Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals
An animal-borne blood sampler with data-logging functions was developed for phocid seals, which collected two blood samples for the comparison of endocrino-logical/biochemical parameters under two different conditions. The sampler can be triggered by preset hydrostatic pressure, acceleration (descen...
Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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2016
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Online Access: | https://risweb.st-andrews.ac.uk/portal/en/researchoutput/development-of-an-animalborne-blood-sample-collection-device-and-its-deployment-for-the-determination-of-cardiovascular-and-stress-hormones-in-phocid-seals(0339f705-f833-47da-ab69-c2f4bd22e525).html https://doi.org/10.1152/ajpregu.00211.2016 https://research-repository.st-andrews.ac.uk/bitstream/10023/11456/1/AJP_R_00211_2016_Accepted.pdf |
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ftunstandrewcris:oai:risweb.st-andrews.ac.uk:publications/0339f705-f833-47da-ab69-c2f4bd22e525 2023-05-15T17:58:57+02:00 Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals Takei, Yoshio Suzuki, Ippei Wong, Marty K. S. Milne, Ryan Moss, Simon Sato, Katsufumi Hall, Ailsa 2016-10-06 application/pdf https://risweb.st-andrews.ac.uk/portal/en/researchoutput/development-of-an-animalborne-blood-sample-collection-device-and-its-deployment-for-the-determination-of-cardiovascular-and-stress-hormones-in-phocid-seals(0339f705-f833-47da-ab69-c2f4bd22e525).html https://doi.org/10.1152/ajpregu.00211.2016 https://research-repository.st-andrews.ac.uk/bitstream/10023/11456/1/AJP_R_00211_2016_Accepted.pdf eng eng info:eu-repo/semantics/openAccess Takei , Y , Suzuki , I , Wong , M K S , Milne , R , Moss , S , Sato , K & Hall , A 2016 , ' Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 311 , no. 4 , pp. R788-R796 . https://doi.org/10.1152/ajpregu.00211.2016 Cardiovascular hormone Data logger Gravity Sea mammal Stress hormone article 2016 ftunstandrewcris https://doi.org/10.1152/ajpregu.00211.2016 2022-06-02T07:46:43Z An animal-borne blood sampler with data-logging functions was developed for phocid seals, which collected two blood samples for the comparison of endocrino-logical/biochemical parameters under two different conditions. The sampler can be triggered by preset hydrostatic pressure, acceleration (descending or ascending), temperature, and time, and also man-ually by light. The sampling was reliable with 39/50 (78%) successful attempts to collect blood samples. Contamination of fluids in the tubing to the next blood sample was 1%, following the prior clearance of the tubing to a waste syringe. In captive harbor seals ( Phoca vitulina ), the automated blood-sampling method was less stressful than direct blood withdrawal, as evidenced by lower levels of stress hormones ( P < 0.05 for ACTH and P = 0.078 for cortisol). HPLC analyses showed that both cortisol and cortisone were circu-lating in seal blood. Using the sampler, plasma levels of cardiovascular hormones, atrial natriuretic peptide (ANP), AVP, and ANG II were compared in grey seals ( Halichoerus grypus ), between samples collected when the animals were on land and in the water. HPLC analyses determined that [Met 12 ] ANP (1-28) and various forms of angiotensins (ANG II, III, and IV) were circulating in seal blood. Although water immersion profoundly changes the plasma levels of cardiovascular hormones in terrestrial mammals, there were only tendencies toward an increase in ANP ( P = 0.069) and a decrease in AVP ( P = 0.074) in the seals. These results suggest that cardiovascular regulation in phocid seals may have undergone adaptation during evolution of the carnivore to a semiaquatic lifestyle. Article in Journal/Newspaper Phoca vitulina University of St Andrews: Research Portal American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 311 4 R788 R796 |
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Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
Cardiovascular hormone Data logger Gravity Sea mammal Stress hormone |
spellingShingle |
Cardiovascular hormone Data logger Gravity Sea mammal Stress hormone Takei, Yoshio Suzuki, Ippei Wong, Marty K. S. Milne, Ryan Moss, Simon Sato, Katsufumi Hall, Ailsa Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
topic_facet |
Cardiovascular hormone Data logger Gravity Sea mammal Stress hormone |
description |
An animal-borne blood sampler with data-logging functions was developed for phocid seals, which collected two blood samples for the comparison of endocrino-logical/biochemical parameters under two different conditions. The sampler can be triggered by preset hydrostatic pressure, acceleration (descending or ascending), temperature, and time, and also man-ually by light. The sampling was reliable with 39/50 (78%) successful attempts to collect blood samples. Contamination of fluids in the tubing to the next blood sample was 1%, following the prior clearance of the tubing to a waste syringe. In captive harbor seals ( Phoca vitulina ), the automated blood-sampling method was less stressful than direct blood withdrawal, as evidenced by lower levels of stress hormones ( P < 0.05 for ACTH and P = 0.078 for cortisol). HPLC analyses showed that both cortisol and cortisone were circu-lating in seal blood. Using the sampler, plasma levels of cardiovascular hormones, atrial natriuretic peptide (ANP), AVP, and ANG II were compared in grey seals ( Halichoerus grypus ), between samples collected when the animals were on land and in the water. HPLC analyses determined that [Met 12 ] ANP (1-28) and various forms of angiotensins (ANG II, III, and IV) were circulating in seal blood. Although water immersion profoundly changes the plasma levels of cardiovascular hormones in terrestrial mammals, there were only tendencies toward an increase in ANP ( P = 0.069) and a decrease in AVP ( P = 0.074) in the seals. These results suggest that cardiovascular regulation in phocid seals may have undergone adaptation during evolution of the carnivore to a semiaquatic lifestyle. |
format |
Article in Journal/Newspaper |
author |
Takei, Yoshio Suzuki, Ippei Wong, Marty K. S. Milne, Ryan Moss, Simon Sato, Katsufumi Hall, Ailsa |
author_facet |
Takei, Yoshio Suzuki, Ippei Wong, Marty K. S. Milne, Ryan Moss, Simon Sato, Katsufumi Hall, Ailsa |
author_sort |
Takei, Yoshio |
title |
Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
title_short |
Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
title_full |
Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
title_fullStr |
Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
title_full_unstemmed |
Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
title_sort |
development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals |
publishDate |
2016 |
url |
https://risweb.st-andrews.ac.uk/portal/en/researchoutput/development-of-an-animalborne-blood-sample-collection-device-and-its-deployment-for-the-determination-of-cardiovascular-and-stress-hormones-in-phocid-seals(0339f705-f833-47da-ab69-c2f4bd22e525).html https://doi.org/10.1152/ajpregu.00211.2016 https://research-repository.st-andrews.ac.uk/bitstream/10023/11456/1/AJP_R_00211_2016_Accepted.pdf |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Takei , Y , Suzuki , I , Wong , M K S , Milne , R , Moss , S , Sato , K & Hall , A 2016 , ' Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 311 , no. 4 , pp. R788-R796 . https://doi.org/10.1152/ajpregu.00211.2016 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1152/ajpregu.00211.2016 |
container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
container_volume |
311 |
container_issue |
4 |
container_start_page |
R788 |
op_container_end_page |
R796 |
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1766167685456986112 |