Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.).
Common carp (Cyprinus carpio L.) were reared from hatching until 61 mm total length (TL) at 21 degreesC. At 14 weeks and 20 weeks post-hatch, corresponding to initial lengths of 30 mm and 44 mm respectively, fish were acclimated to 10 degreesC using a rate of cooling of 1 degreesC per day. A statist...
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/plasticity-of-myosin-heavy-chain-expression-with-temperature-acclimation-is-gradually-acquired-during-ontogeny-in-the-common-carp-cyprinus-carpio-l(5a1dd3ee-80d4-4cc9-9119-bf450ef3cd27).html http://www.scopus.com/inward/record.url?scp=0035008067&partnerID=8YFLogxK |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/5a1dd3ee-80d4-4cc9-9119-bf450ef3cd27 2023-05-15T14:30:11+02:00 Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). Cole, NJ Johnston, Ian Alistair 2001-05 https://risweb.st-andrews.ac.uk/portal/en/researchoutput/plasticity-of-myosin-heavy-chain-expression-with-temperature-acclimation-is-gradually-acquired-during-ontogeny-in-the-common-carp-cyprinus-carpio-l(5a1dd3ee-80d4-4cc9-9119-bf450ef3cd27).html http://www.scopus.com/inward/record.url?scp=0035008067&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Cole , NJ & Johnston , I A 2001 , ' Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). ' , Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology , vol. 171 , pp. 321-326 . muscle temperature acclimation ontogeny myosin heavy chain Cyprinus carpio MYOFIBRILLAR ATPASE ACTIVITY FAST SKELETAL-MUSCLE SALVELINUS-ALPINUS L THERMAL-ACCLIMATION BODY-TEMPERATURE MULTIGENE FAMILY CLUPEA-HARENGUS GENE-EXPRESSION ARCTIC CHARR FIBER TYPES article 2001 ftunstandrewcris 2021-12-26T14:10:39Z Common carp (Cyprinus carpio L.) were reared from hatching until 61 mm total length (TL) at 21 degreesC. At 14 weeks and 20 weeks post-hatch, corresponding to initial lengths of 30 mm and 44 mm respectively, fish were acclimated to 10 degreesC using a rate of cooling of 1 degreesC per day. A statistical model was used to compare the time course in the change of white muscle myofibrillar ATPase activity with temperature acclimation. The myosin heavy chain (MHC) composition of white muscle myofibrils was investigated using peptide mapping. A significant increase in myofibrillar ATPase activity was observed after 2-3 weeks in the 44 mm group, but not until 4-5 weeks in the 30 mm group, when they had reached 37 mm TL. The MHC banding pattern of 120 mm TL fish acclimated to 10 degreesC or 21 degreesC for a minimum of 6 weeks were distinct from each other. The MHC peptide map characteristic of 10-degreesC-acclimated fish was not observed in individuals less than 37 mm length. We therefore conclude that the capacity to alter the composition and properties of myofibrils with cold acclimation is acquired in juvenile carp at around 37 mm TL. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus University of St Andrews: Research Portal Arctic |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
muscle temperature acclimation ontogeny myosin heavy chain Cyprinus carpio MYOFIBRILLAR ATPASE ACTIVITY FAST SKELETAL-MUSCLE SALVELINUS-ALPINUS L THERMAL-ACCLIMATION BODY-TEMPERATURE MULTIGENE FAMILY CLUPEA-HARENGUS GENE-EXPRESSION ARCTIC CHARR FIBER TYPES |
spellingShingle |
muscle temperature acclimation ontogeny myosin heavy chain Cyprinus carpio MYOFIBRILLAR ATPASE ACTIVITY FAST SKELETAL-MUSCLE SALVELINUS-ALPINUS L THERMAL-ACCLIMATION BODY-TEMPERATURE MULTIGENE FAMILY CLUPEA-HARENGUS GENE-EXPRESSION ARCTIC CHARR FIBER TYPES Cole, NJ Johnston, Ian Alistair Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). |
topic_facet |
muscle temperature acclimation ontogeny myosin heavy chain Cyprinus carpio MYOFIBRILLAR ATPASE ACTIVITY FAST SKELETAL-MUSCLE SALVELINUS-ALPINUS L THERMAL-ACCLIMATION BODY-TEMPERATURE MULTIGENE FAMILY CLUPEA-HARENGUS GENE-EXPRESSION ARCTIC CHARR FIBER TYPES |
description |
Common carp (Cyprinus carpio L.) were reared from hatching until 61 mm total length (TL) at 21 degreesC. At 14 weeks and 20 weeks post-hatch, corresponding to initial lengths of 30 mm and 44 mm respectively, fish were acclimated to 10 degreesC using a rate of cooling of 1 degreesC per day. A statistical model was used to compare the time course in the change of white muscle myofibrillar ATPase activity with temperature acclimation. The myosin heavy chain (MHC) composition of white muscle myofibrils was investigated using peptide mapping. A significant increase in myofibrillar ATPase activity was observed after 2-3 weeks in the 44 mm group, but not until 4-5 weeks in the 30 mm group, when they had reached 37 mm TL. The MHC banding pattern of 120 mm TL fish acclimated to 10 degreesC or 21 degreesC for a minimum of 6 weeks were distinct from each other. The MHC peptide map characteristic of 10-degreesC-acclimated fish was not observed in individuals less than 37 mm length. We therefore conclude that the capacity to alter the composition and properties of myofibrils with cold acclimation is acquired in juvenile carp at around 37 mm TL. |
format |
Article in Journal/Newspaper |
author |
Cole, NJ Johnston, Ian Alistair |
author_facet |
Cole, NJ Johnston, Ian Alistair |
author_sort |
Cole, NJ |
title |
Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). |
title_short |
Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). |
title_full |
Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). |
title_fullStr |
Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). |
title_full_unstemmed |
Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). |
title_sort |
plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (cyprinus carpio l.). |
publishDate |
2001 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/plasticity-of-myosin-heavy-chain-expression-with-temperature-acclimation-is-gradually-acquired-during-ontogeny-in-the-common-carp-cyprinus-carpio-l(5a1dd3ee-80d4-4cc9-9119-bf450ef3cd27).html http://www.scopus.com/inward/record.url?scp=0035008067&partnerID=8YFLogxK |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
Cole , NJ & Johnston , I A 2001 , ' Plasticity of myosin heavy chain expression with temperature acclimation is gradually acquired during ontogeny in the common carp (Cyprinus carpio L.). ' , Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology , vol. 171 , pp. 321-326 . |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1766304078728527872 |