Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ).
We have investigated the interactions between dietary vitamin C levels (at 33, 79, 135, and 424 mg kg(-1) of wet mass feed) and growth rate on the collagen and cross-link contents of fast muscle in farmed juvenile Atlantic salmon (Salmo salar L.). The growth rate was measured over an 11 week period...
Published in: | Journal of Agricultural and Food Chemistry |
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2007
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/investigations-on-the-effects-of-growth-rate-and-dietary-vitamin-c-on-skeletal-muscle-collagen-and-hydroxylysyl-pyridinoline-crosslink-concentration-in-farmed-atlantic-salmon-salmo-salar(210d55a0-378b-4a94-9f4c-408a4b1c81e6).html https://doi.org/10.1021/jf062251i http://www.scopus.com/inward/record.url?scp=34047229537&partnerID=8YFLogxK |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/210d55a0-378b-4a94-9f4c-408a4b1c81e6 2023-05-15T15:31:37+02:00 Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). Li, X J Bickerdike, R Nickell, D Campbell, P Dingwall, A Johnston, Ian Alistair 2007-01-24 https://risweb.st-andrews.ac.uk/portal/en/researchoutput/investigations-on-the-effects-of-growth-rate-and-dietary-vitamin-c-on-skeletal-muscle-collagen-and-hydroxylysyl-pyridinoline-crosslink-concentration-in-farmed-atlantic-salmon-salmo-salar(210d55a0-378b-4a94-9f4c-408a4b1c81e6).html https://doi.org/10.1021/jf062251i http://www.scopus.com/inward/record.url?scp=34047229537&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Li , X J , Bickerdike , R , Nickell , D , Campbell , P , Dingwall , A & Johnston , I A 2007 , ' Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). ' , Journal of Agricultural and Food Chemistry , vol. 55 , no. 2 , pp. 510-515 . https://doi.org/10.1021/jf062251i vitamin C collagen hydroxyproline cross-links fish muscle growth farmed Atlantic salmon texture flesh quality PERFORMANCE LIQUID-CHROMATOGRAPHY TROUT ONCORHYNCHUS-MYKISS L-ASCORBIC-ACID RAINBOW-TROUT CHANNEL CATFISH ICTALURUS-PUNCTATUS IMMUNE-RESPONSES BIOSYNTHESIS DIFFERENCE DISORDERS article 2007 ftunstandrewcris https://doi.org/10.1021/jf062251i 2021-12-26T14:13:56Z We have investigated the interactions between dietary vitamin C levels (at 33, 79, 135, and 424 mg kg(-1) of wet mass feed) and growth rate on the collagen and cross-link contents of fast muscle in farmed juvenile Atlantic salmon (Salmo salar L.). The growth rate was measured over an 11 week period using the thermal growth coefficient (TGC). Alkaline-soluble (0.1 M NaOH) (a-s) hydroxyproline (HYP) and alkaline-insoluble (i-s) HYP were determined as a measure of collagen content and hydroxylysyl pyridinoline (PYD) as a measure of mature collagen cross-link concentration. There was a similar to 5-fold increase in muscle vitamin C concentration at similar feed conversion ratios (similar to 0.82) as dietary vitamin C levels increased from 39 to 424 mg kg(-1) of wet mass feed. However, even the lowest dietary vitamin C was sufficient for normal skeletal development and growth. The lowest dietary vitamin C level tested resulted in a similar to 27% decrease in the a-sHYP concentration relative to the other diets, whereas there was no significant effect of vitamin C on the i-sHYP and PYD concentrations. ANOVA revealed no significant interaction between vitamin C and growth rate, whereas the covariate TGC was significant for i-sHYP and PYD but not for a-sHYP. Pyridinoline cross-link and i-s HYP concentrations were 11.1 and 7.7% lower, respectively, in high (TGC > 3.9) mass than low (TGC < 3.9) growth rate fish. These small differences in collagen cross-linking were associated with a 15.6% decrease in fillet firmness measured with an instrumental texture analyzer. It was concluded that for healthy juvenile salmon reared under controlled growth conditions, the dietary vitamin C inclusion of 79 mg kg(-1) of wet mass feed was sufficient to produce the required synthesis of soluble muscle collagen. Furthermore, post-translational modifications of the collagen leading to cross-linking showed a small decrease with increasing growth rate but was independent of vitamin C concentration in the diet at these levels. Article in Journal/Newspaper Atlantic salmon Salmo salar University of St Andrews: Research Portal Journal of Agricultural and Food Chemistry 55 2 510 515 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
vitamin C collagen hydroxyproline cross-links fish muscle growth farmed Atlantic salmon texture flesh quality PERFORMANCE LIQUID-CHROMATOGRAPHY TROUT ONCORHYNCHUS-MYKISS L-ASCORBIC-ACID RAINBOW-TROUT CHANNEL CATFISH ICTALURUS-PUNCTATUS IMMUNE-RESPONSES BIOSYNTHESIS DIFFERENCE DISORDERS |
spellingShingle |
vitamin C collagen hydroxyproline cross-links fish muscle growth farmed Atlantic salmon texture flesh quality PERFORMANCE LIQUID-CHROMATOGRAPHY TROUT ONCORHYNCHUS-MYKISS L-ASCORBIC-ACID RAINBOW-TROUT CHANNEL CATFISH ICTALURUS-PUNCTATUS IMMUNE-RESPONSES BIOSYNTHESIS DIFFERENCE DISORDERS Li, X J Bickerdike, R Nickell, D Campbell, P Dingwall, A Johnston, Ian Alistair Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). |
topic_facet |
vitamin C collagen hydroxyproline cross-links fish muscle growth farmed Atlantic salmon texture flesh quality PERFORMANCE LIQUID-CHROMATOGRAPHY TROUT ONCORHYNCHUS-MYKISS L-ASCORBIC-ACID RAINBOW-TROUT CHANNEL CATFISH ICTALURUS-PUNCTATUS IMMUNE-RESPONSES BIOSYNTHESIS DIFFERENCE DISORDERS |
description |
We have investigated the interactions between dietary vitamin C levels (at 33, 79, 135, and 424 mg kg(-1) of wet mass feed) and growth rate on the collagen and cross-link contents of fast muscle in farmed juvenile Atlantic salmon (Salmo salar L.). The growth rate was measured over an 11 week period using the thermal growth coefficient (TGC). Alkaline-soluble (0.1 M NaOH) (a-s) hydroxyproline (HYP) and alkaline-insoluble (i-s) HYP were determined as a measure of collagen content and hydroxylysyl pyridinoline (PYD) as a measure of mature collagen cross-link concentration. There was a similar to 5-fold increase in muscle vitamin C concentration at similar feed conversion ratios (similar to 0.82) as dietary vitamin C levels increased from 39 to 424 mg kg(-1) of wet mass feed. However, even the lowest dietary vitamin C was sufficient for normal skeletal development and growth. The lowest dietary vitamin C level tested resulted in a similar to 27% decrease in the a-sHYP concentration relative to the other diets, whereas there was no significant effect of vitamin C on the i-sHYP and PYD concentrations. ANOVA revealed no significant interaction between vitamin C and growth rate, whereas the covariate TGC was significant for i-sHYP and PYD but not for a-sHYP. Pyridinoline cross-link and i-s HYP concentrations were 11.1 and 7.7% lower, respectively, in high (TGC > 3.9) mass than low (TGC < 3.9) growth rate fish. These small differences in collagen cross-linking were associated with a 15.6% decrease in fillet firmness measured with an instrumental texture analyzer. It was concluded that for healthy juvenile salmon reared under controlled growth conditions, the dietary vitamin C inclusion of 79 mg kg(-1) of wet mass feed was sufficient to produce the required synthesis of soluble muscle collagen. Furthermore, post-translational modifications of the collagen leading to cross-linking showed a small decrease with increasing growth rate but was independent of vitamin C concentration in the diet at these levels. |
format |
Article in Journal/Newspaper |
author |
Li, X J Bickerdike, R Nickell, D Campbell, P Dingwall, A Johnston, Ian Alistair |
author_facet |
Li, X J Bickerdike, R Nickell, D Campbell, P Dingwall, A Johnston, Ian Alistair |
author_sort |
Li, X J |
title |
Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). |
title_short |
Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). |
title_full |
Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). |
title_fullStr |
Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). |
title_full_unstemmed |
Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). |
title_sort |
investigations on the effects of growth rate and dietary vitamin c on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed atlantic salmon ( salmo salar ). |
publishDate |
2007 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/investigations-on-the-effects-of-growth-rate-and-dietary-vitamin-c-on-skeletal-muscle-collagen-and-hydroxylysyl-pyridinoline-crosslink-concentration-in-farmed-atlantic-salmon-salmo-salar(210d55a0-378b-4a94-9f4c-408a4b1c81e6).html https://doi.org/10.1021/jf062251i http://www.scopus.com/inward/record.url?scp=34047229537&partnerID=8YFLogxK |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Li , X J , Bickerdike , R , Nickell , D , Campbell , P , Dingwall , A & Johnston , I A 2007 , ' Investigations on the effects of growth rate and dietary vitamin C on skeletal muscle collagen and hydroxylysyl pyridinoline cross-link concentration in farmed Atlantic salmon ( Salmo salar ). ' , Journal of Agricultural and Food Chemistry , vol. 55 , no. 2 , pp. 510-515 . https://doi.org/10.1021/jf062251i |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1021/jf062251i |
container_title |
Journal of Agricultural and Food Chemistry |
container_volume |
55 |
container_issue |
2 |
container_start_page |
510 |
op_container_end_page |
515 |
_version_ |
1766362149379112960 |