/semantics/openAccess Biochemistry and Molecular Biology Biokemi och molekylärbiologi Article, review/survey info:eu-repo/semantics/article text 2014 ftuppsalauniv https://doi.org/10.1016/j.cbpa.2014.03.015 2023-02-23T21:45:21Z Hydrolytic enzymes play important roles as biocatalysts in chemical synthesis. The chemical versatility and structurally sturdy features of Candida antarctica lipase B has placed this enzyme as a common utensil in the synthetic tool-box. In addition to catalyzing acyl transfer reactions, a number of promiscuous activities have been described recently. Some of these new enzyme activities have been amplified by mutagenesis. Epoxide hydrolases are of interest due to their potential as catalysts in asymmetric synthesis. This current update discusses recent development in the engineering of lipases and epoxide hydrolases aiming to generate new biocatalysts with refined features as compared to the wild-type enzymes. Reported progress in improvements in reaction atom economy from dynamic kinetic resolution or enantioconvergence are also included. Article in Journal/Newspaper Antarc* Antarctica Uppsala University: Publications (DiVA) Current Opinion in Chemical Biology 21 42 47 |
institution |
Open Polar |
collection |
Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Biochemistry and Molecular Biology Biokemi och molekylärbiologi |
spellingShingle |
Biochemistry and Molecular Biology Biokemi och molekylärbiologi Widersten, Mikael Protein engineering for development of new hydrolytic biocatalysts |
topic_facet |
Biochemistry and Molecular Biology Biokemi och molekylärbiologi |
description |
Hydrolytic enzymes play important roles as biocatalysts in chemical synthesis. The chemical versatility and structurally sturdy features of Candida antarctica lipase B has placed this enzyme as a common utensil in the synthetic tool-box. In addition to catalyzing acyl transfer reactions, a number of promiscuous activities have been described recently. Some of these new enzyme activities have been amplified by mutagenesis. Epoxide hydrolases are of interest due to their potential as catalysts in asymmetric synthesis. This current update discusses recent development in the engineering of lipases and epoxide hydrolases aiming to generate new biocatalysts with refined features as compared to the wild-type enzymes. Reported progress in improvements in reaction atom economy from dynamic kinetic resolution or enantioconvergence are also included. |
format |
Article in Journal/Newspaper |
author |
Widersten, Mikael |
author_facet |
Widersten, Mikael |
author_sort |
Widersten, Mikael |
title |
Protein engineering for development of new hydrolytic biocatalysts |
title_short |
Protein engineering for development of new hydrolytic biocatalysts |
title_full |
Protein engineering for development of new hydrolytic biocatalysts |
title_fullStr |
Protein engineering for development of new hydrolytic biocatalysts |
title_full_unstemmed |
Protein engineering for development of new hydrolytic biocatalysts |
title_sort |
protein engineering for development of new hydrolytic biocatalysts |
publisher |
Uppsala universitet, Institutionen för kemi - BMC |
publishDate |
2014 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-221378 https://doi.org/10.1016/j.cbpa.2014.03.015 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Current opinion in chemical biology, 1367-5931, 2014, 21, s. 42-47 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-221378 doi:10.1016/j.cbpa.2014.03.015 ISI:000341900100008 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.cbpa.2014.03.015 |
container_title |
Current Opinion in Chemical Biology |
container_volume |
21 |
container_start_page |
42 |
op_container_end_page |
47 |
_version_ |
1766257193356623872 |