Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology
This work presents data on the quantification of fatty acids (FAs, in terms of mass unit per tissue weight) in the muscle of Trematomus bernacchii, a key species in Antarctica, often used as bioindicator for contamination studies. Modifications in fatty acids content should be considered a useful bi...
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Online Access: | http://hdl.handle.net/11566/246199 https://doi.org/10.1016/j.chemosphere.2016.12.140 |
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ftupmarcheiris:oai:iris.univpm.it:11566/246199 2024-04-14T08:01:55+00:00 Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology TRUZZI, Cristina ILLUMINATI, Silvia ANNIBALDI, ANNA ANTONUCCI, MATTEO SCARPONI, Giuseppe Truzzi, Cristina Illuminati, Silvia Annibaldi, Anna Antonucci, Matteo Scarponi, Giuseppe 2017 STAMPA http://hdl.handle.net/11566/246199 https://doi.org/10.1016/j.chemosphere.2016.12.140 eng eng info:eu-repo/semantics/altIdentifier/pmid/28107709 info:eu-repo/semantics/altIdentifier/wos/WOS:000395213700013 volume:173 firstpage:116 lastpage:123 numberofpages:8 journal:CHEMOSPHERE http://hdl.handle.net/11566/246199 doi:10.1016/j.chemosphere.2016.12.140 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85009484144 www.elsevier.com/locate/chemosphere Fatty acids quantification Gas chromatography-mass spectrometry Lipid profile Microwave-assisted extraction Muscle Trematomus bernacchii Chemistry (all) Environmental Chemistry info:eu-repo/semantics/article 2017 ftupmarcheiris https://doi.org/10.1016/j.chemosphere.2016.12.140 2024-03-21T18:24:12Z This work presents data on the quantification of fatty acids (FAs, in terms of mass unit per tissue weight) in the muscle of Trematomus bernacchii, a key species in Antarctica, often used as bioindicator for contamination studies. Modifications in fatty acids content should be considered a useful biomarker to study how contaminants affect Antarctic biota. Until now, very few studies quantified fatty acids of muscle of T. bernacchii, and only as percentage of a single fatty acid on total lipids. To perform the quantification of fatty acids, we used an analytical method based on a fast microwave-assisted extraction of lipids from a lyophilized sample, a base-catalyzed trans-esterification of lipid extract to obtain Fatty Acids Methyl Esters (FAMEs), and a separation and identification of FAMEs by gas chromatography-mass spectrometry. With the optimized and validated method, a fast and accurate separation of Fatty Acids Methyl Esters was performed in 43 min. The linearity was checked up to about 320 mg mL1; limit of detection and limit of quantification are in the range 4e22 mgmL1 and 13e66 mgmL1, respectively. The optimized method showed a good accuracy and precision. Major fatty acids were 14:0, 16:0, 16:1n7, 18:1n9, 18:1n7, 20:1n9, 20:5n3 and 22:6n3. Quantified FAs compute for about 47 mg g1 tissue dry weight (dw), with 9.1 ± 0.1 mg g1 dw of saturated FAs, 25.5 ± 0.1 mg g1 dw of mono-unsaturated FAs, and 12.2 ± 0.1 mg g1 dw of poly-unsaturated FAs Article in Journal/Newspaper Antarc* Antarctic Antarctica Università Politecnica delle Marche: IRIS Antarctic Chemosphere 173 116 123 |
institution |
Open Polar |
collection |
Università Politecnica delle Marche: IRIS |
op_collection_id |
ftupmarcheiris |
language |
English |
topic |
Fatty acids quantification Gas chromatography-mass spectrometry Lipid profile Microwave-assisted extraction Muscle Trematomus bernacchii Chemistry (all) Environmental Chemistry |
spellingShingle |
Fatty acids quantification Gas chromatography-mass spectrometry Lipid profile Microwave-assisted extraction Muscle Trematomus bernacchii Chemistry (all) Environmental Chemistry TRUZZI, Cristina ILLUMINATI, Silvia ANNIBALDI, ANNA ANTONUCCI, MATTEO SCARPONI, Giuseppe Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology |
topic_facet |
Fatty acids quantification Gas chromatography-mass spectrometry Lipid profile Microwave-assisted extraction Muscle Trematomus bernacchii Chemistry (all) Environmental Chemistry |
description |
This work presents data on the quantification of fatty acids (FAs, in terms of mass unit per tissue weight) in the muscle of Trematomus bernacchii, a key species in Antarctica, often used as bioindicator for contamination studies. Modifications in fatty acids content should be considered a useful biomarker to study how contaminants affect Antarctic biota. Until now, very few studies quantified fatty acids of muscle of T. bernacchii, and only as percentage of a single fatty acid on total lipids. To perform the quantification of fatty acids, we used an analytical method based on a fast microwave-assisted extraction of lipids from a lyophilized sample, a base-catalyzed trans-esterification of lipid extract to obtain Fatty Acids Methyl Esters (FAMEs), and a separation and identification of FAMEs by gas chromatography-mass spectrometry. With the optimized and validated method, a fast and accurate separation of Fatty Acids Methyl Esters was performed in 43 min. The linearity was checked up to about 320 mg mL1; limit of detection and limit of quantification are in the range 4e22 mgmL1 and 13e66 mgmL1, respectively. The optimized method showed a good accuracy and precision. Major fatty acids were 14:0, 16:0, 16:1n7, 18:1n9, 18:1n7, 20:1n9, 20:5n3 and 22:6n3. Quantified FAs compute for about 47 mg g1 tissue dry weight (dw), with 9.1 ± 0.1 mg g1 dw of saturated FAs, 25.5 ± 0.1 mg g1 dw of mono-unsaturated FAs, and 12.2 ± 0.1 mg g1 dw of poly-unsaturated FAs |
author2 |
Truzzi, Cristina Illuminati, Silvia Annibaldi, Anna Antonucci, Matteo Scarponi, Giuseppe |
format |
Article in Journal/Newspaper |
author |
TRUZZI, Cristina ILLUMINATI, Silvia ANNIBALDI, ANNA ANTONUCCI, MATTEO SCARPONI, Giuseppe |
author_facet |
TRUZZI, Cristina ILLUMINATI, Silvia ANNIBALDI, ANNA ANTONUCCI, MATTEO SCARPONI, Giuseppe |
author_sort |
TRUZZI, Cristina |
title |
Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology |
title_short |
Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology |
title_full |
Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology |
title_fullStr |
Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology |
title_full_unstemmed |
Quantification of fatty acids in the muscle of Antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry: Optimization of the analytical methodology |
title_sort |
quantification of fatty acids in the muscle of antarctic fish trematomus bernacchii by gas chromatography-mass spectrometry: optimization of the analytical methodology |
publishDate |
2017 |
url |
http://hdl.handle.net/11566/246199 https://doi.org/10.1016/j.chemosphere.2016.12.140 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/28107709 info:eu-repo/semantics/altIdentifier/wos/WOS:000395213700013 volume:173 firstpage:116 lastpage:123 numberofpages:8 journal:CHEMOSPHERE http://hdl.handle.net/11566/246199 doi:10.1016/j.chemosphere.2016.12.140 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85009484144 www.elsevier.com/locate/chemosphere |
op_doi |
https://doi.org/10.1016/j.chemosphere.2016.12.140 |
container_title |
Chemosphere |
container_volume |
173 |
container_start_page |
116 |
op_container_end_page |
123 |
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1796311640935759872 |