Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla)
Invasive parasites have been implicated in the declines of several freshwater species. The swim bladder nematode Anguillicola crassus was introduced into Europe in the 1980s and is considered a threat to the European eel (Anguilla anguilla). Infection affects stress resistance and swimming behaviour...
Published in: | Marine Genomics |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2019
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/experimental-infection-with-anguillicola-crassus-alters-immune-gene-expression-in-both-spleen-and-head-kidney-of-the-european-eel-anguilla-anguilla(a07ce6fe-eba8-4a11-99c4-f799172a0632).html https://doi.org/10.1016/j.margen.2018.12.002 |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/a07ce6fe-eba8-4a11-99c4-f799172a0632 2024-05-12T07:52:57+00:00 Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) Bracamonte, Seraina E Johnston, Paul R Knopf, Klaus Monaghan, Michael T 2019-06 https://research-portal.st-andrews.ac.uk/en/researchoutput/experimental-infection-with-anguillicola-crassus-alters-immune-gene-expression-in-both-spleen-and-head-kidney-of-the-european-eel-anguilla-anguilla(a07ce6fe-eba8-4a11-99c4-f799172a0632).html https://doi.org/10.1016/j.margen.2018.12.002 eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/experimental-infection-with-anguillicola-crassus-alters-immune-gene-expression-in-both-spleen-and-head-kidney-of-the-european-eel-anguilla-anguilla(a07ce6fe-eba8-4a11-99c4-f799172a0632).html info:eu-repo/semantics/restrictedAccess Bracamonte , S E , Johnston , P R , Knopf , K & Monaghan , M T 2019 , ' Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) ' , Marine genomics , vol. 45 , pp. 28-37 . https://doi.org/10.1016/j.margen.2018.12.002 Anguilla Animals Dracunculoidea/physiology Fish Diseases/genetics Gene Expression/immunology Head Kidney/immunology Spirurida Infections/genetics Spleen/immunology article 2019 ftunstandrewcris https://doi.org/10.1016/j.margen.2018.12.002 2024-04-16T02:39:21Z Invasive parasites have been implicated in the declines of several freshwater species. The swim bladder nematode Anguillicola crassus was introduced into Europe in the 1980s and is considered a threat to the European eel (Anguilla anguilla). Infection affects stress resistance and swimming behaviour. European eels produce an immune response against the parasite during the late stages of infection and after repeated infections. We used RNA-seq to examine the molecular response to infection during the poorly understood early stage and identify expression of genes and associated processes that are modified in two immune organs of European eels 3 days post infection with A. crassus. In the spleen, 67 genes were differentially expressed, 32 of which were annotated. Most of these were involved in immune processes and their regulation. Other differentially expressed genes in the spleen were important for heme metabolism and heme turn-over. In the head kidney, 257 genes (134 annotated) were differentially expressed. Several of these were associated with immune functions. Other differentially expressed genes in the head kidney were related to renal function, in particular osmoregulation and paracellular flow. We conclude that the early response of European eels to A. crassus is complex and involves various processes aside from the immune system. We identified molecular changes occurring early during the infection and identified candidate genes and processes which will facilitate future studies aimed at determining the factors affecting European eel viability in the face of this invasive parasite. Article in Journal/Newspaper Anguilla anguilla European eel University of St Andrews: Research Portal Marine Genomics 45 28 37 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
Anguilla Animals Dracunculoidea/physiology Fish Diseases/genetics Gene Expression/immunology Head Kidney/immunology Spirurida Infections/genetics Spleen/immunology |
spellingShingle |
Anguilla Animals Dracunculoidea/physiology Fish Diseases/genetics Gene Expression/immunology Head Kidney/immunology Spirurida Infections/genetics Spleen/immunology Bracamonte, Seraina E Johnston, Paul R Knopf, Klaus Monaghan, Michael T Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) |
topic_facet |
Anguilla Animals Dracunculoidea/physiology Fish Diseases/genetics Gene Expression/immunology Head Kidney/immunology Spirurida Infections/genetics Spleen/immunology |
description |
Invasive parasites have been implicated in the declines of several freshwater species. The swim bladder nematode Anguillicola crassus was introduced into Europe in the 1980s and is considered a threat to the European eel (Anguilla anguilla). Infection affects stress resistance and swimming behaviour. European eels produce an immune response against the parasite during the late stages of infection and after repeated infections. We used RNA-seq to examine the molecular response to infection during the poorly understood early stage and identify expression of genes and associated processes that are modified in two immune organs of European eels 3 days post infection with A. crassus. In the spleen, 67 genes were differentially expressed, 32 of which were annotated. Most of these were involved in immune processes and their regulation. Other differentially expressed genes in the spleen were important for heme metabolism and heme turn-over. In the head kidney, 257 genes (134 annotated) were differentially expressed. Several of these were associated with immune functions. Other differentially expressed genes in the head kidney were related to renal function, in particular osmoregulation and paracellular flow. We conclude that the early response of European eels to A. crassus is complex and involves various processes aside from the immune system. We identified molecular changes occurring early during the infection and identified candidate genes and processes which will facilitate future studies aimed at determining the factors affecting European eel viability in the face of this invasive parasite. |
format |
Article in Journal/Newspaper |
author |
Bracamonte, Seraina E Johnston, Paul R Knopf, Klaus Monaghan, Michael T |
author_facet |
Bracamonte, Seraina E Johnston, Paul R Knopf, Klaus Monaghan, Michael T |
author_sort |
Bracamonte, Seraina E |
title |
Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) |
title_short |
Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) |
title_full |
Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) |
title_fullStr |
Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) |
title_full_unstemmed |
Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) |
title_sort |
experimental infection with anguillicola crassus alters immune gene expression in both spleen and head kidney of the european eel (anguilla anguilla) |
publishDate |
2019 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/experimental-infection-with-anguillicola-crassus-alters-immune-gene-expression-in-both-spleen-and-head-kidney-of-the-european-eel-anguilla-anguilla(a07ce6fe-eba8-4a11-99c4-f799172a0632).html https://doi.org/10.1016/j.margen.2018.12.002 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Bracamonte , S E , Johnston , P R , Knopf , K & Monaghan , M T 2019 , ' Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla) ' , Marine genomics , vol. 45 , pp. 28-37 . https://doi.org/10.1016/j.margen.2018.12.002 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/experimental-infection-with-anguillicola-crassus-alters-immune-gene-expression-in-both-spleen-and-head-kidney-of-the-european-eel-anguilla-anguilla(a07ce6fe-eba8-4a11-99c4-f799172a0632).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.margen.2018.12.002 |
container_title |
Marine Genomics |
container_volume |
45 |
container_start_page |
28 |
op_container_end_page |
37 |
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1798839211969740800 |