Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L).

Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effects of st bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Hauton, Christopher, Hawkins, LE, Hutchinson, S
Format: Other/Unknown Material
Language:English
Published: 2001
Subjects:
neutral red retention assay
lysosomes
haemocytes
Crassostrea gigas
Ostrea edulis
Pecten maximus
Listonella anguillarum
immune status
MEMBRANE DAMAGE
DIGESTIVE CELLS
IN-VITRO
MUSSELS
ASSAY
TEMPERATURE
LISTONELLA
HEMOCYTES
SALINITY
BLOOD
Pacific
Pacific oyster
Online Access:https://research-portal.st-andrews.ac.uk/en/researchoutput/response-of-haemocyte-lysosomes-to-bacterial-inoculation-in-the-oysters-ostrea-edulis-l-and-crassostrea-gigas-thunberg-and-the-scallop-pecten-maximus-l(fc5a8436-b888-4bcb-abe6-3dfd8c39a6e2).html
https://doi.org/10.1006/fsim.2000.0301
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Summary:Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effects of st bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to three temperature regimes (5, 15 and 25 degrees C), at constant salinity for 7 days in the laboratory. Once baseline responses to acclimation temperature had been established, the effects of an in vivo inoculation on haemocyte lysosomal stability were assessed using th NRR assay. Lysosomal membrane stability was reduced in the presence of bacteria for all three species of bivalve, but destabilisation of C. gigas haemocyte lysosomes appeared to be most sensitive to the presence of the bacterium Listonella anguillarum. For all three bivalve species, the reduction in lysosomal stability appeared to be proportional to the growth of the bacterial inoculate. Using appropriate controls, the NRR assay was demonstrated to have great potential as a tool with which to make rapid initial assessments of the immune status of bivalve molluscs. (C) 2001 Academic Press.

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