Genetics and proteomics of haloarchaea from Deep Lake, Antarctica
Psychrophilic haloarchaea dominate life in Deep Lake, a monomictic hypersaline lake in the Vestfold Hills of Antarctica. Halorubrum lacusprofundi and Halohasta litchfieldiae represent ~ 10% and ~ 44% of the Deep Lake community, respectively. Their relative abundance has been linked to genomic distin...
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UNSW, Sydney
2017
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ftunswworks:oai:unsworks.library.unsw.edu.au:1959.4/57339 2023-05-15T13:48:45+02:00 Genetics and proteomics of haloarchaea from Deep Lake, Antarctica Liao, Yan 2017 application/pdf http://hdl.handle.net/1959.4/57339 https://unsworks.unsw.edu.au/bitstreams/484cde49-fe88-4e39-82d9-8ae3a3649413/download https://doi.org/10.26190/unsworks/3129 EN eng UNSW, Sydney http://hdl.handle.net/1959.4/57339 https://unsworks.unsw.edu.au/bitstreams/484cde49-fe88-4e39-82d9-8ae3a3649413/download https://doi.org/10.26190/unsworks/3129 open access https://purl.org/coar/access_right/c_abf2 CC BY-NC-ND 3.0 https://creativecommons.org/licenses/by-nc-nd/3.0/au/ free_to_read CC-BY-NC-ND Proteomics Psychrophilic haloarchaea Genetics Cold adaptation Antarctica doctoral thesis http://purl.org/coar/resource_type/c_db06 2017 ftunswworks https://doi.org/10.26190/unsworks/3129 2022-08-09T07:32:57Z Psychrophilic haloarchaea dominate life in Deep Lake, a monomictic hypersaline lake in the Vestfold Hills of Antarctica. Halorubrum lacusprofundi and Halohasta litchfieldiae represent ~ 10% and ~ 44% of the Deep Lake community, respectively. Their relative abundance has been linked to genomic distinctions including an ability to utilize specific nutrients. A gene transfer system was developed for Hrr. lacusprofundi, the first for any psychrophilic Archaea. A gene knockout was constructed for an acetamidase/formamidase gene, which represents a class of genes not previously experimentally studied in Archaea. Disruption of the gene resulted in the inability of cells to utilize acetamide for growth. By investigating the phylogeny of acetamidases/formamidases in Bacteria and Archaea and the environmental relevance of acetamide, it was concluded that accumulation of acetamide-related pollutants may promote the selection of species harboring acetamidase/formamidase genes. An integrative approach combining microscopy and quantitative proteomics enabled a comprehensive investigation of ecologically relevant mechanisms of growth, survival and speciation of the two species. Specific growth temperatures, substrates and growth phase led to biofilm formation in Hrr. lacusprofundi, with extracellular DNA and quorum sensing linked to biofilm formation. Sucrose metabolism in Hht. litchfieldiae was shown to increase carbohydrate uptake and glycolysis and decrease pyruvate uptake and metabolism, TCA cycle, glycerol metabolism and gluconeogenesis. Both species responded to low temperature by modifying their cell morphology and cell envelope, maintaining osmotic balance and translation initiation, and altering RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies and pathways for metabolism of glycerol and pyruvate. Low temperature enhanced the levels of CRISPR Cas proteins and the core gene expression machinery in Hht. litchfieldiae. A novel pathway for PHA granule ... Doctoral or Postdoctoral Thesis Antarc* Antarctica UNSW Sydney (The University of New South Wales): UNSWorks Vestfold Vestfold Hills |
institution |
Open Polar |
collection |
UNSW Sydney (The University of New South Wales): UNSWorks |
op_collection_id |
ftunswworks |
language |
English |
topic |
Proteomics Psychrophilic haloarchaea Genetics Cold adaptation Antarctica |
spellingShingle |
Proteomics Psychrophilic haloarchaea Genetics Cold adaptation Antarctica Liao, Yan Genetics and proteomics of haloarchaea from Deep Lake, Antarctica |
topic_facet |
Proteomics Psychrophilic haloarchaea Genetics Cold adaptation Antarctica |
description |
Psychrophilic haloarchaea dominate life in Deep Lake, a monomictic hypersaline lake in the Vestfold Hills of Antarctica. Halorubrum lacusprofundi and Halohasta litchfieldiae represent ~ 10% and ~ 44% of the Deep Lake community, respectively. Their relative abundance has been linked to genomic distinctions including an ability to utilize specific nutrients. A gene transfer system was developed for Hrr. lacusprofundi, the first for any psychrophilic Archaea. A gene knockout was constructed for an acetamidase/formamidase gene, which represents a class of genes not previously experimentally studied in Archaea. Disruption of the gene resulted in the inability of cells to utilize acetamide for growth. By investigating the phylogeny of acetamidases/formamidases in Bacteria and Archaea and the environmental relevance of acetamide, it was concluded that accumulation of acetamide-related pollutants may promote the selection of species harboring acetamidase/formamidase genes. An integrative approach combining microscopy and quantitative proteomics enabled a comprehensive investigation of ecologically relevant mechanisms of growth, survival and speciation of the two species. Specific growth temperatures, substrates and growth phase led to biofilm formation in Hrr. lacusprofundi, with extracellular DNA and quorum sensing linked to biofilm formation. Sucrose metabolism in Hht. litchfieldiae was shown to increase carbohydrate uptake and glycolysis and decrease pyruvate uptake and metabolism, TCA cycle, glycerol metabolism and gluconeogenesis. Both species responded to low temperature by modifying their cell morphology and cell envelope, maintaining osmotic balance and translation initiation, and altering RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies and pathways for metabolism of glycerol and pyruvate. Low temperature enhanced the levels of CRISPR Cas proteins and the core gene expression machinery in Hht. litchfieldiae. A novel pathway for PHA granule ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Liao, Yan |
author_facet |
Liao, Yan |
author_sort |
Liao, Yan |
title |
Genetics and proteomics of haloarchaea from Deep Lake, Antarctica |
title_short |
Genetics and proteomics of haloarchaea from Deep Lake, Antarctica |
title_full |
Genetics and proteomics of haloarchaea from Deep Lake, Antarctica |
title_fullStr |
Genetics and proteomics of haloarchaea from Deep Lake, Antarctica |
title_full_unstemmed |
Genetics and proteomics of haloarchaea from Deep Lake, Antarctica |
title_sort |
genetics and proteomics of haloarchaea from deep lake, antarctica |
publisher |
UNSW, Sydney |
publishDate |
2017 |
url |
http://hdl.handle.net/1959.4/57339 https://unsworks.unsw.edu.au/bitstreams/484cde49-fe88-4e39-82d9-8ae3a3649413/download https://doi.org/10.26190/unsworks/3129 |
geographic |
Vestfold Vestfold Hills |
geographic_facet |
Vestfold Vestfold Hills |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://hdl.handle.net/1959.4/57339 https://unsworks.unsw.edu.au/bitstreams/484cde49-fe88-4e39-82d9-8ae3a3649413/download https://doi.org/10.26190/unsworks/3129 |
op_rights |
open access https://purl.org/coar/access_right/c_abf2 CC BY-NC-ND 3.0 https://creativecommons.org/licenses/by-nc-nd/3.0/au/ free_to_read |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.26190/unsworks/3129 |
_version_ |
1766249659990278144 |