Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.).
Potential molecular mechanisms regulating developmental plasticity to temperature were investigated in Atlantic salmon embryos ( Salmo salar L.). Six orthologues of the four myogenic regulatory factors ( MRFs: individually: smyf5, smyoD1a/1b/1c, smyoG and sMRF4), the master transcription factors reg...
Published in: | Journal of Experimental Biology |
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2007
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/temperature-influences-the-coordinated-expression-of-myogenic-regulatory-factors-during-embryonic-embryogenesis-in-atlantic-salmon-salmo-salar-l(6ad94a85-8494-4476-8128-1e3b027739d4).html https://doi.org/10.1242/jeb.006981 http://www.scopus.com/inward/record.url?scp=34548608759&partnerID=8YFLogxK |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/6ad94a85-8494-4476-8128-1e3b027739d4 2023-05-15T15:32:11+02:00 Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). Johnston, Ian Alistair Robb, D Johnston, I A 2007-08-15 https://risweb.st-andrews.ac.uk/portal/en/researchoutput/temperature-influences-the-coordinated-expression-of-myogenic-regulatory-factors-during-embryonic-embryogenesis-in-atlantic-salmon-salmo-salar-l(6ad94a85-8494-4476-8128-1e3b027739d4).html https://doi.org/10.1242/jeb.006981 http://www.scopus.com/inward/record.url?scp=34548608759&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Johnston , I A , Robb , D & Johnston , I A 2007 , ' Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). ' , Journal of Experimental Biology , vol. 210 , pp. 2781 - 2794 . https://doi.org/10.1242/jeb.006981 Salmo salar teleost fish myogenesis myogenic regulatory factors adaxial cells temperature developmental plasticity TROUT ONCORHYNCHUS-MYKISS MUSCLE-FIBER RECRUITMENT HERRING CLUPEA-HARENGUS IN-SITU HYBRIDIZATION SKELETAL-MUSCLE DIFFERENTIAL EXPRESSION MAXIMUM-LIKELIHOOD SEQUENCE ALIGNMENT SOMITE FORMATION article 2007 ftunstandrewcris https://doi.org/10.1242/jeb.006981 2021-12-26T14:15:07Z Potential molecular mechanisms regulating developmental plasticity to temperature were investigated in Atlantic salmon embryos ( Salmo salar L.). Six orthologues of the four myogenic regulatory factors ( MRFs: individually: smyf5, smyoD1a/1b/1c, smyoG and sMRF4), the master transcription factors regulating vertebrate myogenesis, were characterised at the mRNA/genomic level. In situ hybridisation was performed with specific cRNA probes to determine the expression patterns of each gene during embryonic myogenesis. To place the MRF data in the context of known muscle fibre differentiation events, the expression of slow myosin light chain-1 and Pax7 were also investigated. Adaxial myoblasts expressed smyoD1a prior to and during somitogenesis followed by smyoD1c ( 20-somite stage, ss), and sMRF4 ( 25-30 ss), before spreading laterally across the myotome, followed closely by the adaxial cells. Smyf5 was detected prior to somitogenesis, but not in the adaxial cells in contrast to other teleosts studied. The expression domains of smyf5, smyoD1b and smyoG were not confined to the ssmlc1 expression field, indicating a role in fast muscle myogenesis. From the end of segmentation, each MRF was expressed to a greater or lesser extent in zones of new muscle fibre production, the precursor cells for which probably originated from the Pax7 expressing cell layer external to the single layer of s-smlc1(+) fibres. SmyoD1a and smyoG showed similar expression patterns with respect to somite stage at three different temperatures investigated ( 2 C, 5 C and 8 C) in spite of different rates of somite formation ( one somite added each 5 h, 8 h and 15 h at 8 C, 5 C and 2 C, respectively). In contrast, the expression of smyf5, sMRF4 and s-smlc1 was retarded with respect to somite stage at 2 C compared to 8 C, potentially resulting in heterochronies in downstream pathways influencing later muscle phenotype. Article in Journal/Newspaper Atlantic salmon Salmo salar University of St Andrews: Research Portal Journal of Experimental Biology 210 16 2781 2794 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
Salmo salar teleost fish myogenesis myogenic regulatory factors adaxial cells temperature developmental plasticity TROUT ONCORHYNCHUS-MYKISS MUSCLE-FIBER RECRUITMENT HERRING CLUPEA-HARENGUS IN-SITU HYBRIDIZATION SKELETAL-MUSCLE DIFFERENTIAL EXPRESSION MAXIMUM-LIKELIHOOD SEQUENCE ALIGNMENT SOMITE FORMATION |
spellingShingle |
Salmo salar teleost fish myogenesis myogenic regulatory factors adaxial cells temperature developmental plasticity TROUT ONCORHYNCHUS-MYKISS MUSCLE-FIBER RECRUITMENT HERRING CLUPEA-HARENGUS IN-SITU HYBRIDIZATION SKELETAL-MUSCLE DIFFERENTIAL EXPRESSION MAXIMUM-LIKELIHOOD SEQUENCE ALIGNMENT SOMITE FORMATION Johnston, Ian Alistair Robb, D Johnston, I A Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). |
topic_facet |
Salmo salar teleost fish myogenesis myogenic regulatory factors adaxial cells temperature developmental plasticity TROUT ONCORHYNCHUS-MYKISS MUSCLE-FIBER RECRUITMENT HERRING CLUPEA-HARENGUS IN-SITU HYBRIDIZATION SKELETAL-MUSCLE DIFFERENTIAL EXPRESSION MAXIMUM-LIKELIHOOD SEQUENCE ALIGNMENT SOMITE FORMATION |
description |
Potential molecular mechanisms regulating developmental plasticity to temperature were investigated in Atlantic salmon embryos ( Salmo salar L.). Six orthologues of the four myogenic regulatory factors ( MRFs: individually: smyf5, smyoD1a/1b/1c, smyoG and sMRF4), the master transcription factors regulating vertebrate myogenesis, were characterised at the mRNA/genomic level. In situ hybridisation was performed with specific cRNA probes to determine the expression patterns of each gene during embryonic myogenesis. To place the MRF data in the context of known muscle fibre differentiation events, the expression of slow myosin light chain-1 and Pax7 were also investigated. Adaxial myoblasts expressed smyoD1a prior to and during somitogenesis followed by smyoD1c ( 20-somite stage, ss), and sMRF4 ( 25-30 ss), before spreading laterally across the myotome, followed closely by the adaxial cells. Smyf5 was detected prior to somitogenesis, but not in the adaxial cells in contrast to other teleosts studied. The expression domains of smyf5, smyoD1b and smyoG were not confined to the ssmlc1 expression field, indicating a role in fast muscle myogenesis. From the end of segmentation, each MRF was expressed to a greater or lesser extent in zones of new muscle fibre production, the precursor cells for which probably originated from the Pax7 expressing cell layer external to the single layer of s-smlc1(+) fibres. SmyoD1a and smyoG showed similar expression patterns with respect to somite stage at three different temperatures investigated ( 2 C, 5 C and 8 C) in spite of different rates of somite formation ( one somite added each 5 h, 8 h and 15 h at 8 C, 5 C and 2 C, respectively). In contrast, the expression of smyf5, sMRF4 and s-smlc1 was retarded with respect to somite stage at 2 C compared to 8 C, potentially resulting in heterochronies in downstream pathways influencing later muscle phenotype. |
format |
Article in Journal/Newspaper |
author |
Johnston, Ian Alistair Robb, D Johnston, I A |
author_facet |
Johnston, Ian Alistair Robb, D Johnston, I A |
author_sort |
Johnston, Ian Alistair |
title |
Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). |
title_short |
Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). |
title_full |
Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). |
title_fullStr |
Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). |
title_full_unstemmed |
Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). |
title_sort |
temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in atlantic salmon (salmo salar l.). |
publishDate |
2007 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/temperature-influences-the-coordinated-expression-of-myogenic-regulatory-factors-during-embryonic-embryogenesis-in-atlantic-salmon-salmo-salar-l(6ad94a85-8494-4476-8128-1e3b027739d4).html https://doi.org/10.1242/jeb.006981 http://www.scopus.com/inward/record.url?scp=34548608759&partnerID=8YFLogxK |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Johnston , I A , Robb , D & Johnston , I A 2007 , ' Temperature influences the co-ordinated expression of myogenic regulatory factors during embryonic embryogenesis in Atlantic salmon (Salmo salar L.). ' , Journal of Experimental Biology , vol. 210 , pp. 2781 - 2794 . https://doi.org/10.1242/jeb.006981 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1242/jeb.006981 |
container_title |
Journal of Experimental Biology |
container_volume |
210 |
container_issue |
16 |
container_start_page |
2781 |
op_container_end_page |
2794 |
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1766362680383242240 |