High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake
Deep Lake in Antarctica is a globally isolated, hypersaline system that remains liquid at temperatures down to -20 °C. By analyzing metagenome data and genomes of four isolates we assessed genome variation and patterns of gene exchange to learn how the lake community evolved. The lake is completely...
Published in: | Proceedings of the National Academy of Sciences |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Proceedings of the National Academy of Sciences
2013
|
Subjects: | |
Online Access: | http://hdl.handle.net/1959.4/unsworks_49885 https://unsworks.unsw.edu.au/bitstreams/632bdde0-ab2d-4ecc-9ad5-7fe57269ba92/download https://doi.org/10.1073/pnas.1307090110 |
id |
ftunswworks:oai:unsworks.library.unsw.edu.au:1959.4/unsworks_49885 |
---|---|
record_format |
openpolar |
spelling |
ftunswworks:oai:unsworks.library.unsw.edu.au:1959.4/unsworks_49885 2024-05-19T07:31:02+00:00 High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake Demaere, MZ Williams, TJ Allen, MA Brown, MV Gibson, JAE Rich, J Lauro, FM Dyall-Smith, M Davenport, KW Woyke, T Kyrpides, NC Tringe, SG Cavicchioli, R 2013-10-15 application/pdf http://hdl.handle.net/1959.4/unsworks_49885 https://unsworks.unsw.edu.au/bitstreams/632bdde0-ab2d-4ecc-9ad5-7fe57269ba92/download https://doi.org/10.1073/pnas.1307090110 unknown Proceedings of the National Academy of Sciences http://purl.org/au-research/grants/arc/DP110103232 http://hdl.handle.net/1959.4/unsworks_49885 https://unsworks.unsw.edu.au/bitstreams/632bdde0-ab2d-4ecc-9ad5-7fe57269ba92/download https://doi.org/10.1073/pnas.1307090110 open access https://purl.org/coar/access_right/c_abf2 CC-BY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/ free_to_read urn:ISSN:0027-8424 urn:ISSN:1091-6490 Proceedings of the National Academy of Sciences of the United States of America, 110, 42, 16939-16944 Genetics Human Genome Biotechnology Antarctic Regions Evolution Molecular Gene Transfer Horizontal Genome Archaeal Halobacteriaceae Lakes Metagenome RNA Ribosomal 16S Water Microbiology Antarctic haloarchaea BJ1 virus fragment recruitment mobile genetic elements saltern journal article http://purl.org/coar/resource_type/c_6501 2013 ftunswworks https://doi.org/10.1073/pnas.1307090110 2024-04-24T00:19:56Z Deep Lake in Antarctica is a globally isolated, hypersaline system that remains liquid at temperatures down to -20 °C. By analyzing metagenome data and genomes of four isolates we assessed genome variation and patterns of gene exchange to learn how the lake community evolved. The lake is completely and uniformly dominated by haloarchaea, comprising a hierarchically structured, low-complexity community that differs greatly to temperate and tropical hypersaline environments. The four Deep Lake isolates represent distinct genera (~85% 16S rRNA gene similarity and ~73% genome average nucleotide identity) with genomic characteristics indicative of niche adaptation, and collectively account for ~72% of the cellular community. Network analysis revealed a remarkable level of intergenera gene exchange, including the sharing of long contiguous regions (up to 35 kb) of high identity (~100%). Although the genomes of closely related Halobacterium, Haloquadratum, and Haloarcula (>90% average nucleotide identity) shared regions of high identity between species or strains, the four Deep Lake isolates were the only distantly related haloarchaea to share long high-identity regions. Moreover, the Deep Lake high-identity regions did not match to any other hypersaline environment metagenome data. The most abundant species, tADL, appears to play a central role in the exchange of insertion sequences, but not the exchange of high-identity regions. The genomic characteristics of the four haloarchaea are consistent with a lake ecosystem that sustains a high level of intergenera gene exchange while selecting for ecotypes that maintain sympatric speciation. The peculiarities of this polar system restrict which species can grow and provide a tempo and mode for accentuating gene exchange. Article in Journal/Newspaper Antarc* Antarctic Antarctica UNSW Sydney (The University of New South Wales): UNSWorks Proceedings of the National Academy of Sciences 110 42 16939 16944 |
institution |
Open Polar |
collection |
UNSW Sydney (The University of New South Wales): UNSWorks |
op_collection_id |
ftunswworks |
language |
unknown |
topic |
Genetics Human Genome Biotechnology Antarctic Regions Evolution Molecular Gene Transfer Horizontal Genome Archaeal Halobacteriaceae Lakes Metagenome RNA Ribosomal 16S Water Microbiology Antarctic haloarchaea BJ1 virus fragment recruitment mobile genetic elements saltern |
spellingShingle |
Genetics Human Genome Biotechnology Antarctic Regions Evolution Molecular Gene Transfer Horizontal Genome Archaeal Halobacteriaceae Lakes Metagenome RNA Ribosomal 16S Water Microbiology Antarctic haloarchaea BJ1 virus fragment recruitment mobile genetic elements saltern Demaere, MZ Williams, TJ Allen, MA Brown, MV Gibson, JAE Rich, J Lauro, FM Dyall-Smith, M Davenport, KW Woyke, T Kyrpides, NC Tringe, SG Cavicchioli, R High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake |
topic_facet |
Genetics Human Genome Biotechnology Antarctic Regions Evolution Molecular Gene Transfer Horizontal Genome Archaeal Halobacteriaceae Lakes Metagenome RNA Ribosomal 16S Water Microbiology Antarctic haloarchaea BJ1 virus fragment recruitment mobile genetic elements saltern |
description |
Deep Lake in Antarctica is a globally isolated, hypersaline system that remains liquid at temperatures down to -20 °C. By analyzing metagenome data and genomes of four isolates we assessed genome variation and patterns of gene exchange to learn how the lake community evolved. The lake is completely and uniformly dominated by haloarchaea, comprising a hierarchically structured, low-complexity community that differs greatly to temperate and tropical hypersaline environments. The four Deep Lake isolates represent distinct genera (~85% 16S rRNA gene similarity and ~73% genome average nucleotide identity) with genomic characteristics indicative of niche adaptation, and collectively account for ~72% of the cellular community. Network analysis revealed a remarkable level of intergenera gene exchange, including the sharing of long contiguous regions (up to 35 kb) of high identity (~100%). Although the genomes of closely related Halobacterium, Haloquadratum, and Haloarcula (>90% average nucleotide identity) shared regions of high identity between species or strains, the four Deep Lake isolates were the only distantly related haloarchaea to share long high-identity regions. Moreover, the Deep Lake high-identity regions did not match to any other hypersaline environment metagenome data. The most abundant species, tADL, appears to play a central role in the exchange of insertion sequences, but not the exchange of high-identity regions. The genomic characteristics of the four haloarchaea are consistent with a lake ecosystem that sustains a high level of intergenera gene exchange while selecting for ecotypes that maintain sympatric speciation. The peculiarities of this polar system restrict which species can grow and provide a tempo and mode for accentuating gene exchange. |
format |
Article in Journal/Newspaper |
author |
Demaere, MZ Williams, TJ Allen, MA Brown, MV Gibson, JAE Rich, J Lauro, FM Dyall-Smith, M Davenport, KW Woyke, T Kyrpides, NC Tringe, SG Cavicchioli, R |
author_facet |
Demaere, MZ Williams, TJ Allen, MA Brown, MV Gibson, JAE Rich, J Lauro, FM Dyall-Smith, M Davenport, KW Woyke, T Kyrpides, NC Tringe, SG Cavicchioli, R |
author_sort |
Demaere, MZ |
title |
High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake |
title_short |
High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake |
title_full |
High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake |
title_fullStr |
High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake |
title_full_unstemmed |
High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake |
title_sort |
high level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated antarctic lake |
publisher |
Proceedings of the National Academy of Sciences |
publishDate |
2013 |
url |
http://hdl.handle.net/1959.4/unsworks_49885 https://unsworks.unsw.edu.au/bitstreams/632bdde0-ab2d-4ecc-9ad5-7fe57269ba92/download https://doi.org/10.1073/pnas.1307090110 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
urn:ISSN:0027-8424 urn:ISSN:1091-6490 Proceedings of the National Academy of Sciences of the United States of America, 110, 42, 16939-16944 |
op_relation |
http://purl.org/au-research/grants/arc/DP110103232 http://hdl.handle.net/1959.4/unsworks_49885 https://unsworks.unsw.edu.au/bitstreams/632bdde0-ab2d-4ecc-9ad5-7fe57269ba92/download https://doi.org/10.1073/pnas.1307090110 |
op_rights |
open access https://purl.org/coar/access_right/c_abf2 CC-BY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/ free_to_read |
op_doi |
https://doi.org/10.1073/pnas.1307090110 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
110 |
container_issue |
42 |
container_start_page |
16939 |
op_container_end_page |
16944 |
_version_ |
1799468878245396480 |