Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L).
Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effects of st bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated...
Published in: | Fish & Shellfish Immunology |
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Format: | Other/Unknown Material |
Language: | English |
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2001
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/response-of-haemocyte-lysosomes-to-bacterial-inoculation-in-the-oysters-ostrea-edulis-l-and-crassostrea-gigas-thunberg-and-the-scallop-pecten-maximus-l(fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2).html https://doi.org/10.1006/fsim.2000.0301 |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2 2024-06-23T07:52:15+00:00 Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). Hauton, Christopher Hawkins, LE Hutchinson, S 2001-02 https://research-portal.st-andrews.ac.uk/en/researchoutput/response-of-haemocyte-lysosomes-to-bacterial-inoculation-in-the-oysters-ostrea-edulis-l-and-crassostrea-gigas-thunberg-and-the-scallop-pecten-maximus-l(fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2).html https://doi.org/10.1006/fsim.2000.0301 eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/response-of-haemocyte-lysosomes-to-bacterial-inoculation-in-the-oysters-ostrea-edulis-l-and-crassostrea-gigas-thunberg-and-the-scallop-pecten-maximus-l(fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2).html info:eu-repo/semantics/restrictedAccess Hauton , C , Hawkins , LE & Hutchinson , S 2001 , Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). . https://doi.org/10.1006/fsim.2000.0301 neutral red retention assay lysosomes haemocytes Crassostrea gigas Ostrea edulis Pecten maximus Listonella anguillarum immune status MEMBRANE DAMAGE DIGESTIVE CELLS IN-VITRO MUSSELS ASSAY TEMPERATURE LISTONELLA HEMOCYTES SALINITY BLOOD other 2001 ftunstandrewcris https://doi.org/10.1006/fsim.2000.0301 2024-06-13T00:14:39Z Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effects of st bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to three temperature regimes (5, 15 and 25 degrees C), at constant salinity for 7 days in the laboratory. Once baseline responses to acclimation temperature had been established, the effects of an in vivo inoculation on haemocyte lysosomal stability were assessed using th NRR assay. Lysosomal membrane stability was reduced in the presence of bacteria for all three species of bivalve, but destabilisation of C. gigas haemocyte lysosomes appeared to be most sensitive to the presence of the bacterium Listonella anguillarum. For all three bivalve species, the reduction in lysosomal stability appeared to be proportional to the growth of the bacterial inoculate. Using appropriate controls, the NRR assay was demonstrated to have great potential as a tool with which to make rapid initial assessments of the immune status of bivalve molluscs. (C) 2001 Academic Press. Other/Unknown Material Crassostrea gigas Pacific oyster University of St Andrews: Research Portal Pacific Fish & Shellfish Immunology 11 2 143 153 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
neutral red retention assay lysosomes haemocytes Crassostrea gigas Ostrea edulis Pecten maximus Listonella anguillarum immune status MEMBRANE DAMAGE DIGESTIVE CELLS IN-VITRO MUSSELS ASSAY TEMPERATURE LISTONELLA HEMOCYTES SALINITY BLOOD |
spellingShingle |
neutral red retention assay lysosomes haemocytes Crassostrea gigas Ostrea edulis Pecten maximus Listonella anguillarum immune status MEMBRANE DAMAGE DIGESTIVE CELLS IN-VITRO MUSSELS ASSAY TEMPERATURE LISTONELLA HEMOCYTES SALINITY BLOOD Hauton, Christopher Hawkins, LE Hutchinson, S Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
topic_facet |
neutral red retention assay lysosomes haemocytes Crassostrea gigas Ostrea edulis Pecten maximus Listonella anguillarum immune status MEMBRANE DAMAGE DIGESTIVE CELLS IN-VITRO MUSSELS ASSAY TEMPERATURE LISTONELLA HEMOCYTES SALINITY BLOOD |
description |
Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effects of st bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to three temperature regimes (5, 15 and 25 degrees C), at constant salinity for 7 days in the laboratory. Once baseline responses to acclimation temperature had been established, the effects of an in vivo inoculation on haemocyte lysosomal stability were assessed using th NRR assay. Lysosomal membrane stability was reduced in the presence of bacteria for all three species of bivalve, but destabilisation of C. gigas haemocyte lysosomes appeared to be most sensitive to the presence of the bacterium Listonella anguillarum. For all three bivalve species, the reduction in lysosomal stability appeared to be proportional to the growth of the bacterial inoculate. Using appropriate controls, the NRR assay was demonstrated to have great potential as a tool with which to make rapid initial assessments of the immune status of bivalve molluscs. (C) 2001 Academic Press. |
format |
Other/Unknown Material |
author |
Hauton, Christopher Hawkins, LE Hutchinson, S |
author_facet |
Hauton, Christopher Hawkins, LE Hutchinson, S |
author_sort |
Hauton, Christopher |
title |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_short |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_full |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_fullStr |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_full_unstemmed |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_sort |
response of haemocyte lysosomes to bacterial inoculation in the oysters ostrea edulis l. and crassostrea gigas (thunberg), and the scallop pecten maximus (l). |
publishDate |
2001 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/response-of-haemocyte-lysosomes-to-bacterial-inoculation-in-the-oysters-ostrea-edulis-l-and-crassostrea-gigas-thunberg-and-the-scallop-pecten-maximus-l(fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2).html https://doi.org/10.1006/fsim.2000.0301 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hauton , C , Hawkins , LE & Hutchinson , S 2001 , Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). . https://doi.org/10.1006/fsim.2000.0301 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/response-of-haemocyte-lysosomes-to-bacterial-inoculation-in-the-oysters-ostrea-edulis-l-and-crassostrea-gigas-thunberg-and-the-scallop-pecten-maximus-l(fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1006/fsim.2000.0301 |
container_title |
Fish & Shellfish Immunology |
container_volume |
11 |
container_issue |
2 |
container_start_page |
143 |
op_container_end_page |
153 |
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1802643502034583552 |