Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method
Thesis (MSc)--Stellenbosch University, 2017. ENGLISH ABSTRACT: A method comprising an improved seawater collection protocol and subsequent Inductively Coupled Plasma Mass Spectrometry (ICP-MS) based analytical technique was validated through an intercalibration exercise performed with the University...
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ftunstellenbosch:oai:scholar.sun.ac.za:10019.1/101064 2023-11-12T04:01:33+01:00 Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method Cloete, Ryan Roychoudhury, Alakendra N. Stellenbosch University. Faculty of Science. Dept. of Earth Sciences. 2017-02-17T13:40:05Z 118 pages : illustrations application/pdf http://hdl.handle.net/10019.1/101064 en_ZA eng Stellenbosch : Stellenbosch University http://hdl.handle.net/10019.1/101064 Stellenbosch University Bioactive trace metals -- Southern Ocean Bioactive trace metals -- Analysis Copper concentrations -- Southern Ocean Zinc concentrations -- Southern Ocean Inductively Coupled Plasma Mass Spectrometry (ICP-MS) UCTD Thesis 2017 ftunstellenbosch 2023-10-22T07:35:02Z Thesis (MSc)--Stellenbosch University, 2017. ENGLISH ABSTRACT: A method comprising an improved seawater collection protocol and subsequent Inductively Coupled Plasma Mass Spectrometry (ICP-MS) based analytical technique was validated through an intercalibration exercise performed with the University of Plymouth (UK), multiple cross-over stations and analyses of certified reference materials (SAFe, GEOTRACES and NASS-5). The commercially available seaFAST-pico preconcentration module was employed for the simultaneous extraction of a suite of trace metals (Mn, Fe, Ni, Cu, Zn, Co, Cd and Pb) from their seawater matrix prior to ICP-MS analysis. Extremely low detection limits (< 0.228 nmol/kg) combined with low blank values ensured quantitive recovery on ICP-MS and minimal interferences arising from alkali and alkaline earth metals (Na, K, Mg and Ca) present in the saline matrix. The results of the certified reference materials were in excellent agreement with their corresponding consensus values and validated the methods precision and accuracy. During ICP-MS analysis, repeatability and reproducibility were monitored through analysis of an internal Stellenbosch University (SU) TM4 control and various commercially available quality controls, the results of which further confirmed a high level of precision. The distribution of Dissolved Copper (DCu) and Dissolved Zinc (DZn) was investigated in the Atlantic sector of the Southern Ocean. DCu displayed typical nutrient type behaviour reflected by sub-nanomolar surface concentrations increasing steadily until maximum observed concentrations of 2 – 3 nmol/kg in the Antarctic Bottom Waters (AABW). DZn concentrations ranged between approximately 1 and 12 nmol/kg and exhibited characteristic nutrient-type behaviour although intermediate and deepwater distributions were more conservative compared to DCu. Local subsurface minima coincided with elevated levels of chlorophyll-a (chl-a) indicating biological utilisation by phytoplankton in the euphotic zone. Remineralisation ... Thesis Antarc* Antarctic Southern Ocean Stellenbosch University: SUNScholar Research Repository Antarctic Southern Ocean The Antarctic |
institution |
Open Polar |
collection |
Stellenbosch University: SUNScholar Research Repository |
op_collection_id |
ftunstellenbosch |
language |
English |
topic |
Bioactive trace metals -- Southern Ocean Bioactive trace metals -- Analysis Copper concentrations -- Southern Ocean Zinc concentrations -- Southern Ocean Inductively Coupled Plasma Mass Spectrometry (ICP-MS) UCTD |
spellingShingle |
Bioactive trace metals -- Southern Ocean Bioactive trace metals -- Analysis Copper concentrations -- Southern Ocean Zinc concentrations -- Southern Ocean Inductively Coupled Plasma Mass Spectrometry (ICP-MS) UCTD Cloete, Ryan Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method |
topic_facet |
Bioactive trace metals -- Southern Ocean Bioactive trace metals -- Analysis Copper concentrations -- Southern Ocean Zinc concentrations -- Southern Ocean Inductively Coupled Plasma Mass Spectrometry (ICP-MS) UCTD |
description |
Thesis (MSc)--Stellenbosch University, 2017. ENGLISH ABSTRACT: A method comprising an improved seawater collection protocol and subsequent Inductively Coupled Plasma Mass Spectrometry (ICP-MS) based analytical technique was validated through an intercalibration exercise performed with the University of Plymouth (UK), multiple cross-over stations and analyses of certified reference materials (SAFe, GEOTRACES and NASS-5). The commercially available seaFAST-pico preconcentration module was employed for the simultaneous extraction of a suite of trace metals (Mn, Fe, Ni, Cu, Zn, Co, Cd and Pb) from their seawater matrix prior to ICP-MS analysis. Extremely low detection limits (< 0.228 nmol/kg) combined with low blank values ensured quantitive recovery on ICP-MS and minimal interferences arising from alkali and alkaline earth metals (Na, K, Mg and Ca) present in the saline matrix. The results of the certified reference materials were in excellent agreement with their corresponding consensus values and validated the methods precision and accuracy. During ICP-MS analysis, repeatability and reproducibility were monitored through analysis of an internal Stellenbosch University (SU) TM4 control and various commercially available quality controls, the results of which further confirmed a high level of precision. The distribution of Dissolved Copper (DCu) and Dissolved Zinc (DZn) was investigated in the Atlantic sector of the Southern Ocean. DCu displayed typical nutrient type behaviour reflected by sub-nanomolar surface concentrations increasing steadily until maximum observed concentrations of 2 – 3 nmol/kg in the Antarctic Bottom Waters (AABW). DZn concentrations ranged between approximately 1 and 12 nmol/kg and exhibited characteristic nutrient-type behaviour although intermediate and deepwater distributions were more conservative compared to DCu. Local subsurface minima coincided with elevated levels of chlorophyll-a (chl-a) indicating biological utilisation by phytoplankton in the euphotic zone. Remineralisation ... |
author2 |
Roychoudhury, Alakendra N. Stellenbosch University. Faculty of Science. Dept. of Earth Sciences. |
format |
Thesis |
author |
Cloete, Ryan |
author_facet |
Cloete, Ryan |
author_sort |
Cloete, Ryan |
title |
Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method |
title_short |
Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method |
title_full |
Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method |
title_fullStr |
Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method |
title_full_unstemmed |
Measurement of bioactive trace metals (Cu and Zn) in the Southern Ocean : validation of sampling protocol and ICP-MS based analytical method |
title_sort |
measurement of bioactive trace metals (cu and zn) in the southern ocean : validation of sampling protocol and icp-ms based analytical method |
publisher |
Stellenbosch : Stellenbosch University |
publishDate |
2017 |
url |
http://hdl.handle.net/10019.1/101064 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
http://hdl.handle.net/10019.1/101064 |
op_rights |
Stellenbosch University |
_version_ |
1782332814344060928 |