Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes
This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term up-...
Published in: | Developmental & Comparative Immunology |
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2005
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/realtime-pcr-quantification-of-the-in-vitro-effects-of-crustacean-immunostimulants-on-gene-expression-in-lobster-homarus-gammarus-granular-haemocytes(543a77a1-1d7e-45a7-a6b0-41dbcd23e113).html https://doi.org/10.1016/j.dci.2004.05.006 http://www.scopus.com/inward/record.url?scp=4344591431&partnerID=8YFLogxK |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/543a77a1-1d7e-45a7-a6b0-41dbcd23e113 2023-05-15T16:08:49+02:00 Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes Hauton, Christopher Hammond, JA Smith, Valerie Jane 2005-01 https://risweb.st-andrews.ac.uk/portal/en/researchoutput/realtime-pcr-quantification-of-the-in-vitro-effects-of-crustacean-immunostimulants-on-gene-expression-in-lobster-homarus-gammarus-granular-haemocytes(543a77a1-1d7e-45a7-a6b0-41dbcd23e113).html https://doi.org/10.1016/j.dci.2004.05.006 http://www.scopus.com/inward/record.url?scp=4344591431&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Hauton , C , Hammond , JA & Smith , V J 2005 , ' Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes ' , Developmental and Comparative Immunology , vol. 29 , no. 1 , pp. 33-42 . https://doi.org/10.1016/j.dci.2004.05.006 real-time PCR immunostimulants prophenoloxidase beta-1,3-glucan binding protein lobster Homarus gammarus granulocytes PROPHENOLOXIDASE-ACTIVATING SYSTEM CRAYFISH PACIFASTACUS-LENIUSCULUS BETA-1,3-GLUCAN BINDING-PROTEIN FRESH-WATER CRAYFISH MESSENGER-RNA LEVELS BLACK TIGER SHRIMP PENAEUS-MONODON RT-PCR LIPOPOLYSACCHARIDE AQUACULTURE article 2005 ftunstandrewcris https://doi.org/10.1016/j.dci.2004.05.006 2021-12-26T14:12:56Z This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term up-regulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), beta-1,3-glucan binding protein (betaGBP) and beta-actin (beta-act) in the lobster. These sequences were used to design TaqMan(TM) primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed. (C) 2004 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper European lobster Homarus gammarus University of St Andrews: Research Portal Developmental & Comparative Immunology 29 1 33 42 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
real-time PCR immunostimulants prophenoloxidase beta-1,3-glucan binding protein lobster Homarus gammarus granulocytes PROPHENOLOXIDASE-ACTIVATING SYSTEM CRAYFISH PACIFASTACUS-LENIUSCULUS BETA-1,3-GLUCAN BINDING-PROTEIN FRESH-WATER CRAYFISH MESSENGER-RNA LEVELS BLACK TIGER SHRIMP PENAEUS-MONODON RT-PCR LIPOPOLYSACCHARIDE AQUACULTURE |
spellingShingle |
real-time PCR immunostimulants prophenoloxidase beta-1,3-glucan binding protein lobster Homarus gammarus granulocytes PROPHENOLOXIDASE-ACTIVATING SYSTEM CRAYFISH PACIFASTACUS-LENIUSCULUS BETA-1,3-GLUCAN BINDING-PROTEIN FRESH-WATER CRAYFISH MESSENGER-RNA LEVELS BLACK TIGER SHRIMP PENAEUS-MONODON RT-PCR LIPOPOLYSACCHARIDE AQUACULTURE Hauton, Christopher Hammond, JA Smith, Valerie Jane Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes |
topic_facet |
real-time PCR immunostimulants prophenoloxidase beta-1,3-glucan binding protein lobster Homarus gammarus granulocytes PROPHENOLOXIDASE-ACTIVATING SYSTEM CRAYFISH PACIFASTACUS-LENIUSCULUS BETA-1,3-GLUCAN BINDING-PROTEIN FRESH-WATER CRAYFISH MESSENGER-RNA LEVELS BLACK TIGER SHRIMP PENAEUS-MONODON RT-PCR LIPOPOLYSACCHARIDE AQUACULTURE |
description |
This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term up-regulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), beta-1,3-glucan binding protein (betaGBP) and beta-actin (beta-act) in the lobster. These sequences were used to design TaqMan(TM) primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed. (C) 2004 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Hauton, Christopher Hammond, JA Smith, Valerie Jane |
author_facet |
Hauton, Christopher Hammond, JA Smith, Valerie Jane |
author_sort |
Hauton, Christopher |
title |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes |
title_short |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes |
title_full |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes |
title_fullStr |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes |
title_full_unstemmed |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes |
title_sort |
real-time pcr quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( homarus gammarus ) granular haemocytes |
publishDate |
2005 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/realtime-pcr-quantification-of-the-in-vitro-effects-of-crustacean-immunostimulants-on-gene-expression-in-lobster-homarus-gammarus-granular-haemocytes(543a77a1-1d7e-45a7-a6b0-41dbcd23e113).html https://doi.org/10.1016/j.dci.2004.05.006 http://www.scopus.com/inward/record.url?scp=4344591431&partnerID=8YFLogxK |
genre |
European lobster Homarus gammarus |
genre_facet |
European lobster Homarus gammarus |
op_source |
Hauton , C , Hammond , JA & Smith , V J 2005 , ' Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster ( Homarus gammarus ) granular haemocytes ' , Developmental and Comparative Immunology , vol. 29 , no. 1 , pp. 33-42 . https://doi.org/10.1016/j.dci.2004.05.006 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.dci.2004.05.006 |
container_title |
Developmental & Comparative Immunology |
container_volume |
29 |
container_issue |
1 |
container_start_page |
33 |
op_container_end_page |
42 |
_version_ |
1766404829579575296 |