Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii
Biological interactions between various classes of pollutants are of great relevance for the Antarctic marine environment, where the naturally elevated bioavailability of metals like cadmium might indirectly influence sensitivity of endemic organisms toward other environmental pollutants, e.g. polyc...
Published in: | Aquatic Toxicology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2007
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Online Access: | http://hdl.handle.net/11566/53440 https://doi.org/10.1016/j.aquatox.2007.08.009 |
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ftupmarcheiris:oai:iris.univpm.it:11566/53440 2024-04-14T08:03:50+00:00 Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii BENEDETTI, MAURA MARTUCCIO G FATTORINI, DANIELE CANAPA, Adriana BARUCCA, MARCO NIGRO M REGOLI, Francesco Benedetti, Maura Martuccio, G Fattorini, Daniele Canapa, Adriana Barucca, Marco Nigro, M Regoli, Francesco 2007 http://hdl.handle.net/11566/53440 https://doi.org/10.1016/j.aquatox.2007.08.009 eng eng info:eu-repo/semantics/altIdentifier/pmid/17923160 info:eu-repo/semantics/altIdentifier/wos/WOS:000251639100001 volume:85 issue:3 firstpage:167 lastpage:175 numberofpages:9 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11566/53440 doi:10.1016/j.aquatox.2007.08.009 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-35448996882 Antarctica Bioaccumulation Cytochrome P450 Interactions Oxidative stress Polycyclic aromatic hydrocarbons Trace metals Trematomus bernacchii info:eu-repo/semantics/article 2007 ftupmarcheiris https://doi.org/10.1016/j.aquatox.2007.08.009 2024-03-21T18:29:50Z Biological interactions between various classes of pollutants are of great relevance for the Antarctic marine environment, where the naturally elevated bioavailability of metals like cadmium might indirectly influence sensitivity of endemic organisms toward other environmental pollutants, e.g. polycyclic aromatic hydrocarbons (PAHs). To further investigate reciprocal effects of different chemicals, the fish Trematomus bernacchii was exposed to trace metals (Cd, Cu, Hg, Ni, Pb) and benzo[a]pyrene (BaP, as a model PAH), dosed alone and in combinations. Co-exposures revealed that BaP did not influence the accumulation of metals, while these elements caused significant changes on tissue levels of the PAH. The marked EROD induction caused by BaP was completely suppressed by co-exposure with Cd and Cu, but no effects were observed with Ni, Hg and Pb. Similar results were confirmed at the protein level by Western blot analyses while CYP1A1 mRNA levels were reduced only during Cd co-exposures. Clear evidence of oxidative perturbations was observed in fish co-treated with Cd and BaP and the reduced capability to absorb peroxyl and hydroxyl radicals suggested some oxidative pathways by which this element might indirectly modulate the biotransformation efficiency of Cytochrome P450. Partly different and post-transcriptional mechanisms of action could be hypothesized for Cu, while moderate oxidative effects of Hg, Ni and Pb during co-exposures would confirm their limited influence on metabolism of PAHs. In general, the overall results revealed a complex pathway of interactions between different chemicals during co-exposures and the importance of oxidative status in modulating induction and expression of CYP1A1. Article in Journal/Newspaper Antarc* Antarctic Antarctica Università Politecnica delle Marche: IRIS Antarctic The Antarctic Aquatic Toxicology 85 3 167 175 |
institution |
Open Polar |
collection |
Università Politecnica delle Marche: IRIS |
op_collection_id |
ftupmarcheiris |
language |
English |
topic |
Antarctica Bioaccumulation Cytochrome P450 Interactions Oxidative stress Polycyclic aromatic hydrocarbons Trace metals Trematomus bernacchii |
spellingShingle |
Antarctica Bioaccumulation Cytochrome P450 Interactions Oxidative stress Polycyclic aromatic hydrocarbons Trace metals Trematomus bernacchii BENEDETTI, MAURA MARTUCCIO G FATTORINI, DANIELE CANAPA, Adriana BARUCCA, MARCO NIGRO M REGOLI, Francesco Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii |
topic_facet |
Antarctica Bioaccumulation Cytochrome P450 Interactions Oxidative stress Polycyclic aromatic hydrocarbons Trace metals Trematomus bernacchii |
description |
Biological interactions between various classes of pollutants are of great relevance for the Antarctic marine environment, where the naturally elevated bioavailability of metals like cadmium might indirectly influence sensitivity of endemic organisms toward other environmental pollutants, e.g. polycyclic aromatic hydrocarbons (PAHs). To further investigate reciprocal effects of different chemicals, the fish Trematomus bernacchii was exposed to trace metals (Cd, Cu, Hg, Ni, Pb) and benzo[a]pyrene (BaP, as a model PAH), dosed alone and in combinations. Co-exposures revealed that BaP did not influence the accumulation of metals, while these elements caused significant changes on tissue levels of the PAH. The marked EROD induction caused by BaP was completely suppressed by co-exposure with Cd and Cu, but no effects were observed with Ni, Hg and Pb. Similar results were confirmed at the protein level by Western blot analyses while CYP1A1 mRNA levels were reduced only during Cd co-exposures. Clear evidence of oxidative perturbations was observed in fish co-treated with Cd and BaP and the reduced capability to absorb peroxyl and hydroxyl radicals suggested some oxidative pathways by which this element might indirectly modulate the biotransformation efficiency of Cytochrome P450. Partly different and post-transcriptional mechanisms of action could be hypothesized for Cu, while moderate oxidative effects of Hg, Ni and Pb during co-exposures would confirm their limited influence on metabolism of PAHs. In general, the overall results revealed a complex pathway of interactions between different chemicals during co-exposures and the importance of oxidative status in modulating induction and expression of CYP1A1. |
author2 |
Benedetti, Maura Martuccio, G Fattorini, Daniele Canapa, Adriana Barucca, Marco Nigro, M Regoli, Francesco |
format |
Article in Journal/Newspaper |
author |
BENEDETTI, MAURA MARTUCCIO G FATTORINI, DANIELE CANAPA, Adriana BARUCCA, MARCO NIGRO M REGOLI, Francesco |
author_facet |
BENEDETTI, MAURA MARTUCCIO G FATTORINI, DANIELE CANAPA, Adriana BARUCCA, MARCO NIGRO M REGOLI, Francesco |
author_sort |
BENEDETTI, MAURA |
title |
Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii |
title_short |
Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii |
title_full |
Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii |
title_fullStr |
Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii |
title_full_unstemmed |
Oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish Trematomus bernacchii |
title_sort |
oxidative and modulatory effects of trace metal on metabolism of polycyclic aromatic hydrocarbons in the antarctic fish trematomus bernacchii |
publishDate |
2007 |
url |
http://hdl.handle.net/11566/53440 https://doi.org/10.1016/j.aquatox.2007.08.009 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/17923160 info:eu-repo/semantics/altIdentifier/wos/WOS:000251639100001 volume:85 issue:3 firstpage:167 lastpage:175 numberofpages:9 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11566/53440 doi:10.1016/j.aquatox.2007.08.009 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-35448996882 |
op_doi |
https://doi.org/10.1016/j.aquatox.2007.08.009 |
container_title |
Aquatic Toxicology |
container_volume |
85 |
container_issue |
3 |
container_start_page |
167 |
op_container_end_page |
175 |
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1796300131216130048 |