Feeding increases MyoD and PCNA expression in myogenic progenitor cells of Notothenia coriiceps.

• Published in: Journal of Fish Biology
• Main Authors: Brodeur, JC, Peck, LS, Johnston, Ian Alistair
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2002
• Subjects: myogenic precursor; satellite cell; SDA; MyoD; myogenin; Antarctic fish; MUSCLE SATELLITE CELLS; SKELETAL-MUSCLE; PROTEIN-SYNTHESIS; CHANNEL CATFISH; DYNAMIC ACTION; GROWTH; DEATH; TEMPERATURE; RICHARDSON; Antarctic; The Antarctic; Antarc*
• Online Access: https://risweb.st-andrews.ac.uk/portal/en/researchoutput/feeding-increases-myod-and-pcna-expression-in-myogenic-progenitor-cells-of-notothenia-coriiceps(93d42ff3-2c5a-4ca3-b9b6-7d2ec2b70f58).html; https://doi.org/10.1006/jfbi.2002.2007; http://www.scopus.com/inward/record.url?scp=0036621982&partnerID=8YFLogxK

Oxygen consumption and ammonia excretion of the Antarctic fish Notothenia corikeps (18.4 cm L-F) increased respectively two and fourfold above fasting levels 24 h after feeding with a single meal of shrimps (5.5 to 7.5% of body mass), and remained elevated for 120 h. In fasted fish, c-met positive cells in the fast muscle represented 5.5% of the total number of myonuclei. The number of c-met positive cells staining for the proliferating cell nuclear antigen was increased by 60% both 24 and 96 h after the meal, while the number of cells expressing the myogenic transcription factor, MyoD, was increased by 20% after 24 h, and by 44% after 96 h. The total numbers of c-met positive cells and cells expressing myogenin were not significantly altered 96 h following feeding. The results are consistent with an activation of myogenic progenitor cells proliferation by feeding but suggest a relatively long cell cycle time. (C) 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.