The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus
The bank vole (Microtus arvalis) develops glucose intolerance both when kept in captivity and in the wild state. Glucose intolerant bank voles kept in captivity exhibited polydipsia, polyuria, hyperglycemia, hyperinsulinemia, islet autoantibodies and a markedly changed islet structure resembling so–...
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Uppsala universitet, Institutionen för medicinsk cellbiologi
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ftuppsalauniv:oai:DiVA.org:uu-122715 2023-05-15T17:12:39+02:00 The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus Blixt, Martin 2010 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122715 eng eng Uppsala universitet, Institutionen för medicinsk cellbiologi Uppsala : Acta Universitatis Upsaliensis Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1651-6206 559 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122715 urn:isbn:978-91-554-7806-3 info:eu-repo/semantics/openAccess Bank vole pancreatic islets diabetes mellitus hydropic degeneration proinflammatory cytokine Diabetology Diabetologi Endocrinology Endokrinologi Doctoral thesis, comprehensive summary info:eu-repo/semantics/doctoralThesis text 2010 ftuppsalauniv 2023-02-23T21:43:55Z The bank vole (Microtus arvalis) develops glucose intolerance both when kept in captivity and in the wild state. Glucose intolerant bank voles kept in captivity exhibited polydipsia, polyuria, hyperglycemia, hyperinsulinemia, islet autoantibodies and a markedly changed islet structure resembling so–called hydropic degeneration. Islets showing hydropic degeneration have reduced β–cell mass. However, the relative islet size to total pancreas area was not changed. Pancreatic islet isolated from glucose intolerant bank voles had an altered islet function showing signs of being exposed to an increased functional demand on their β–cells. Also, islets from male bank voles seem more affected than the islets from females. Islets isolated from glucose tolerant male bank voles cultured for 5 days at 28 mM glucose did not reveal any change in insulin gene expression or insulin biosynthesis rate. However, islets from female bank voles displayed a glucose concentration dependent response. This suggests that there is gender difference in that, islets of female more easily than islets of males adapt to elevated glucose concentration. Furthermore, islets isolated from glucose tolerant males had reduced insulin gene expression after exposure to proinflammatory cytokines for 48 hrs. This effect seemed to be NO-independent since only a minor elevation of nitrite accumulation in the medium was seen, and the use of iNOS inhibitor could not counteract the cytokine effect. The observed response seen in bank vole islets upon exposure to various glucose concentrations or proinflammatory cytokines is similar to those seen in studies of human islets. The bank vole may therefore represent a novel animal model for the study of diabetes. An unresolved issue is the role of the Ljungan virus which is found in the bank vole colony. Bank voles developing glucose intolerance display features of both human type 1 and type 2 diabetes, where environmental factors seems to play an important role as determinant. Our findings suggest that bank voles ... Doctoral or Postdoctoral Thesis Microtus arvalis Uppsala University: Publications (DiVA) The Islets ENVELOPE(-55.448,-55.448,51.600,51.600) |
institution |
Open Polar |
collection |
Uppsala University: Publications (DiVA) |
op_collection_id |
ftuppsalauniv |
language |
English |
topic |
Bank vole pancreatic islets diabetes mellitus hydropic degeneration proinflammatory cytokine Diabetology Diabetologi Endocrinology Endokrinologi |
spellingShingle |
Bank vole pancreatic islets diabetes mellitus hydropic degeneration proinflammatory cytokine Diabetology Diabetologi Endocrinology Endokrinologi Blixt, Martin The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus |
topic_facet |
Bank vole pancreatic islets diabetes mellitus hydropic degeneration proinflammatory cytokine Diabetology Diabetologi Endocrinology Endokrinologi |
description |
The bank vole (Microtus arvalis) develops glucose intolerance both when kept in captivity and in the wild state. Glucose intolerant bank voles kept in captivity exhibited polydipsia, polyuria, hyperglycemia, hyperinsulinemia, islet autoantibodies and a markedly changed islet structure resembling so–called hydropic degeneration. Islets showing hydropic degeneration have reduced β–cell mass. However, the relative islet size to total pancreas area was not changed. Pancreatic islet isolated from glucose intolerant bank voles had an altered islet function showing signs of being exposed to an increased functional demand on their β–cells. Also, islets from male bank voles seem more affected than the islets from females. Islets isolated from glucose tolerant male bank voles cultured for 5 days at 28 mM glucose did not reveal any change in insulin gene expression or insulin biosynthesis rate. However, islets from female bank voles displayed a glucose concentration dependent response. This suggests that there is gender difference in that, islets of female more easily than islets of males adapt to elevated glucose concentration. Furthermore, islets isolated from glucose tolerant males had reduced insulin gene expression after exposure to proinflammatory cytokines for 48 hrs. This effect seemed to be NO-independent since only a minor elevation of nitrite accumulation in the medium was seen, and the use of iNOS inhibitor could not counteract the cytokine effect. The observed response seen in bank vole islets upon exposure to various glucose concentrations or proinflammatory cytokines is similar to those seen in studies of human islets. The bank vole may therefore represent a novel animal model for the study of diabetes. An unresolved issue is the role of the Ljungan virus which is found in the bank vole colony. Bank voles developing glucose intolerance display features of both human type 1 and type 2 diabetes, where environmental factors seems to play an important role as determinant. Our findings suggest that bank voles ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Blixt, Martin |
author_facet |
Blixt, Martin |
author_sort |
Blixt, Martin |
title |
The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus |
title_short |
The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus |
title_full |
The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus |
title_fullStr |
The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus |
title_full_unstemmed |
The bank vole (Myodes glareolus) – a novel animal model for the study of diabetes mellitus |
title_sort |
bank vole (myodes glareolus) – a novel animal model for the study of diabetes mellitus |
publisher |
Uppsala universitet, Institutionen för medicinsk cellbiologi |
publishDate |
2010 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122715 |
long_lat |
ENVELOPE(-55.448,-55.448,51.600,51.600) |
geographic |
The Islets |
geographic_facet |
The Islets |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1651-6206 559 http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122715 urn:isbn:978-91-554-7806-3 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766069441105231872 |