Cortisol regulates eel (Anguilla anguilla) aquaporin 3 (AQP3) mRNA expression levels in gill

• Published in: General and Comparative Endocrinology
• Main Authors: Cutler, C. P., Phillips, C., Hazon, N., Cramb, G.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2007
• Subjects: aquaporin 3; cortisol; gill; intestine; mRNA expression; EUROPEAN EEL; WATER CHANNELS; SEAWATER ACCLIMATION; NA,K-ATPASE; CHLORIDE; CELLS; FISH; RAT; Anguilla anguilla
• Online Access: https://risweb.st-andrews.ac.uk/portal/en/researchoutput/cortisol-regulates-eel-anguilla-anguilla-aquaporin-3-aqp3-mrna-expression-levels-in-gill(2c95452c-8e31-4336-9f5e-72a242345635).html; https://doi.org/10.1016/j.ygcen.2007.01.031; http://www.scopus.com/inward/record.url?scp=34250688746&partnerID=8YFLogxK

Previous studies in eel (Anguilla anguilla) gill have shown that the expression of the aquaporin 3 (AQP3) water and small solute channel is dramatically decreased (mRNA abundance decreased by up to 97%) when these euryhaline fish are acclimated from freshwater (FW) to seawater (SW). However, AQP3 mRNA expression levels in the intestine following SW-acclimation do not change. The SW-acclimating corticosteroid hormone, cortisol has previously been shown to regulate the expression of aquaporins (particularly AQP1) in eel osmoregulatory tissues in a tissue-specific and isoform-specific fashion. AQP1 is up-regulated in intestine and oesophagus, but down-regulated in kidney, following SW-acclimation in these fish. This study extends knowledge of the regulation of aquaporin expression by cortisol in the eel and shows that elevated levels of this hormone down-regulate AQP3 mRNA expression in the gill in a similar manner to SW-acclimation. However, the smaller magnitude of the changes in branchial AQP3 expression induced by cortisol-infusion (around a 60% decrease), in comparison to those occurring following SW-acclimation, suggest that other factors must also contribute to AQP3 down-regulation. In a similar fashion to the regulation of AQP1 by cortisol, changes in AQP3 expression following hormone infusion appear to be tissue-specific, as little effect was seen on the level of AQP3 expression in the intestine. Again the apparent lack of change in intestinal AQP3 expression following cortisol-infusion mimicked the invariant level of intestinal AQP3 mRNA abundance following SW-acclimation. (c) 2007 Elsevier Inc. All rights reserved.