Determination of steroid hormones in whale blow: It is possible
The feasibility of using analysis of hormone content of whale blow samples to assess reproductive function is addressed. A suitable collection method and analytical technique using liquid chromatography-mass spectrometry (LC-MS) has been developed. Blow samples were collected opportunistically from...
Published in: | Marine Mammal Science |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2009
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/determination-of-steroid-hormones-in-whale-blow-it-is-possible(2c0ef6ed-310e-4e5f-bda4-22f907f97f88).html https://doi.org/10.1111/j.1748-7692.2008.00277.x http://www.scopus.com/inward/record.url?scp=67849114531&partnerID=8YFLogxK |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/2c0ef6ed-310e-4e5f-bda4-22f907f97f88 2023-05-15T16:08:15+02:00 Determination of steroid hormones in whale blow: It is possible Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, Patrick Nowacek, D. 2009-07 https://risweb.st-andrews.ac.uk/portal/en/researchoutput/determination-of-steroid-hormones-in-whale-blow-it-is-possible(2c0ef6ed-310e-4e5f-bda4-22f907f97f88).html https://doi.org/10.1111/j.1748-7692.2008.00277.x http://www.scopus.com/inward/record.url?scp=67849114531&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Hogg , C J , Rogers , T L , Shorter , A , Barton , K , Miller , P & Nowacek , D 2009 , ' Determination of steroid hormones in whale blow: It is possible ' , Marine Mammal Science , vol. 25 , no. 3 , pp. 605-618 . https://doi.org/10.1111/j.1748-7692.2008.00277.x blow whale testosterone progesterone BOTTLE-NOSED DOLPHINS ATLANTIC RIGHT WHALES RAT NASAL-MUCOSA EUBALAENA-GLACIALIS KILLER WHALES MONACHUS-SCHAUINSLANDI ADRENAL-FUNCTION ESTROUS-CYCLE SALIVA article 2009 ftunstandrewcris https://doi.org/10.1111/j.1748-7692.2008.00277.x 2022-10-13T15:25:27Z The feasibility of using analysis of hormone content of whale blow samples to assess reproductive function is addressed. A suitable collection method and analytical technique using liquid chromatography-mass spectrometry (LC-MS) has been developed. Blow samples were collected opportunistically from free-ranging humpback whales (Megaptera novaeangliae) (n = 35) and North Atlantic right whales (Eubalaena glacialis) (n = 18) using a 13-m carbon fiber pole with a collection device. Samples were analyzed for the presence of testosterone and progesterone using a 55% isocratic gradient with LC-MS. Testosterone was detected in four humpback whale samples and eight northern right whale samples. Progesterone was detected in seven humpback whale samples and eight northern right whale samples. This is the first documented use of lung mucosa to determine the presence of reproductive hormones in any free-swimming cetacean and may provide a novel non-invasive technique to quantify the hormonal condition of free-swimming animals that spend brief periods of time at the water's surface. Article in Journal/Newspaper Eubalaena glacialis Humpback Whale Megaptera novaeangliae North Atlantic University of St Andrews: Research Portal Marine Mammal Science 25 3 605 618 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
blow whale testosterone progesterone BOTTLE-NOSED DOLPHINS ATLANTIC RIGHT WHALES RAT NASAL-MUCOSA EUBALAENA-GLACIALIS KILLER WHALES MONACHUS-SCHAUINSLANDI ADRENAL-FUNCTION ESTROUS-CYCLE SALIVA |
spellingShingle |
blow whale testosterone progesterone BOTTLE-NOSED DOLPHINS ATLANTIC RIGHT WHALES RAT NASAL-MUCOSA EUBALAENA-GLACIALIS KILLER WHALES MONACHUS-SCHAUINSLANDI ADRENAL-FUNCTION ESTROUS-CYCLE SALIVA Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, Patrick Nowacek, D. Determination of steroid hormones in whale blow: It is possible |
topic_facet |
blow whale testosterone progesterone BOTTLE-NOSED DOLPHINS ATLANTIC RIGHT WHALES RAT NASAL-MUCOSA EUBALAENA-GLACIALIS KILLER WHALES MONACHUS-SCHAUINSLANDI ADRENAL-FUNCTION ESTROUS-CYCLE SALIVA |
description |
The feasibility of using analysis of hormone content of whale blow samples to assess reproductive function is addressed. A suitable collection method and analytical technique using liquid chromatography-mass spectrometry (LC-MS) has been developed. Blow samples were collected opportunistically from free-ranging humpback whales (Megaptera novaeangliae) (n = 35) and North Atlantic right whales (Eubalaena glacialis) (n = 18) using a 13-m carbon fiber pole with a collection device. Samples were analyzed for the presence of testosterone and progesterone using a 55% isocratic gradient with LC-MS. Testosterone was detected in four humpback whale samples and eight northern right whale samples. Progesterone was detected in seven humpback whale samples and eight northern right whale samples. This is the first documented use of lung mucosa to determine the presence of reproductive hormones in any free-swimming cetacean and may provide a novel non-invasive technique to quantify the hormonal condition of free-swimming animals that spend brief periods of time at the water's surface. |
format |
Article in Journal/Newspaper |
author |
Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, Patrick Nowacek, D. |
author_facet |
Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, Patrick Nowacek, D. |
author_sort |
Hogg, C. J. |
title |
Determination of steroid hormones in whale blow: It is possible |
title_short |
Determination of steroid hormones in whale blow: It is possible |
title_full |
Determination of steroid hormones in whale blow: It is possible |
title_fullStr |
Determination of steroid hormones in whale blow: It is possible |
title_full_unstemmed |
Determination of steroid hormones in whale blow: It is possible |
title_sort |
determination of steroid hormones in whale blow: it is possible |
publishDate |
2009 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/determination-of-steroid-hormones-in-whale-blow-it-is-possible(2c0ef6ed-310e-4e5f-bda4-22f907f97f88).html https://doi.org/10.1111/j.1748-7692.2008.00277.x http://www.scopus.com/inward/record.url?scp=67849114531&partnerID=8YFLogxK |
genre |
Eubalaena glacialis Humpback Whale Megaptera novaeangliae North Atlantic |
genre_facet |
Eubalaena glacialis Humpback Whale Megaptera novaeangliae North Atlantic |
op_source |
Hogg , C J , Rogers , T L , Shorter , A , Barton , K , Miller , P & Nowacek , D 2009 , ' Determination of steroid hormones in whale blow: It is possible ' , Marine Mammal Science , vol. 25 , no. 3 , pp. 605-618 . https://doi.org/10.1111/j.1748-7692.2008.00277.x |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1748-7692.2008.00277.x |
container_title |
Marine Mammal Science |
container_volume |
25 |
container_issue |
3 |
container_start_page |
605 |
op_container_end_page |
618 |
_version_ |
1766404317560963072 |