Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP)
The Agouti-like peptides including AgRP, ASIP and the teleost-specific A2 (ASIP2 and AgRP2) peptides have potent and diverse functional roles in feeding, pigmentation and background adaptation mechanisms. There are contradictory theories about the evolution of the Agouti-like peptide family as well...
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Uppsala universitet, Funktionell farmakologi
2012
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ftuppsalauniv:oai:DiVA.org:uu-179576 2023-05-15T13:30:41+02:00 Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) Västermark, Åke Krishnan, Arunkumar Houle, Michael E. Fredriksson, Robert Miguel Cerda-Reverter, Jose Schiöth, Helgi B. 2012 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-179576 https://doi.org/10.1371/journal.pone.0040982 eng eng Uppsala universitet, Funktionell farmakologi PLOS ONE, 2012, 7:7, s. e40982- http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-179576 doi:10.1371/journal.pone.0040982 ISI:000306466100103 info:eu-repo/semantics/openAccess Medical and Health Sciences Medicin och hälsovetenskap Article in journal info:eu-repo/semantics/article text 2012 ftuppsalauniv https://doi.org/10.1371/journal.pone.0040982 2023-02-23T21:55:58Z The Agouti-like peptides including AgRP, ASIP and the teleost-specific A2 (ASIP2 and AgRP2) peptides have potent and diverse functional roles in feeding, pigmentation and background adaptation mechanisms. There are contradictory theories about the evolution of the Agouti-like peptide family as well the nomenclature. Here we performed comprehensive mining and annotation of vertebrate Agouti-like sequences. We identified A2 sequences from salmon, trout, seabass, cod, cichlid, tilapia, gilt-headed sea bream, Antarctic toothfish, rainbow smelt, common carp, channel catfish and interestingly also in lobe-finned fish. Moreover, we surprisingly found eight novel homologues from the kingdom of arthropods and three from fungi, some sharing the characteristic C-x(6)-C-C motif which are present in the Agouti-like sequences, as well as approximate sequence length (130 amino acids), positioning of the motif sequence and sharing of exon-intron structures that are similar to the other Agouti-like peptides providing further support for the common origin of these sequences. Phylogenetic analysis shows that the AgRP sequences cluster basally in the tree, suggesting that these sequences split from a cluster containing both the ASIP and the A2 sequences. We also used a novel approach to determine the statistical evidence for synteny, a sinusoidal Hough transform pattern recognition technique. Our analysis shows that the teleost AgRP2 resides in a chromosomal region that has synteny with Hsa 8, but we found no convincing synteny between the regions that A2, AgRP and ASIP reside in, which would support that the Agouti-like peptides were formed by whole genome tetraplodization events. Here we suggest that the Agouti-like peptide genes were formed through classical subsequent gene duplications where the AgRP is the most distantly related to the three other members of that group, first splitting from a common ancestor to ASIP and A2, and then later the A2 split from ASIP followed by a split resulting in ASIP2 and AgRP2. Article in Journal/Newspaper Antarc* Antarctic Antarctic Toothfish Uppsala University: Publications (DiVA) Antarctic PLoS ONE 7 7 e40982 |
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Open Polar |
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Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Medical and Health Sciences Medicin och hälsovetenskap |
spellingShingle |
Medical and Health Sciences Medicin och hälsovetenskap Västermark, Åke Krishnan, Arunkumar Houle, Michael E. Fredriksson, Robert Miguel Cerda-Reverter, Jose Schiöth, Helgi B. Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) |
topic_facet |
Medical and Health Sciences Medicin och hälsovetenskap |
description |
The Agouti-like peptides including AgRP, ASIP and the teleost-specific A2 (ASIP2 and AgRP2) peptides have potent and diverse functional roles in feeding, pigmentation and background adaptation mechanisms. There are contradictory theories about the evolution of the Agouti-like peptide family as well the nomenclature. Here we performed comprehensive mining and annotation of vertebrate Agouti-like sequences. We identified A2 sequences from salmon, trout, seabass, cod, cichlid, tilapia, gilt-headed sea bream, Antarctic toothfish, rainbow smelt, common carp, channel catfish and interestingly also in lobe-finned fish. Moreover, we surprisingly found eight novel homologues from the kingdom of arthropods and three from fungi, some sharing the characteristic C-x(6)-C-C motif which are present in the Agouti-like sequences, as well as approximate sequence length (130 amino acids), positioning of the motif sequence and sharing of exon-intron structures that are similar to the other Agouti-like peptides providing further support for the common origin of these sequences. Phylogenetic analysis shows that the AgRP sequences cluster basally in the tree, suggesting that these sequences split from a cluster containing both the ASIP and the A2 sequences. We also used a novel approach to determine the statistical evidence for synteny, a sinusoidal Hough transform pattern recognition technique. Our analysis shows that the teleost AgRP2 resides in a chromosomal region that has synteny with Hsa 8, but we found no convincing synteny between the regions that A2, AgRP and ASIP reside in, which would support that the Agouti-like peptides were formed by whole genome tetraplodization events. Here we suggest that the Agouti-like peptide genes were formed through classical subsequent gene duplications where the AgRP is the most distantly related to the three other members of that group, first splitting from a common ancestor to ASIP and A2, and then later the A2 split from ASIP followed by a split resulting in ASIP2 and AgRP2. |
format |
Article in Journal/Newspaper |
author |
Västermark, Åke Krishnan, Arunkumar Houle, Michael E. Fredriksson, Robert Miguel Cerda-Reverter, Jose Schiöth, Helgi B. |
author_facet |
Västermark, Åke Krishnan, Arunkumar Houle, Michael E. Fredriksson, Robert Miguel Cerda-Reverter, Jose Schiöth, Helgi B. |
author_sort |
Västermark, Åke |
title |
Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) |
title_short |
Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) |
title_full |
Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) |
title_fullStr |
Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) |
title_full_unstemmed |
Identification of Distant Agouti-Like Sequences and Re-Evaluation of the Evolutionary History of the Agouti-Related Peptide (AgRP) |
title_sort |
identification of distant agouti-like sequences and re-evaluation of the evolutionary history of the agouti-related peptide (agrp) |
publisher |
Uppsala universitet, Funktionell farmakologi |
publishDate |
2012 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-179576 https://doi.org/10.1371/journal.pone.0040982 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Toothfish |
genre_facet |
Antarc* Antarctic Antarctic Toothfish |
op_relation |
PLOS ONE, 2012, 7:7, s. e40982- http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-179576 doi:10.1371/journal.pone.0040982 ISI:000306466100103 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0040982 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
7 |
container_start_page |
e40982 |
_version_ |
1766011438284931072 |