DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR
This work demonstrates the feasibility of making sensitive nanometer distancemeasurements between Fe(III) heme centers and nitroxide spin labels in proteins using the double electron-electron resonance (DEER) pulsed EPR technique at 94 GHz. Techniques to measure accurately long distances in many cla...
Published in: | The Journal of Physical Chemistry Letters |
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2016
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/deer-sensitivity-between-iron-centers-and-nitroxides-in-hemecontaining-proteins-improves-dramatically-using-broadband-highfield-epr(9edcefd2-4287-4822-a219-d87594cc1154).html https://doi.org/10.1021/acs.jpclett.6b00456 https://research-repository.st-andrews.ac.uk/bitstream/10023/8779/1/Motion_2016_DEERsensitivity_JPCL_CC.pdf |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/9edcefd2-4287-4822-a219-d87594cc1154 2024-06-23T07:56:58+00:00 DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR Motion, Claire Louise Lovett, Janet Eleanor Bell, Stacey Cassidy, Scott Lindsay Cruickshank, Paul Alexander Sawchuk Bolton, David Robert Hunter, Robert Iain El Mkami, Hassane Van Doorslaer, Sabine Smith, Graham Murray 2016-04-21 application/pdf https://research-portal.st-andrews.ac.uk/en/researchoutput/deer-sensitivity-between-iron-centers-and-nitroxides-in-hemecontaining-proteins-improves-dramatically-using-broadband-highfield-epr(9edcefd2-4287-4822-a219-d87594cc1154).html https://doi.org/10.1021/acs.jpclett.6b00456 https://research-repository.st-andrews.ac.uk/bitstream/10023/8779/1/Motion_2016_DEERsensitivity_JPCL_CC.pdf eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/deer-sensitivity-between-iron-centers-and-nitroxides-in-hemecontaining-proteins-improves-dramatically-using-broadband-highfield-epr(9edcefd2-4287-4822-a219-d87594cc1154).html info:eu-repo/semantics/openAccess Motion , C L , Lovett , J E , Bell , S , Cassidy , S L , Cruickshank , P A S , Bolton , D R , Hunter , R I , El Mkami , H , Van Doorslaer , S & Smith , G M 2016 , ' DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR ' , Journal of Physical Chemistry Letters , vol. 7 , no. 8 , pp. 1411-1415 . https://doi.org/10.1021/acs.jpclett.6b00456 article 2016 ftunstandrewcris https://doi.org/10.1021/acs.jpclett.6b00456 2024-06-13T00:51:34Z This work demonstrates the feasibility of making sensitive nanometer distancemeasurements between Fe(III) heme centers and nitroxide spin labels in proteins using the double electron-electron resonance (DEER) pulsed EPR technique at 94 GHz. Techniques to measure accurately long distances in many classes of heme proteins using DEER are currently strongly limited by sensitivity. In this paper we demonstrate sensitivity gains of more than 30times compared with previous lower frequency (X-band) DEER measurements on both human neuroglobin and sperm whale myoglobin. This is achieved by: taking advantage of recent instrumental advances; employing wideband excitation techniques based on composite pulses and exploiting more favorable relaxation properties of low-spin Fe(III) in high magnetic fields. This gain in sensitivity potentially allows the DEER technique to be routinely used as a sensitive probe of structure and conformation in the large number of heme and many other metalloproteins. Article in Journal/Newspaper Sperm whale University of St Andrews: Research Portal The Journal of Physical Chemistry Letters 7 8 1411 1415 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
description |
This work demonstrates the feasibility of making sensitive nanometer distancemeasurements between Fe(III) heme centers and nitroxide spin labels in proteins using the double electron-electron resonance (DEER) pulsed EPR technique at 94 GHz. Techniques to measure accurately long distances in many classes of heme proteins using DEER are currently strongly limited by sensitivity. In this paper we demonstrate sensitivity gains of more than 30times compared with previous lower frequency (X-band) DEER measurements on both human neuroglobin and sperm whale myoglobin. This is achieved by: taking advantage of recent instrumental advances; employing wideband excitation techniques based on composite pulses and exploiting more favorable relaxation properties of low-spin Fe(III) in high magnetic fields. This gain in sensitivity potentially allows the DEER technique to be routinely used as a sensitive probe of structure and conformation in the large number of heme and many other metalloproteins. |
format |
Article in Journal/Newspaper |
author |
Motion, Claire Louise Lovett, Janet Eleanor Bell, Stacey Cassidy, Scott Lindsay Cruickshank, Paul Alexander Sawchuk Bolton, David Robert Hunter, Robert Iain El Mkami, Hassane Van Doorslaer, Sabine Smith, Graham Murray |
spellingShingle |
Motion, Claire Louise Lovett, Janet Eleanor Bell, Stacey Cassidy, Scott Lindsay Cruickshank, Paul Alexander Sawchuk Bolton, David Robert Hunter, Robert Iain El Mkami, Hassane Van Doorslaer, Sabine Smith, Graham Murray DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR |
author_facet |
Motion, Claire Louise Lovett, Janet Eleanor Bell, Stacey Cassidy, Scott Lindsay Cruickshank, Paul Alexander Sawchuk Bolton, David Robert Hunter, Robert Iain El Mkami, Hassane Van Doorslaer, Sabine Smith, Graham Murray |
author_sort |
Motion, Claire Louise |
title |
DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR |
title_short |
DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR |
title_full |
DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR |
title_fullStr |
DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR |
title_full_unstemmed |
DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR |
title_sort |
deer sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field epr |
publishDate |
2016 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/deer-sensitivity-between-iron-centers-and-nitroxides-in-hemecontaining-proteins-improves-dramatically-using-broadband-highfield-epr(9edcefd2-4287-4822-a219-d87594cc1154).html https://doi.org/10.1021/acs.jpclett.6b00456 https://research-repository.st-andrews.ac.uk/bitstream/10023/8779/1/Motion_2016_DEERsensitivity_JPCL_CC.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Motion , C L , Lovett , J E , Bell , S , Cassidy , S L , Cruickshank , P A S , Bolton , D R , Hunter , R I , El Mkami , H , Van Doorslaer , S & Smith , G M 2016 , ' DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR ' , Journal of Physical Chemistry Letters , vol. 7 , no. 8 , pp. 1411-1415 . https://doi.org/10.1021/acs.jpclett.6b00456 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/deer-sensitivity-between-iron-centers-and-nitroxides-in-hemecontaining-proteins-improves-dramatically-using-broadband-highfield-epr(9edcefd2-4287-4822-a219-d87594cc1154).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1021/acs.jpclett.6b00456 |
container_title |
The Journal of Physical Chemistry Letters |
container_volume |
7 |
container_issue |
8 |
container_start_page |
1411 |
op_container_end_page |
1415 |
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1802650388222967808 |