Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish.
This study investigated some aspects of xenobiotic metabolism in the Nototheniidae Trematomus bernacchii, a key sentinel species for monitoring Antarctic ecosystems. After laboratory exposure to β-naphthoflavone (βNF), basal levels and time-course induction of CYP1A, CYP1B and CYP3A were measured as...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | http://hdl.handle.net/11566/27290 https://doi.org/10.1016/j.aquatox.2007.05.010 |
id |
ftupmarcheiris:oai:iris.univpm.it:11566/27290 |
---|---|
record_format |
openpolar |
spelling |
ftupmarcheiris:oai:iris.univpm.it:11566/27290 2024-04-14T08:03:26+00:00 Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. DI BELLO D VACCAIO E LONGO V NIGRO M GERVASI PG PRETTI C. REGOLI, Francesco BENEDETTI, MAURA DI BELLO, D Vaccaio, E Longo, V Regoli, Francesco Nigro, M Benedetti, Maura Gervasi, Pg Pretti, C. 2007 http://hdl.handle.net/11566/27290 https://doi.org/10.1016/j.aquatox.2007.05.010 eng eng info:eu-repo/semantics/altIdentifier/pmid/17643506 info:eu-repo/semantics/altIdentifier/wos/WOS:000249165800003 volume:84 issue:1 firstpage:19 lastpage:26 numberofpages:8 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11566/27290 doi:10.1016/j.aquatox.2007.05.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34447625702 β-Naphthoflavone Antarctic fish CYP1A1 CYP1B1 DT-diaphorase GST Trematomus bernacchii UDP-GT info:eu-repo/semantics/article 2007 ftupmarcheiris https://doi.org/10.1016/j.aquatox.2007.05.010 2024-03-21T18:18:27Z This study investigated some aspects of xenobiotic metabolism in the Nototheniidae Trematomus bernacchii, a key sentinel species for monitoring Antarctic ecosystems. After laboratory exposure to β-naphthoflavone (βNF), basal levels and time-course induction of CYP1A, CYP1B and CYP3A were measured as enzymatic activities, immunoreactive protein content and mRNA expression in liver, gills, intestine and heart. Additional analyses in the liver included enzymatic activities of testosterone hydroxylase, (ω)- and (ω - 1)-lauric acid hydroxylase and some phase II enzymes related to the AhR battery genes, DT-diaphorase, glutathione S-transferases and UDP-glucuronyl transferases. Responsiveness of hepatic CYP1A1 after exposure to βNF demonstrated an higher sensitivity of MEROD than EROD activity and long lasting expression of mRNA still induced after 20 days from the treatment. Testosterone metabolism, oxidation of lauric acid and activities of phase II enzymes were not affected by βNF indicating that their modulation is not mediated by Ah receptor. Induction of CYP1A was more limited in gills and absent in intestine and heart. The first nucleotide sequence for CYP1B1 in an Antarctic fish has been obtained, revealing a homology of 89% and 72% respectively to CYP1B1 of plaice and CYP1B2 of carp. Constitutive expression of CYP1B1 was restricted to gills where it was also induced by βNF. Obtained results represent an additional contribution to the ecotoxicological characterization of T. bernacchii and further support the use of biomarkers for early detection of chemical pollution in Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctica Università Politecnica delle Marche: IRIS Antarctic Aquatic Toxicology 84 1 19 26 |
institution |
Open Polar |
collection |
Università Politecnica delle Marche: IRIS |
op_collection_id |
ftupmarcheiris |
language |
English |
topic |
β-Naphthoflavone Antarctic fish CYP1A1 CYP1B1 DT-diaphorase GST Trematomus bernacchii UDP-GT |
spellingShingle |
β-Naphthoflavone Antarctic fish CYP1A1 CYP1B1 DT-diaphorase GST Trematomus bernacchii UDP-GT DI BELLO D VACCAIO E LONGO V NIGRO M GERVASI PG PRETTI C. REGOLI, Francesco BENEDETTI, MAURA Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. |
topic_facet |
β-Naphthoflavone Antarctic fish CYP1A1 CYP1B1 DT-diaphorase GST Trematomus bernacchii UDP-GT |
description |
This study investigated some aspects of xenobiotic metabolism in the Nototheniidae Trematomus bernacchii, a key sentinel species for monitoring Antarctic ecosystems. After laboratory exposure to β-naphthoflavone (βNF), basal levels and time-course induction of CYP1A, CYP1B and CYP3A were measured as enzymatic activities, immunoreactive protein content and mRNA expression in liver, gills, intestine and heart. Additional analyses in the liver included enzymatic activities of testosterone hydroxylase, (ω)- and (ω - 1)-lauric acid hydroxylase and some phase II enzymes related to the AhR battery genes, DT-diaphorase, glutathione S-transferases and UDP-glucuronyl transferases. Responsiveness of hepatic CYP1A1 after exposure to βNF demonstrated an higher sensitivity of MEROD than EROD activity and long lasting expression of mRNA still induced after 20 days from the treatment. Testosterone metabolism, oxidation of lauric acid and activities of phase II enzymes were not affected by βNF indicating that their modulation is not mediated by Ah receptor. Induction of CYP1A was more limited in gills and absent in intestine and heart. The first nucleotide sequence for CYP1B1 in an Antarctic fish has been obtained, revealing a homology of 89% and 72% respectively to CYP1B1 of plaice and CYP1B2 of carp. Constitutive expression of CYP1B1 was restricted to gills where it was also induced by βNF. Obtained results represent an additional contribution to the ecotoxicological characterization of T. bernacchii and further support the use of biomarkers for early detection of chemical pollution in Antarctica. |
author2 |
DI BELLO, D Vaccaio, E Longo, V Regoli, Francesco Nigro, M Benedetti, Maura Gervasi, Pg Pretti, C. |
format |
Article in Journal/Newspaper |
author |
DI BELLO D VACCAIO E LONGO V NIGRO M GERVASI PG PRETTI C. REGOLI, Francesco BENEDETTI, MAURA |
author_facet |
DI BELLO D VACCAIO E LONGO V NIGRO M GERVASI PG PRETTI C. REGOLI, Francesco BENEDETTI, MAURA |
author_sort |
DI BELLO D |
title |
Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. |
title_short |
Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. |
title_full |
Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. |
title_fullStr |
Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. |
title_full_unstemmed |
Presence and inducibility by β-Naphtoflavone of CYP 1A1, CYP 1B1, UDP-GT, GST and DT-Diaphorase enzymes in Trematomus bernacchii, an Antarctic fish. |
title_sort |
presence and inducibility by β-naphtoflavone of cyp 1a1, cyp 1b1, udp-gt, gst and dt-diaphorase enzymes in trematomus bernacchii, an antarctic fish. |
publishDate |
2007 |
url |
http://hdl.handle.net/11566/27290 https://doi.org/10.1016/j.aquatox.2007.05.010 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/17643506 info:eu-repo/semantics/altIdentifier/wos/WOS:000249165800003 volume:84 issue:1 firstpage:19 lastpage:26 numberofpages:8 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11566/27290 doi:10.1016/j.aquatox.2007.05.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34447625702 |
op_doi |
https://doi.org/10.1016/j.aquatox.2007.05.010 |
container_title |
Aquatic Toxicology |
container_volume |
84 |
container_issue |
1 |
container_start_page |
19 |
op_container_end_page |
26 |
_version_ |
1796299694644658176 |