Population dynamics, interactions and evolution of marine microbes using genomic approaches
A la portada: Institut de Ciències del Mar (English) There is a myriad of microorganisms on Earth contributing to global biogeochemical cycles. In the surface ocean, the smallest microbes (picoplankton) are responsible for an important fraction of the total atmospheric carbon and nitrogen fixation....
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Universitat Politècnica de Catalunya
2023
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ftupcatalunyair:oai:upcommons.upc.edu:2117/396974 2024-09-15T18:24:17+00:00 Population dynamics, interactions and evolution of marine microbes using genomic approaches Latorre Pérez, Francisco Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental Logares Haurie, Ramiro Ernesto Jaillon, Olivier Mösso Aranda, César 2023-02-03 221 p. application/pdf http://hdl.handle.net/2117/396974 http://hdl.handle.net/10803/689381 https://doi.org/10.5821/dissertation-2117-396974 eng eng Universitat Politècnica de Catalunya Latorre Pérez, F. Population dynamics, interactions and evolution of marine microbes using genomic approaches. Tesi doctoral, UPC, Departament d'Enginyeria Civil i Ambiental, 2023. DOI 10.5821/dissertation-2117-396974 . http://hdl.handle.net/2117/396974 doi:10.5821/dissertation-2117-396974 http://hdl.handle.net/10803/689381 L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/4.0/ http://creativecommons.org/licenses/by-nc-sa/4.0/ Open Access TDX (Tesis Doctorals en Xarxa) Population dynamics Evolution Interactions Marine microbes Protists High-throughput sequencing Single-cell Genomics Marine ecology Metagenomics Marine environment Doctoral thesis 2023 ftupcatalunyair https://doi.org/10.5821/dissertation-2117-396974 2024-07-25T11:08:02Z A la portada: Institut de Ciències del Mar (English) There is a myriad of microorganisms on Earth contributing to global biogeochemical cycles. In the surface ocean, the smallest microbes (picoplankton) are responsible for an important fraction of the total atmospheric carbon and nitrogen fixation. The ocean picoplankton encompasses both prokaryotes (bacteria and archaea) and tiny unicellular eukaryotes (protists). Despite their overall importance for the functioning of the biosphere, many questions remain unanswered on their biogeography, population dynamics, interactions, and evolution. Answering these questions is essential in the context of global change, as alterations of the ocean microbiome could impact the function of multiple ecosystems. In this thesis, we aim at reducing the knowledge gap on the above topics through the application of High-Throughput Sequencing (HTS) and genomic approaches, using data collected during the circumglobal Tara Oceans and Malaspina-2010 expeditions, as well as at the Gulf of Maine (North Atlantic), and two Northwestern Mediterranean coastal microbial observatories (BBMO and SOLA stations). Chapters 1 and 2 are dedicated to a small protistan group of heterotrophic flagellates (HF): the Marine Stramenopiles (MAST)-4, relevant during picoplankton grazing and nutrient remineralization. Due to its widespread distribution and relatively high abundance, MAST-4 has become a target group of microbes to study HF. Unfortunately, MAST-4 remains uncultured. We investigated four evolutionary-related species of MAST-4 (species A, B, C, and E) by reconstructing their genomes with Single-Cell genomics data. In chapter 1, co-occurrence and biogeographic analyses in the surface global ocean indicated contrasting patterns driven by temperature. Although MAST-4 species were similar in terms of broad metabolic functions, they differed in the set of genes related to the food degradation machinery. We proposed that differential niche adaptation to temperature and prey type has promoted the ... Doctoral or Postdoctoral Thesis North Atlantic Universitat Politècnica de Catalunya, BarcelonaTech: UPCommons - Global access to UPC knowledge |
institution |
Open Polar |
collection |
Universitat Politècnica de Catalunya, BarcelonaTech: UPCommons - Global access to UPC knowledge |
op_collection_id |
ftupcatalunyair |
language |
English |
topic |
Population dynamics Evolution Interactions Marine microbes Protists High-throughput sequencing Single-cell Genomics Marine ecology Metagenomics Marine environment |
spellingShingle |
Population dynamics Evolution Interactions Marine microbes Protists High-throughput sequencing Single-cell Genomics Marine ecology Metagenomics Marine environment Latorre Pérez, Francisco Population dynamics, interactions and evolution of marine microbes using genomic approaches |
topic_facet |
Population dynamics Evolution Interactions Marine microbes Protists High-throughput sequencing Single-cell Genomics Marine ecology Metagenomics Marine environment |
description |
A la portada: Institut de Ciències del Mar (English) There is a myriad of microorganisms on Earth contributing to global biogeochemical cycles. In the surface ocean, the smallest microbes (picoplankton) are responsible for an important fraction of the total atmospheric carbon and nitrogen fixation. The ocean picoplankton encompasses both prokaryotes (bacteria and archaea) and tiny unicellular eukaryotes (protists). Despite their overall importance for the functioning of the biosphere, many questions remain unanswered on their biogeography, population dynamics, interactions, and evolution. Answering these questions is essential in the context of global change, as alterations of the ocean microbiome could impact the function of multiple ecosystems. In this thesis, we aim at reducing the knowledge gap on the above topics through the application of High-Throughput Sequencing (HTS) and genomic approaches, using data collected during the circumglobal Tara Oceans and Malaspina-2010 expeditions, as well as at the Gulf of Maine (North Atlantic), and two Northwestern Mediterranean coastal microbial observatories (BBMO and SOLA stations). Chapters 1 and 2 are dedicated to a small protistan group of heterotrophic flagellates (HF): the Marine Stramenopiles (MAST)-4, relevant during picoplankton grazing and nutrient remineralization. Due to its widespread distribution and relatively high abundance, MAST-4 has become a target group of microbes to study HF. Unfortunately, MAST-4 remains uncultured. We investigated four evolutionary-related species of MAST-4 (species A, B, C, and E) by reconstructing their genomes with Single-Cell genomics data. In chapter 1, co-occurrence and biogeographic analyses in the surface global ocean indicated contrasting patterns driven by temperature. Although MAST-4 species were similar in terms of broad metabolic functions, they differed in the set of genes related to the food degradation machinery. We proposed that differential niche adaptation to temperature and prey type has promoted the ... |
author2 |
Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental Logares Haurie, Ramiro Ernesto Jaillon, Olivier Mösso Aranda, César |
format |
Doctoral or Postdoctoral Thesis |
author |
Latorre Pérez, Francisco |
author_facet |
Latorre Pérez, Francisco |
author_sort |
Latorre Pérez, Francisco |
title |
Population dynamics, interactions and evolution of marine microbes using genomic approaches |
title_short |
Population dynamics, interactions and evolution of marine microbes using genomic approaches |
title_full |
Population dynamics, interactions and evolution of marine microbes using genomic approaches |
title_fullStr |
Population dynamics, interactions and evolution of marine microbes using genomic approaches |
title_full_unstemmed |
Population dynamics, interactions and evolution of marine microbes using genomic approaches |
title_sort |
population dynamics, interactions and evolution of marine microbes using genomic approaches |
publisher |
Universitat Politècnica de Catalunya |
publishDate |
2023 |
url |
http://hdl.handle.net/2117/396974 http://hdl.handle.net/10803/689381 https://doi.org/10.5821/dissertation-2117-396974 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
TDX (Tesis Doctorals en Xarxa) |
op_relation |
Latorre Pérez, F. Population dynamics, interactions and evolution of marine microbes using genomic approaches. Tesi doctoral, UPC, Departament d'Enginyeria Civil i Ambiental, 2023. DOI 10.5821/dissertation-2117-396974 . http://hdl.handle.net/2117/396974 doi:10.5821/dissertation-2117-396974 http://hdl.handle.net/10803/689381 |
op_rights |
L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/4.0/ http://creativecommons.org/licenses/by-nc-sa/4.0/ Open Access |
op_doi |
https://doi.org/10.5821/dissertation-2117-396974 |
_version_ |
1810464619290951680 |