Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds
Real-time PCR was used to measure changes in transcript abundance of genes encoding important immune proteins, namely prophenoloxidase (proPO gene), beta-1,3-glucan binding protein (beta GBP gene) and a 12.2 kDa antimicrobial peptide (amp gene) in post-larval stage VI (PLVI) juveniles of the Europea...
Published in: | Molecular Immunology |
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2007
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/changes-in-immune-gene-expression-and-resistance-to-bacterial-infection-in-lobster-homarus-gammarus-postlarval-stage-vi-following-acute-or-chronic-exposure-to-immune-stimulating-compounds(a534b963-3733-4c9f-ad03-96f04f687895).html https://doi.org/10.1016/j.molimm.2006.02.018 http://www.scopus.com/inward/record.url?scp=33747869917&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/a534b963-3733-4c9f-ad03-96f04f687895 2024-06-23T07:52:35+00:00 Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds Hauton, C. Brockton, V. Smith, V. J. 2007-01 https://research-portal.st-andrews.ac.uk/en/researchoutput/changes-in-immune-gene-expression-and-resistance-to-bacterial-infection-in-lobster-homarus-gammarus-postlarval-stage-vi-following-acute-or-chronic-exposure-to-immune-stimulating-compounds(a534b963-3733-4c9f-ad03-96f04f687895).html https://doi.org/10.1016/j.molimm.2006.02.018 http://www.scopus.com/inward/record.url?scp=33747869917&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/changes-in-immune-gene-expression-and-resistance-to-bacterial-infection-in-lobster-homarus-gammarus-postlarval-stage-vi-following-acute-or-chronic-exposure-to-immune-stimulating-compounds(a534b963-3733-4c9f-ad03-96f04f687895).html info:eu-repo/semantics/restrictedAccess Hauton , C , Brockton , V & Smith , V J 2007 , ' Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds ' , Molecular Immunology , vol. 44 , pp. 443-450 . https://doi.org/10.1016/j.molimm.2006.02.018 disease resistance gene expression Homarus gammarus immunostimutant lobster real-time PCR SHRIMP PENAEUS-MONODON PROPHENOLOXIDASE-ACTIVATING SYSTEM BLACK TIGER SHRIMP MESSENGER-RNA MACROBRACHIUM-ROSENBERGII MARSUPENAEUS-JAPONICUS ANTIBACTERIAL PEPTIDE INVERTEBRATE IMMUNITY LITOPENAEUS-VANNAMEI article 2007 ftunstandrewcris https://doi.org/10.1016/j.molimm.2006.02.018 2024-06-13T00:24:00Z Real-time PCR was used to measure changes in transcript abundance of genes encoding important immune proteins, namely prophenoloxidase (proPO gene), beta-1,3-glucan binding protein (beta GBP gene) and a 12.2 kDa antimicrobial peptide (amp gene) in post-larval stage VI (PLVI) juveniles of the European lobster, Homarus gammarus. Gene expression was studied in both healthy PLVI and following single or repeat exposure to a range of compounds claimed to induce immune reactivity. A single acute (3-h) exposure to any of the tested stimulants did not produce a significant increase in expression of either the proPO or beta GBP genes, measured 6 h after stimulation. However, there were a small sub-group of positive responders. identified mainly from beta GBP expression, within the experimental groups stimulated with either a beta-1,3-glucan or an alginate. There was also no significant increase in the expression of any of the three genes tested 24 h after repeated weekly (3-h) exposures to a either the beta-1,3-alucan or the alginate over the lonaer (36-day) period. The results do show that amp is expressed at an extremely high level compared to proPO or beta GBP in healthy animals and a significant correlation was found between the expression of proPO and both beta GBP and amp, irrespective of whether or not the larvae were stimulated. None of the immune stimulated compounds improved survival of PLVI challenged with the opportunistic pathogen, Listonella anguillarum, or the lobster pathogen, Aerococcus viridans var. homari. Thus, we found no evidence to support recent claims that immunity and disease resistance can be primed or promoted within a given population of crustaceans or that these animals exhibit functional immune memory to some soluble immune elicitors. (c) 2006 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper European lobster Homarus gammarus University of St Andrews: Research Portal Molecular Immunology 44 4 443 450 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
disease resistance gene expression Homarus gammarus immunostimutant lobster real-time PCR SHRIMP PENAEUS-MONODON PROPHENOLOXIDASE-ACTIVATING SYSTEM BLACK TIGER SHRIMP MESSENGER-RNA MACROBRACHIUM-ROSENBERGII MARSUPENAEUS-JAPONICUS ANTIBACTERIAL PEPTIDE INVERTEBRATE IMMUNITY LITOPENAEUS-VANNAMEI |
spellingShingle |
disease resistance gene expression Homarus gammarus immunostimutant lobster real-time PCR SHRIMP PENAEUS-MONODON PROPHENOLOXIDASE-ACTIVATING SYSTEM BLACK TIGER SHRIMP MESSENGER-RNA MACROBRACHIUM-ROSENBERGII MARSUPENAEUS-JAPONICUS ANTIBACTERIAL PEPTIDE INVERTEBRATE IMMUNITY LITOPENAEUS-VANNAMEI Hauton, C. Brockton, V. Smith, V. J. Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds |
topic_facet |
disease resistance gene expression Homarus gammarus immunostimutant lobster real-time PCR SHRIMP PENAEUS-MONODON PROPHENOLOXIDASE-ACTIVATING SYSTEM BLACK TIGER SHRIMP MESSENGER-RNA MACROBRACHIUM-ROSENBERGII MARSUPENAEUS-JAPONICUS ANTIBACTERIAL PEPTIDE INVERTEBRATE IMMUNITY LITOPENAEUS-VANNAMEI |
description |
Real-time PCR was used to measure changes in transcript abundance of genes encoding important immune proteins, namely prophenoloxidase (proPO gene), beta-1,3-glucan binding protein (beta GBP gene) and a 12.2 kDa antimicrobial peptide (amp gene) in post-larval stage VI (PLVI) juveniles of the European lobster, Homarus gammarus. Gene expression was studied in both healthy PLVI and following single or repeat exposure to a range of compounds claimed to induce immune reactivity. A single acute (3-h) exposure to any of the tested stimulants did not produce a significant increase in expression of either the proPO or beta GBP genes, measured 6 h after stimulation. However, there were a small sub-group of positive responders. identified mainly from beta GBP expression, within the experimental groups stimulated with either a beta-1,3-glucan or an alginate. There was also no significant increase in the expression of any of the three genes tested 24 h after repeated weekly (3-h) exposures to a either the beta-1,3-alucan or the alginate over the lonaer (36-day) period. The results do show that amp is expressed at an extremely high level compared to proPO or beta GBP in healthy animals and a significant correlation was found between the expression of proPO and both beta GBP and amp, irrespective of whether or not the larvae were stimulated. None of the immune stimulated compounds improved survival of PLVI challenged with the opportunistic pathogen, Listonella anguillarum, or the lobster pathogen, Aerococcus viridans var. homari. Thus, we found no evidence to support recent claims that immunity and disease resistance can be primed or promoted within a given population of crustaceans or that these animals exhibit functional immune memory to some soluble immune elicitors. (c) 2006 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Hauton, C. Brockton, V. Smith, V. J. |
author_facet |
Hauton, C. Brockton, V. Smith, V. J. |
author_sort |
Hauton, C. |
title |
Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds |
title_short |
Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds |
title_full |
Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds |
title_fullStr |
Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds |
title_full_unstemmed |
Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds |
title_sort |
changes in immune gene expression and resistance to bacterial infection in lobster (homarus gammarus) post-larval stage vi following acute or chronic exposure to immune stimulating compounds |
publishDate |
2007 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/changes-in-immune-gene-expression-and-resistance-to-bacterial-infection-in-lobster-homarus-gammarus-postlarval-stage-vi-following-acute-or-chronic-exposure-to-immune-stimulating-compounds(a534b963-3733-4c9f-ad03-96f04f687895).html https://doi.org/10.1016/j.molimm.2006.02.018 http://www.scopus.com/inward/record.url?scp=33747869917&partnerID=8YFLogxK |
genre |
European lobster Homarus gammarus |
genre_facet |
European lobster Homarus gammarus |
op_source |
Hauton , C , Brockton , V & Smith , V J 2007 , ' Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compounds ' , Molecular Immunology , vol. 44 , pp. 443-450 . https://doi.org/10.1016/j.molimm.2006.02.018 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/changes-in-immune-gene-expression-and-resistance-to-bacterial-infection-in-lobster-homarus-gammarus-postlarval-stage-vi-following-acute-or-chronic-exposure-to-immune-stimulating-compounds(a534b963-3733-4c9f-ad03-96f04f687895).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.molimm.2006.02.018 |
container_title |
Molecular Immunology |
container_volume |
44 |
container_issue |
4 |
container_start_page |
443 |
op_container_end_page |
450 |
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1802643932860907520 |