Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar)
The chemical signals in the sequential layers of fish otoliths have the potential to provide fisheries biologists with temporal and spatial details of migration which are difficult to obtain without expensive tracking methods. Signal resolution depends, however, on the extraction technique used. We...
Published in: | Rapid Communications in Mass Spectrometry |
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/comparison-of-secondary-ion-mass-spectrometry-and-micromillingcontinuous-flow-isotope-ratio-mass-spectrometry-techniques-used-to-acquire-intraotolith-delta-o18-values-of-wild-atlantic-salmon-salmo-salar(ac9560bf-0f5d-4f12-b19e-e1a0289b3c3d).html https://doi.org/10.1002/rcm.4646 http://www.scopus.com/inward/record.url?scp=77955411529&partnerID=8YFLogxK |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/ac9560bf-0f5d-4f12-b19e-e1a0289b3c3d 2023-05-15T15:31:44+02:00 Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) Hanson, N. N. Wurster, C. M. Todd, C. D. 2010-09 https://risweb.st-andrews.ac.uk/portal/en/researchoutput/comparison-of-secondary-ion-mass-spectrometry-and-micromillingcontinuous-flow-isotope-ratio-mass-spectrometry-techniques-used-to-acquire-intraotolith-delta-o18-values-of-wild-atlantic-salmon-salmo-salar(ac9560bf-0f5d-4f12-b19e-e1a0289b3c3d).html https://doi.org/10.1002/rcm.4646 http://www.scopus.com/inward/record.url?scp=77955411529&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Hanson , N N , Wurster , C M , Todd , C D & EIMF 2010 , ' Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) ' , Rapid Communications in Mass Spectrometry , vol. 24 , no. 17 , pp. 2491-2498 . https://doi.org/10.1002/rcm.4646 COD GADUS-MORHUA FISH OTOLITHS ONCORHYNCHUS-NERKA LIFE-HISTORY OXYGEN FRACTIONATION TEMPERATURE RESOLUTION RECONSTRUCTION DELTA-C-13 article 2010 ftunstandrewcris https://doi.org/10.1002/rcm.4646 2021-12-26T14:17:43Z The chemical signals in the sequential layers of fish otoliths have the potential to provide fisheries biologists with temporal and spatial details of migration which are difficult to obtain without expensive tracking methods. Signal resolution depends, however, on the extraction technique used. We compared the use of mechanical micromilling and continuous flow isotope ratio mass spectrometry (CF-IRMS) methods with secondary ion mass spectrometry (SIMS) to obtain delta O-18 profiles from otoliths of wild Atlantic salmon (Salmo salar) and used these to corroborate the time of freshwater emigration of the juvenile with macroscopic patterns within the otolith. Both techniques showed the transition occurring at the same visible feature on the otolith, allowing future analyses to easily identify the juvenile (freshwater) versus adult (marine) life-stages. However, SIMS showed a rapid and abrupt transition whereas micromilling provided a less distinct signal. The number of samples that could be obtained per unit area sampled using SIMS was 2 to 3 times greater than that when using micromilling/CF-IRMS although the delta O-18 values and analytical precisions (similar to 0.2 parts per thousand) of the two methods were comparable. In addition, SIMS delta O-18 results were used to compare otolith aragonite values with predicted values calculated using various isotope fractionation equations. Copyright (C) 2010 John Wiley & Sons, Ltd. Article in Journal/Newspaper Atlantic salmon Gadus morhua Salmo salar University of St Andrews: Research Portal Rapid Communications in Mass Spectrometry 24 17 2491 2498 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
COD GADUS-MORHUA FISH OTOLITHS ONCORHYNCHUS-NERKA LIFE-HISTORY OXYGEN FRACTIONATION TEMPERATURE RESOLUTION RECONSTRUCTION DELTA-C-13 |
spellingShingle |
COD GADUS-MORHUA FISH OTOLITHS ONCORHYNCHUS-NERKA LIFE-HISTORY OXYGEN FRACTIONATION TEMPERATURE RESOLUTION RECONSTRUCTION DELTA-C-13 Hanson, N. N. Wurster, C. M. Todd, C. D. Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) |
topic_facet |
COD GADUS-MORHUA FISH OTOLITHS ONCORHYNCHUS-NERKA LIFE-HISTORY OXYGEN FRACTIONATION TEMPERATURE RESOLUTION RECONSTRUCTION DELTA-C-13 |
description |
The chemical signals in the sequential layers of fish otoliths have the potential to provide fisheries biologists with temporal and spatial details of migration which are difficult to obtain without expensive tracking methods. Signal resolution depends, however, on the extraction technique used. We compared the use of mechanical micromilling and continuous flow isotope ratio mass spectrometry (CF-IRMS) methods with secondary ion mass spectrometry (SIMS) to obtain delta O-18 profiles from otoliths of wild Atlantic salmon (Salmo salar) and used these to corroborate the time of freshwater emigration of the juvenile with macroscopic patterns within the otolith. Both techniques showed the transition occurring at the same visible feature on the otolith, allowing future analyses to easily identify the juvenile (freshwater) versus adult (marine) life-stages. However, SIMS showed a rapid and abrupt transition whereas micromilling provided a less distinct signal. The number of samples that could be obtained per unit area sampled using SIMS was 2 to 3 times greater than that when using micromilling/CF-IRMS although the delta O-18 values and analytical precisions (similar to 0.2 parts per thousand) of the two methods were comparable. In addition, SIMS delta O-18 results were used to compare otolith aragonite values with predicted values calculated using various isotope fractionation equations. Copyright (C) 2010 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Hanson, N. N. Wurster, C. M. Todd, C. D. |
author_facet |
Hanson, N. N. Wurster, C. M. Todd, C. D. |
author_sort |
Hanson, N. N. |
title |
Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) |
title_short |
Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) |
title_full |
Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) |
title_fullStr |
Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) |
title_sort |
comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta o-18 values of wild atlantic salmon (salmo salar) |
publishDate |
2010 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/comparison-of-secondary-ion-mass-spectrometry-and-micromillingcontinuous-flow-isotope-ratio-mass-spectrometry-techniques-used-to-acquire-intraotolith-delta-o18-values-of-wild-atlantic-salmon-salmo-salar(ac9560bf-0f5d-4f12-b19e-e1a0289b3c3d).html https://doi.org/10.1002/rcm.4646 http://www.scopus.com/inward/record.url?scp=77955411529&partnerID=8YFLogxK |
genre |
Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
Atlantic salmon Gadus morhua Salmo salar |
op_source |
Hanson , N N , Wurster , C M , Todd , C D & EIMF 2010 , ' Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith delta O-18 values of wild Atlantic salmon (Salmo salar) ' , Rapid Communications in Mass Spectrometry , vol. 24 , no. 17 , pp. 2491-2498 . https://doi.org/10.1002/rcm.4646 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1002/rcm.4646 |
container_title |
Rapid Communications in Mass Spectrometry |
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24 |
container_issue |
17 |
container_start_page |
2491 |
op_container_end_page |
2498 |
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