Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing
Although general models of the processes involved in insect survival of freezing exist, there have been few studies directly investigating physiological processes during cooling, freezing and thawing, without which these models remain hypothetical. Here, we use open-flow respirometry to investigate...
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ftunstellenbosch:oai:scholar.sun.ac.za:10019.1/12952 2023-11-12T04:08:10+01:00 Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing Sinclair B.J. Klok C.J. Chown S.L. 2004 http://hdl.handle.net/10019.1/12952 https://doi.org/10.1242/jeb.00880 unknown Journal of Experimental Biology 207 8 220949 doi:10.1242/jeb.00880 http://hdl.handle.net/10019.1/12952 carbon dioxide animal article body temperature comparative study energy metabolism freezing geography metabolism moth oxygen consumption physiology South Africa Animals Moths Animalia Insecta Invertebrata Lepidoptera Phacelia congesta Tineidae Article 2004 ftunstellenbosch https://doi.org/10.1242/jeb.00880 2023-10-22T07:38:05Z Although general models of the processes involved in insect survival of freezing exist, there have been few studies directly investigating physiological processes during cooling, freezing and thawing, without which these models remain hypothetical. Here, we use open-flow respirometry to investigate the metabolism of the freeze-tolerant sub-Antarctic caterpillar Pringleophaga marioni Viette (Lepidoptera: Tineidae) during cooling, freezing and thawing and to compare animals exposed to non-lethal (-5.8°C) and lethal (-6.0°C, after which caterpillars are moribund for several days, and -18°C, after which caterpillars are completely unresponsive) freezing stress. We found a large decrease in metabolic rate (that is not associated with freezing) at -0.6±0.1°C and calculated a Q10 of 2.14×10 3 at this breakpoint. This breakpoint is coincident with the critical thermal minimum (CTmin) and is hypothesised to be a metabolic manifestation of the latter, possibly a failure of the Na+/K +-ATPase pump. This provides a plausible link between processes at the cellular level and observations of the action of the CTmin at tissue and whole-organism levels. Caterpillars froze at -4.6±0.1°C and had detectable metabolism when frozen. Post-thaw, metabolic rates were lower than pre-freering measurements. Post-thaw metabolic rates did not differ between temperatures that did and did not kill the caterpillars, which suggests that mortality may be a result of a breakdown in processes at the organismal, rather than cellular, level of organisation. Article Article in Journal/Newspaper Antarc* Antarctic Stellenbosch University: SUNScholar Research Repository Antarctic Journal of Experimental Biology 207 8 1287 1294 |
institution |
Open Polar |
collection |
Stellenbosch University: SUNScholar Research Repository |
op_collection_id |
ftunstellenbosch |
language |
unknown |
topic |
carbon dioxide animal article body temperature comparative study energy metabolism freezing geography metabolism moth oxygen consumption physiology South Africa Animals Moths Animalia Insecta Invertebrata Lepidoptera Phacelia congesta Tineidae |
spellingShingle |
carbon dioxide animal article body temperature comparative study energy metabolism freezing geography metabolism moth oxygen consumption physiology South Africa Animals Moths Animalia Insecta Invertebrata Lepidoptera Phacelia congesta Tineidae Sinclair B.J. Klok C.J. Chown S.L. Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
topic_facet |
carbon dioxide animal article body temperature comparative study energy metabolism freezing geography metabolism moth oxygen consumption physiology South Africa Animals Moths Animalia Insecta Invertebrata Lepidoptera Phacelia congesta Tineidae |
description |
Although general models of the processes involved in insect survival of freezing exist, there have been few studies directly investigating physiological processes during cooling, freezing and thawing, without which these models remain hypothetical. Here, we use open-flow respirometry to investigate the metabolism of the freeze-tolerant sub-Antarctic caterpillar Pringleophaga marioni Viette (Lepidoptera: Tineidae) during cooling, freezing and thawing and to compare animals exposed to non-lethal (-5.8°C) and lethal (-6.0°C, after which caterpillars are moribund for several days, and -18°C, after which caterpillars are completely unresponsive) freezing stress. We found a large decrease in metabolic rate (that is not associated with freezing) at -0.6±0.1°C and calculated a Q10 of 2.14×10 3 at this breakpoint. This breakpoint is coincident with the critical thermal minimum (CTmin) and is hypothesised to be a metabolic manifestation of the latter, possibly a failure of the Na+/K +-ATPase pump. This provides a plausible link between processes at the cellular level and observations of the action of the CTmin at tissue and whole-organism levels. Caterpillars froze at -4.6±0.1°C and had detectable metabolism when frozen. Post-thaw, metabolic rates were lower than pre-freering measurements. Post-thaw metabolic rates did not differ between temperatures that did and did not kill the caterpillars, which suggests that mortality may be a result of a breakdown in processes at the organismal, rather than cellular, level of organisation. Article |
format |
Article in Journal/Newspaper |
author |
Sinclair B.J. Klok C.J. Chown S.L. |
author_facet |
Sinclair B.J. Klok C.J. Chown S.L. |
author_sort |
Sinclair B.J. |
title |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_short |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_full |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_fullStr |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_full_unstemmed |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_sort |
metabolism of the sub-antarctic caterpillar pringleophaga marioni during cooling, freezing and thawing |
publishDate |
2004 |
url |
http://hdl.handle.net/10019.1/12952 https://doi.org/10.1242/jeb.00880 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Journal of Experimental Biology 207 8 220949 doi:10.1242/jeb.00880 http://hdl.handle.net/10019.1/12952 |
op_doi |
https://doi.org/10.1242/jeb.00880 |
container_title |
Journal of Experimental Biology |
container_volume |
207 |
container_issue |
8 |
container_start_page |
1287 |
op_container_end_page |
1294 |
_version_ |
1782328537739427840 |