Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii
Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified f...
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Online Access: | https://hdl.handle.net/11566/309185 https://doi.org/10.3390/microorganisms10122415 |
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ftupmarcheiris:oai:iris.univpm.it:11566/309185 2024-09-15T17:46:21+00:00 Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina 2022 https://hdl.handle.net/11566/309185 https://doi.org/10.3390/microorganisms10122415 eng eng info:eu-repo/semantics/altIdentifier/pmid/36557668 info:eu-repo/semantics/altIdentifier/wos/WOS:000903723800001 volume:10 issue:12 numberofpages:13 journal:MICROORGANISMS https://hdl.handle.net/11566/309185 doi:10.3390/microorganisms10122415 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85144668925 info:eu-repo/semantics/openAccess Tubulin function microtubule diversitie gene-silencing protozoa info:eu-repo/semantics/article 2022 ftupmarcheiris https://doi.org/10.3390/microorganisms10122415 2024-07-29T14:01:21Z Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified five β-tubulin isotypes by genome sequencing, named EFBTU1, EFBTU2, EFBTU3, EFBTU4 and EFBTU5. By using polyclonal antibodies directed against EFBTU2/EFBTU1 and EFBTU3, we show that the former isotypes appear to be involved in the formation of all microtubular structures and are particularly abundant in cilia, whereas the latter specifically localizes at the bases of cilia. By RNA interference (RNAi) technology, we silenced the EFBTU3 gene and provided evidence that this isotype has a relevant role in cilia regeneration upon deciliation and in cell division. These results support the long-standing concept that tubulin isotypes possess functional specificity in building diverse microtubular structures. Article in Journal/Newspaper Antarc* Antarctic Università Politecnica delle Marche: IRIS Microorganisms 10 12 2415 |
institution |
Open Polar |
collection |
Università Politecnica delle Marche: IRIS |
op_collection_id |
ftupmarcheiris |
language |
English |
topic |
Tubulin function microtubule diversitie gene-silencing protozoa |
spellingShingle |
Tubulin function microtubule diversitie gene-silencing protozoa Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
topic_facet |
Tubulin function microtubule diversitie gene-silencing protozoa |
description |
Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified five β-tubulin isotypes by genome sequencing, named EFBTU1, EFBTU2, EFBTU3, EFBTU4 and EFBTU5. By using polyclonal antibodies directed against EFBTU2/EFBTU1 and EFBTU3, we show that the former isotypes appear to be involved in the formation of all microtubular structures and are particularly abundant in cilia, whereas the latter specifically localizes at the bases of cilia. By RNA interference (RNAi) technology, we silenced the EFBTU3 gene and provided evidence that this isotype has a relevant role in cilia regeneration upon deciliation and in cell division. These results support the long-standing concept that tubulin isotypes possess functional specificity in building diverse microtubular structures. |
author2 |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina |
format |
Article in Journal/Newspaper |
author |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina |
author_facet |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina |
author_sort |
Pucciarelli, Sandra |
title |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_short |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_full |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_fullStr |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_full_unstemmed |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_sort |
ciliate microtubule diversities: insights from the efbtu3 tubulin in the antarctic ciliate euplotes focardii |
publishDate |
2022 |
url |
https://hdl.handle.net/11566/309185 https://doi.org/10.3390/microorganisms10122415 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/36557668 info:eu-repo/semantics/altIdentifier/wos/WOS:000903723800001 volume:10 issue:12 numberofpages:13 journal:MICROORGANISMS https://hdl.handle.net/11566/309185 doi:10.3390/microorganisms10122415 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85144668925 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/microorganisms10122415 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2415 |
_version_ |
1810494415390638080 |