A radioimmunoassay for the determination of angiotensin II in elasmobranch fish
A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were...
Published in: | General and Comparative Endocrinology |
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Language: | English |
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1998
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/a-radioimmunoassay-for-the-determination-of-angiotensin-ii-in-elasmobranch-fish(ff516434-89a9-4d02-a1bd-bd4621941aaf).html https://doi.org/10.1006/gcen.1998.7114 http://www.scopus.com/inward/record.url?scp=0031875306&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/ff516434-89a9-4d02-a1bd-bd4621941aaf 2024-06-23T07:45:33+00:00 A radioimmunoassay for the determination of angiotensin II in elasmobranch fish Tierney, ML Takei, Y Hazon, Neil 1998-09 https://research-portal.st-andrews.ac.uk/en/researchoutput/a-radioimmunoassay-for-the-determination-of-angiotensin-ii-in-elasmobranch-fish(ff516434-89a9-4d02-a1bd-bd4621941aaf).html https://doi.org/10.1006/gcen.1998.7114 http://www.scopus.com/inward/record.url?scp=0031875306&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/a-radioimmunoassay-for-the-determination-of-angiotensin-ii-in-elasmobranch-fish(ff516434-89a9-4d02-a1bd-bd4621941aaf).html info:eu-repo/semantics/restrictedAccess Tierney , ML , Takei , Y & Hazon , N 1998 , ' A radioimmunoassay for the determination of angiotensin II in elasmobranch fish ' , General and Comparative Endocrinology , vol. 111 , no. 3 , pp. 299-305 . https://doi.org/10.1006/gcen.1998.7114 ANGUILLA-ANGUILLA SYSTEM PLASMA EEL article 1998 ftunstandrewcris https://doi.org/10.1006/gcen.1998.7114 2024-06-13T00:03:34Z A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press. Article in Journal/Newspaper Anguilla anguilla University of St Andrews: Research Portal General and Comparative Endocrinology 111 3 299 305 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
ANGUILLA-ANGUILLA SYSTEM PLASMA EEL |
spellingShingle |
ANGUILLA-ANGUILLA SYSTEM PLASMA EEL Tierney, ML Takei, Y Hazon, Neil A radioimmunoassay for the determination of angiotensin II in elasmobranch fish |
topic_facet |
ANGUILLA-ANGUILLA SYSTEM PLASMA EEL |
description |
A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press. |
format |
Article in Journal/Newspaper |
author |
Tierney, ML Takei, Y Hazon, Neil |
author_facet |
Tierney, ML Takei, Y Hazon, Neil |
author_sort |
Tierney, ML |
title |
A radioimmunoassay for the determination of angiotensin II in elasmobranch fish |
title_short |
A radioimmunoassay for the determination of angiotensin II in elasmobranch fish |
title_full |
A radioimmunoassay for the determination of angiotensin II in elasmobranch fish |
title_fullStr |
A radioimmunoassay for the determination of angiotensin II in elasmobranch fish |
title_full_unstemmed |
A radioimmunoassay for the determination of angiotensin II in elasmobranch fish |
title_sort |
radioimmunoassay for the determination of angiotensin ii in elasmobranch fish |
publishDate |
1998 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/a-radioimmunoassay-for-the-determination-of-angiotensin-ii-in-elasmobranch-fish(ff516434-89a9-4d02-a1bd-bd4621941aaf).html https://doi.org/10.1006/gcen.1998.7114 http://www.scopus.com/inward/record.url?scp=0031875306&partnerID=8YFLogxK |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Tierney , ML , Takei , Y & Hazon , N 1998 , ' A radioimmunoassay for the determination of angiotensin II in elasmobranch fish ' , General and Comparative Endocrinology , vol. 111 , no. 3 , pp. 299-305 . https://doi.org/10.1006/gcen.1998.7114 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/a-radioimmunoassay-for-the-determination-of-angiotensin-ii-in-elasmobranch-fish(ff516434-89a9-4d02-a1bd-bd4621941aaf).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1006/gcen.1998.7114 |
container_title |
General and Comparative Endocrinology |
container_volume |
111 |
container_issue |
3 |
container_start_page |
299 |
op_container_end_page |
305 |
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1802640735748489216 |