A radioimmunoassay for the determination of angiotensin II in elasmobranch fish

• Published in: General and Comparative Endocrinology
• Main Authors: Tierney, ML, Takei, Y, Hazon, Neil
• Format: Article in Journal/Newspaper
• Language: English
• Published: 1998
• Subjects: ANGUILLA-ANGUILLA; SYSTEM; PLASMA; EEL; Anguilla anguilla
• Online Access: https://research-portal.st-andrews.ac.uk/en/researchoutput/a-radioimmunoassay-for-the-determination-of-angiotensin-ii-in-elasmobranch-fish(ff516434-89a9-4d02-a1bd-bd4621941aaf).html; https://doi.org/10.1006/gcen.1998.7114; http://www.scopus.com/inward/record.url?scp=0031875306&partnerID=8YFLogxK

A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press.