Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements
High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is o...
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Online Access: | http://hdl.handle.net/11566/297162 https://doi.org/10.3390/w13213030 |
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ftupmarcheiris:oai:iris.univpm.it:11566/297162 2024-04-14T08:03:05+00:00 Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements Rivaro P. Vivado D. Falco P. Ianni C. Rivaro, P. Vivado, D. Falco, P. Ianni, C. 2021 ELETTRONICO http://hdl.handle.net/11566/297162 https://doi.org/10.3390/w13213030 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000719201000001 volume:13 issue:21 firstpage:3030 numberofpages:14 journal:WATER http://hdl.handle.net/11566/297162 doi:10.3390/w13213030 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85118612757 HPLC Meta-cresol purple Ocean acidification PH Ross Sea info:eu-repo/semantics/article 2021 ftupmarcheiris https://doi.org/10.3390/w13213030 2024-03-21T18:31:22Z High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and there-fore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH3 CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface. Article in Journal/Newspaper Antarc* Antarctica Ocean acidification Ross Sea Università Politecnica delle Marche: IRIS Ross Sea Water 13 21 3030 |
institution |
Open Polar |
collection |
Università Politecnica delle Marche: IRIS |
op_collection_id |
ftupmarcheiris |
language |
English |
topic |
HPLC Meta-cresol purple Ocean acidification PH Ross Sea |
spellingShingle |
HPLC Meta-cresol purple Ocean acidification PH Ross Sea Rivaro P. Vivado D. Falco P. Ianni C. Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
topic_facet |
HPLC Meta-cresol purple Ocean acidification PH Ross Sea |
description |
High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and there-fore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH3 CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface. |
author2 |
Rivaro, P. Vivado, D. Falco, P. Ianni, C. |
format |
Article in Journal/Newspaper |
author |
Rivaro P. Vivado D. Falco P. Ianni C. |
author_facet |
Rivaro P. Vivado D. Falco P. Ianni C. |
author_sort |
Rivaro P. |
title |
Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
title_short |
Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
title_full |
Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
title_fullStr |
Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
title_full_unstemmed |
Hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
title_sort |
hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
publishDate |
2021 |
url |
http://hdl.handle.net/11566/297162 https://doi.org/10.3390/w13213030 |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ocean acidification Ross Sea |
genre_facet |
Antarc* Antarctica Ocean acidification Ross Sea |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000719201000001 volume:13 issue:21 firstpage:3030 numberofpages:14 journal:WATER http://hdl.handle.net/11566/297162 doi:10.3390/w13213030 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85118612757 |
op_doi |
https://doi.org/10.3390/w13213030 |
container_title |
Water |
container_volume |
13 |
container_issue |
21 |
container_start_page |
3030 |
_version_ |
1796298357641052160 |