The role of myostain and the calcineurin-signalling pathway in regulating muscle mass in response to exercise training in the rainbow trout Oncorhynchus mykiss Walbaum

• Published in: Journal of Experimental Biology
• Main Authors: Martin, C I, Johnston, Ian Alistair
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2005
• Subjects: rainbow trout; Oncorhynchus mykiss; exercise; muscle; hypertrophy; calcineurin; NFAT2; myostatin; HUMAN SKELETAL-MUSCLE; TRANSCRIPTION FACTORS; ATLANTIC SALMON; HEALTHY-MEN; BROOK TROUT; EXPRESSION; GROWTH; PROTEINS; GENE; Atlantic salmon
• Online Access: https://research-portal.st-andrews.ac.uk/en/researchoutput/the-role-of-myostain-and-the-calcineurinsignalling-pathway-in-regulating-muscle-mass-in-response-to-exercise-training-in-the-rainbow-trout-oncorhynchus-mykiss-walbaum(868215c6-4cd1-437e-9b89-bfb05b4d55b2).html; https://doi.org/10.1242/jeb.01605; http://www.scopus.com/inward/record.url?scp=20744444140&partnerID=8YFLogxK

Rainbow trout Oncorhynchus mykiss Walbaum were exercised at 0.8 and 1.6 body lengths s(-1) for 18 h a day over a 30 day period. Exercise resulted in a 24-30% increase in the average cross-sectional area of fast muscle fibres relative to tank-rested controls. The concentrations of growth factors and transcription factors hypothesised to play a role in regulating exercise-induced muscle fibre hypertrophy were measured. Exercise training resulted in a minor increase in calcineurin localisation in the nucleus. However, nuclear factor of T-cells 2 (NFAT2) nuclear localisation did not follow a pattern that was consistent with NFAT2-mediated transcriptional activity and changes in calcineurin signaling. The active peptide of myostatin, a negative regulator of muscle growth in mammals, was downregulated in exercise groups relative to tank-rested controls, but only by 6-7%. It was concluded that myostatin and calcineurin signaling do not play a major role in regulating exercise-induced muscle hypertrophy in trout.