Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology
Leptin is a multifunctional hormone, produced predominantly in adipocytes. It regulates energy balance through its impact on appetite and fat metabolism, and its concentration indicates the size of body fat reserves. Leptin also plays a vital role in stretch-induced surfactant production during alve...
Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/molecular-cloning-and-expression-of-leptin-in-gray-and-harbor-seal-blubber-bone-marrow-and-lung-and-its-potential-role-in-marine-mammal-respiratory-physiology(8778ebb6-0e6f-425d-9f80-f74af5d2c032).html https://doi.org/10.1152/ajpregu.00203.2004 http://www.scopus.com/inward/record.url?scp=23044483225&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/8778ebb6-0e6f-425d-9f80-f74af5d2c032 2024-06-23T07:53:30+00:00 Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology Hammond, J A Bennett, K A Walton, M J Hall, A J 2005-08 https://research-portal.st-andrews.ac.uk/en/researchoutput/molecular-cloning-and-expression-of-leptin-in-gray-and-harbor-seal-blubber-bone-marrow-and-lung-and-its-potential-role-in-marine-mammal-respiratory-physiology(8778ebb6-0e6f-425d-9f80-f74af5d2c032).html https://doi.org/10.1152/ajpregu.00203.2004 http://www.scopus.com/inward/record.url?scp=23044483225&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/molecular-cloning-and-expression-of-leptin-in-gray-and-harbor-seal-blubber-bone-marrow-and-lung-and-its-potential-role-in-marine-mammal-respiratory-physiology(8778ebb6-0e6f-425d-9f80-f74af5d2c032).html info:eu-repo/semantics/restrictedAccess Hammond , J A , Bennett , K A , Walton , M J & Hall , A J 2005 , ' Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 289 , no. 2 , pp. R545-R553 . https://doi.org/10.1152/ajpregu.00203.2004 surfactant production fat metabolism parathyroid hormone-related protein leptin antibody HORMONE-RELATED PROTEIN PLASMA LEPTIN NEUROPEPTIDE-Y SURFACTANT GENE ADIPOCYTES MATURATION ADIPOSITY STRETCH TISSUE article 2005 ftunstandrewcris https://doi.org/10.1152/ajpregu.00203.2004 2024-06-13T00:25:25Z Leptin is a multifunctional hormone, produced predominantly in adipocytes. It regulates energy balance through its impact on appetite and fat metabolism, and its concentration indicates the size of body fat reserves. Leptin also plays a vital role in stretch-induced surfactant production during alveolar development in the fetus. The structure, expression pattern, and role of leptin have not previously been explored in marine mammals. Phocid seals undergo cyclical changes in body composition as a result of prolonged fasting and intensive foraging bouts and experience rapid, dramatic, and repeated changes in lung volume during diving. Here, we report the tissue-specific expression pattern of leptin in these animals. This is the first demonstration of leptin expression in the lung tissue of a mature mammal, in addition to its expression in the blubber and bone marrow, in common with other animals. We propose a role for leptin in seal pulmonary surfactant production, in addition to its likely role in long-term energy balance. We identify substitutions in the phocine leptin sequence in regions normally highly conserved between widely distinct vertebrate groups, and, using a purified seal leptin antiserum, we confirm the presence of the leptin protein in gray seal lung and serum fractions. Finally, we report the substantial inadequacies of using heterologous antibodies to measure leptin in unextracted gray seal serum. Article in Journal/Newspaper harbor seal University of St Andrews: Research Portal American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 289 2 R545 R553 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
surfactant production fat metabolism parathyroid hormone-related protein leptin antibody HORMONE-RELATED PROTEIN PLASMA LEPTIN NEUROPEPTIDE-Y SURFACTANT GENE ADIPOCYTES MATURATION ADIPOSITY STRETCH TISSUE |
spellingShingle |
surfactant production fat metabolism parathyroid hormone-related protein leptin antibody HORMONE-RELATED PROTEIN PLASMA LEPTIN NEUROPEPTIDE-Y SURFACTANT GENE ADIPOCYTES MATURATION ADIPOSITY STRETCH TISSUE Hammond, J A Bennett, K A Walton, M J Hall, A J Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
topic_facet |
surfactant production fat metabolism parathyroid hormone-related protein leptin antibody HORMONE-RELATED PROTEIN PLASMA LEPTIN NEUROPEPTIDE-Y SURFACTANT GENE ADIPOCYTES MATURATION ADIPOSITY STRETCH TISSUE |
description |
Leptin is a multifunctional hormone, produced predominantly in adipocytes. It regulates energy balance through its impact on appetite and fat metabolism, and its concentration indicates the size of body fat reserves. Leptin also plays a vital role in stretch-induced surfactant production during alveolar development in the fetus. The structure, expression pattern, and role of leptin have not previously been explored in marine mammals. Phocid seals undergo cyclical changes in body composition as a result of prolonged fasting and intensive foraging bouts and experience rapid, dramatic, and repeated changes in lung volume during diving. Here, we report the tissue-specific expression pattern of leptin in these animals. This is the first demonstration of leptin expression in the lung tissue of a mature mammal, in addition to its expression in the blubber and bone marrow, in common with other animals. We propose a role for leptin in seal pulmonary surfactant production, in addition to its likely role in long-term energy balance. We identify substitutions in the phocine leptin sequence in regions normally highly conserved between widely distinct vertebrate groups, and, using a purified seal leptin antiserum, we confirm the presence of the leptin protein in gray seal lung and serum fractions. Finally, we report the substantial inadequacies of using heterologous antibodies to measure leptin in unextracted gray seal serum. |
format |
Article in Journal/Newspaper |
author |
Hammond, J A Bennett, K A Walton, M J Hall, A J |
author_facet |
Hammond, J A Bennett, K A Walton, M J Hall, A J |
author_sort |
Hammond, J A |
title |
Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
title_short |
Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
title_full |
Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
title_fullStr |
Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
title_full_unstemmed |
Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
title_sort |
molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology |
publishDate |
2005 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/molecular-cloning-and-expression-of-leptin-in-gray-and-harbor-seal-blubber-bone-marrow-and-lung-and-its-potential-role-in-marine-mammal-respiratory-physiology(8778ebb6-0e6f-425d-9f80-f74af5d2c032).html https://doi.org/10.1152/ajpregu.00203.2004 http://www.scopus.com/inward/record.url?scp=23044483225&partnerID=8YFLogxK |
genre |
harbor seal |
genre_facet |
harbor seal |
op_source |
Hammond , J A , Bennett , K A , Walton , M J & Hall , A J 2005 , ' Molecular cloning and expression of leptin in gray and harbor seal blubber, bone marrow, and lung and its potential role in marine mammal respiratory physiology ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 289 , no. 2 , pp. R545-R553 . https://doi.org/10.1152/ajpregu.00203.2004 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/molecular-cloning-and-expression-of-leptin-in-gray-and-harbor-seal-blubber-bone-marrow-and-lung-and-its-potential-role-in-marine-mammal-respiratory-physiology(8778ebb6-0e6f-425d-9f80-f74af5d2c032).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1152/ajpregu.00203.2004 |
container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
container_volume |
289 |
container_issue |
2 |
container_start_page |
R545 |
op_container_end_page |
R553 |
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1802645192644231168 |