Nitrogen fixing potential in extreme environments
Biological nitrogen fixation is a key process in providing accessible nitrogen to Earth s biosphere. This process has been studied in various habitats yet extreme environments still remain relatively unexplored. The nifH gene codes for the Fe protein component in the nitrogenase, which facilitates t...
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UNSW, Sydney
2013
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ftunswworks:oai:unsworks.library.unsw.edu.au:1959.4/52826 2023-05-15T13:52:09+02:00 Nitrogen fixing potential in extreme environments Sorek Abramovich, Reut 2013 application/pdf http://hdl.handle.net/1959.4/52826 https://unsworks.unsw.edu.au/bitstreams/94aee845-8596-411c-a3f5-acd9290f500e/download https://doi.org/10.26190/unsworks/16288 EN eng UNSW, Sydney http://hdl.handle.net/1959.4/52826 https://unsworks.unsw.edu.au/bitstreams/94aee845-8596-411c-a3f5-acd9290f500e/download https://doi.org/10.26190/unsworks/16288 open access https://purl.org/coar/access_right/c_abf2 CC BY-NC-ND 3.0 https://creativecommons.org/licenses/by-nc-nd/3.0/au/ free_to_read CC-BY-NC-ND Nitrogen Stromatolites Extremophile nifH doctoral thesis http://purl.org/coar/resource_type/c_db06 2013 ftunswworks https://doi.org/10.26190/unsworks/16288 2022-08-09T07:41:23Z Biological nitrogen fixation is a key process in providing accessible nitrogen to Earth s biosphere. This process has been studied in various habitats yet extreme environments still remain relatively unexplored. The nifH gene codes for the Fe protein component in the nitrogenase, which facilitates the nitrogen fixation. Our aims in this study were to assess diazotrophic diversity, richness and community structure in three unique environments and analyse potential adaptations in the Fe protein composition and structure. Our methods included a terminal-restriction fragment length polymorphism (T-RFLP) analysis on 16S rDNA, PCR amplification of the nifH gene, statistical t-test analysis of amino acid compositions, a novel evolutionary analysis and 3D modelling with the I-TASSER web server. Boulder Clay and Amorphous Glacier are two ice-free areas in Terra Nova Bay, Antarctica, which differ in their geological origins and physio-chemical properties. DNA yields from ice-core samples ranged from 0.29 ng L-1 in Amorphous Glacier to 88 ng L-1 in Boulder Clay. Bray-Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from Amorphous Glacier. The hypersaline (>70 ppt) bays of Shark Bay, Western Australia, are home to the stromatolites microbial mats. The microbial diversity of diazotrophs from two different years, 1996 and 2004, was investigated. Our analysis indicated columnar stromatolites included a common persisting cyanobacterial diazotroph, a Cyanothece or Xenoccocous. Both samples contained novel nifH gene sequences of low similarity to uncultured nifH clones from saline to hypersaline environments, and their inferred NifH amino acid sequences were highly similar to unicellular, non-heterocystous Cyanobacteria and γ,-Proteobacteria sequences. Paralana s hot radon springs (PHS, 57 C°) are situated in South Australia. Phylogenetic analysis indicated a rich and diverse group of amino acid NifH sequences from α-, γ-, and δ-Proteobacteria, Chloroflexi ... Doctoral or Postdoctoral Thesis Antarc* Antarctica ice core UNSW Sydney (The University of New South Wales): UNSWorks Amorphous Glacier ENVELOPE(163.933,163.933,-74.700,-74.700) Bray ENVELOPE(-114.067,-114.067,-74.833,-74.833) Terra Nova Bay |
institution |
Open Polar |
collection |
UNSW Sydney (The University of New South Wales): UNSWorks |
op_collection_id |
ftunswworks |
language |
English |
topic |
Nitrogen Stromatolites Extremophile nifH |
spellingShingle |
Nitrogen Stromatolites Extremophile nifH Sorek Abramovich, Reut Nitrogen fixing potential in extreme environments |
topic_facet |
Nitrogen Stromatolites Extremophile nifH |
description |
Biological nitrogen fixation is a key process in providing accessible nitrogen to Earth s biosphere. This process has been studied in various habitats yet extreme environments still remain relatively unexplored. The nifH gene codes for the Fe protein component in the nitrogenase, which facilitates the nitrogen fixation. Our aims in this study were to assess diazotrophic diversity, richness and community structure in three unique environments and analyse potential adaptations in the Fe protein composition and structure. Our methods included a terminal-restriction fragment length polymorphism (T-RFLP) analysis on 16S rDNA, PCR amplification of the nifH gene, statistical t-test analysis of amino acid compositions, a novel evolutionary analysis and 3D modelling with the I-TASSER web server. Boulder Clay and Amorphous Glacier are two ice-free areas in Terra Nova Bay, Antarctica, which differ in their geological origins and physio-chemical properties. DNA yields from ice-core samples ranged from 0.29 ng L-1 in Amorphous Glacier to 88 ng L-1 in Boulder Clay. Bray-Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from Amorphous Glacier. The hypersaline (>70 ppt) bays of Shark Bay, Western Australia, are home to the stromatolites microbial mats. The microbial diversity of diazotrophs from two different years, 1996 and 2004, was investigated. Our analysis indicated columnar stromatolites included a common persisting cyanobacterial diazotroph, a Cyanothece or Xenoccocous. Both samples contained novel nifH gene sequences of low similarity to uncultured nifH clones from saline to hypersaline environments, and their inferred NifH amino acid sequences were highly similar to unicellular, non-heterocystous Cyanobacteria and γ,-Proteobacteria sequences. Paralana s hot radon springs (PHS, 57 C°) are situated in South Australia. Phylogenetic analysis indicated a rich and diverse group of amino acid NifH sequences from α-, γ-, and δ-Proteobacteria, Chloroflexi ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Sorek Abramovich, Reut |
author_facet |
Sorek Abramovich, Reut |
author_sort |
Sorek Abramovich, Reut |
title |
Nitrogen fixing potential in extreme environments |
title_short |
Nitrogen fixing potential in extreme environments |
title_full |
Nitrogen fixing potential in extreme environments |
title_fullStr |
Nitrogen fixing potential in extreme environments |
title_full_unstemmed |
Nitrogen fixing potential in extreme environments |
title_sort |
nitrogen fixing potential in extreme environments |
publisher |
UNSW, Sydney |
publishDate |
2013 |
url |
http://hdl.handle.net/1959.4/52826 https://unsworks.unsw.edu.au/bitstreams/94aee845-8596-411c-a3f5-acd9290f500e/download https://doi.org/10.26190/unsworks/16288 |
long_lat |
ENVELOPE(163.933,163.933,-74.700,-74.700) ENVELOPE(-114.067,-114.067,-74.833,-74.833) |
geographic |
Amorphous Glacier Bray Terra Nova Bay |
geographic_facet |
Amorphous Glacier Bray Terra Nova Bay |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_relation |
http://hdl.handle.net/1959.4/52826 https://unsworks.unsw.edu.au/bitstreams/94aee845-8596-411c-a3f5-acd9290f500e/download https://doi.org/10.26190/unsworks/16288 |
op_rights |
open access https://purl.org/coar/access_right/c_abf2 CC BY-NC-ND 3.0 https://creativecommons.org/licenses/by-nc-nd/3.0/au/ free_to_read |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.26190/unsworks/16288 |
_version_ |
1766256418018557952 |